Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)

Western blot analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclin A antigen affinity purified monoclonal antibody (Catalog # MA1032) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cyclin A at approximately 55 kDa. The expected band size for Cyclin A is at 49 kDa.

AIF blocks hypoxia-induced progression of PH in vitro and in vivo. A Hypoxia increased the viability of PASMCs after growth arrest for 24 h, and this effect was decreased by AIF (n = 4). B Pretreatment with an AIF overexpression plasmid blocked the effects of hypoxia on EdU incorporation in cells (n = 6). Scale bars: 50 μm. C Cell cycle analysis by flow cytometry indicated that hypoxia stimulated cell progression into G 2 /M + S phase, and this effect was inhibited by AIF overexpression (n = 3). D Effects of AIF on the expression of PCNA, Cyclin A and Cyclin D under hypoxia (n = 4–5). E Represents weight ratio of the right ventricular (RV)/left ventricular (LV) + Septum (n = 6); F Represents the right ventricular systolic pressure (RVSP) from mice (n = 5); G pulmonary artery velocity time integral (PAVTI), pulmonary artery acceleration time (PAAT) and left ventricular ejection fraction (LVEF) of the hypoxic mouse model infected with AAV5-NC and AAV5-AIF (n = 6). All data are presented as the means ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001; Nor normoxia, Hyp hypoxia, NC negative control
Index in PubMed under a CC BY license. PMID: 35090552

ICC analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Cyclin A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

ICC analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Cyclin A was detected in an immunocytochemical section of SMMC-7721 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.