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Overview
| Product Name | Anti-Cyclin A Antibody (Monoclonal, CY-A1) |
|---|---|
| SKU/Catalog Number | MA1032 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for Cyclin A, cyclin A2 (CCNA2) detection. Tested with WB, ICC in Human;mouse;bovine. No cross reactivity with other proteins. |
| Cite This Product | Anti-Cyclin A Antibody (Monoclonal, CY-A1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1032) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Immunogen | Recombinant bovine cyclin A. |
| Reactivity | Bovine, Human, Mouse |
Assay Details
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunocytochemistry , 1μg/ml, Human, bovine , mouse, -
Western blot, 0.5-1μg/ml, Human, bovine , mouse
Immunocytochemistry , 1μg/ml, Human, bovine , mouse, -
Western blot, 0.5-1μg/ml, Human, bovine , mouse
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.Images And Assay Conditions
Anti-Cyclin A antibody (monoclonal), MA1032, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Anti-Cyclin A antibody (monoclonal), MA1032, ICC
ICC: A549 Cell
Anti-Cyclin A antibody (monoclonal), MA1032, ICC
ICC: SMMC-7721 Cell
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P51943 |
|---|---|
| Gene Name | CCNA2 |
| Protein Name | Cyclin-A2 |
| Tissue Specificity | Ubiquitous. In the testis, expressed in germ cells and in the ovary, in both germline and somatic cells. |
| Alternative Names | Cyclin-A2;Cyclin-A;Ccna2;Ccna, Cyca; |
| Subcellular Localization | Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER. . |
| Molecular Weight | 47269 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. . |
|---|---|
| Research Areas | Cancer, Cardiovascular, Cell Biology, Cell Cycle, Epigenetics And Nuclear Signaling, Lipids / Lipoproteins *You can search these to find other products in these research areas. |
| Background | The mammalian A-type cyclin family consists of 2 members, cyclin A1(CCNA1) and cyclin A2(CCNA2). Human cyclin A gene is assigned to 4q26-q27. Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts. |
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (MA1032)
Promotion:
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
Publications
A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1/CIP1 Expression
Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells
Mao F, Wang B, Xiao Q, Xi G, Sun W, Zhang H, Ye F, Wan F, Guo D, Lei T, Chen X. Int J Oncol. 2013 Mar;42(3):1081-7. Doi: 10.3892/Ijo.2013.1776. Epub 2013 Jan 16. A Role For Lrig1 In The Regulation Of Malignant Glioma Aggressiveness.
Lin P, Fu J, Zhao B, Lin F, Zou H, Liu L, Zhu C, Wang H, Yu X. Mol Biol Rep. 2011 Mar;38(3):1741-7. Doi: 10.1007/S11033-010-0288-7. Epub 2010 Sep 29. Fbxw8 Is Involved In The Proliferation Of Human Choriocarcinoma Jeg-3 Cells.
Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Guo X. Biochem Biophys Res Commun. 2013 Oct 18;440(2):322-9. Doi: 10.1016/J.Bbrc.2013.09.079. Epub 2013 Sep 25. Ran Gtpase Protein Promotes Human Pancreatic Cancer Proli...
Zhao P, Li Y, Gao G, Wang S, Yan Y, Zhan X, Liu Z, Mao Z, Chen S, Wang L. Eur J Med Chem. 2014 Oct 30;86:165-74. Doi: 10.1016/J.Ejmech.2014.08.049. Epub 2014 Aug 15. Design, Synthesis And Biological Evaluation Of N-Alkyl Or Aryl Substituted Isoind...
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cyclin-a antibody, ccn1 antibody, cyclin a2 antibody, cyclin-a2 antibody