|Validated Species:||Bovine, Human, Mouse|
Data & Images
|Product Name||Anti-Cyclin A Antibody (Monoclonal, CY-A1)|
|Description||Mouse IgG monoclonal antibody for Cyclin A, cyclin A2 (CCNA2) detection. Tested with WB, ICC in Human;mouse;bovine. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cyclin A Antibody (Monoclonal, CY-A1) CY-A1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1032)|
|Replacement Item||This antibody may replace the following items: sc-136253|sc-239|sc-239-X|sc-271645|sc-271645-X|sc-271682|sc-271682-X|sc-31084|sc-31085|sc-31086|sc-53227|sc-53228|sc-53229|sc-53230|sc-53231|sc-53232|sc-596|sc-596-G|sc-751|sc-751-X from Santa Cruz Biotechnology.|
|Validated Species||Bovine, Human, Mouse|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant bovine cyclin A.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||47269 MW|
|Protein Function||Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. .|
|Tissue Specificity||Ubiquitous. In the testis, expressed in germ cells and in the ovary, in both germline and somatic cells.|
|Sequence Similarities||Belongs to the cyclin family. Cyclin AB subfamily.|
|Subcellular Localization||Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER. .|
|Alternative Names||Cyclin-A2;Cyclin-A;Ccna2;Ccna, Cyca;|
|Research Areas|||cell biology|cell cycle|cyclins| cell biology|cyclin a family| epigenetics and nuclear signaling| cancer| cardiovascular|lipids / lipoproteins|lipoproteins/apolipoproteins||
Background for Cyclin-A2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Cyclin A Antibody (Monoclonal, CY-A1) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 1μg/ml, Human, bovine , mouse, -|
Western blot, 0.5-1μg/ml, Human, bovine , mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Cyclin A Antibody (Monoclonal, CY-A1) Images
Click the images to enlarge.
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
ICC: A549 Cell
ICC: SMMC-7721 Cell