Anti-Cyclin A Antibody (Monoclonal, CY-A1)

Mouse IgG monoclonal antibody for Cyclin A, cyclin A2 (CCNA2) detection. Tested with WB, ICC in Human;mouse;bovine. No cross reactivity with other proteins.

SKU MA1032
Size 100μg/vial
Reactivity Bovine, Human, Mouse
Clonality Monoclonal
Clone Number CY-A1
Host Mouse
Ig Isotype Mouse IgG2a
Applications ICC, WB

Overview

Product Name Anti-Cyclin A Antibody (Monoclonal, CY-A1)
See all Ccna2 primary antibodies, ELISA kits and proteins
SKU/Catalog Number MA1032
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Monoclonal clone# G2 antibody for CYCLIN A/CCNA2 detection. Host: Mouse.Size: 100μg/vial. Tested applications: ICC. Reactive species: Bovine. CYCLIN A/CCNA2 information: Molecular Weight: 47269 MW; Subcellular Localization: Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER; Tissue Specificity: Ubiquitous. In the testis, expressed in germ cells and in the ovary, in both germline and somatic cells.
Cite This Product Anti-Cyclin A Antibody (Monoclonal, CY-A1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1032)
Specificity Anti-Cyclin A Antibody (Monoclonal, CY-A1) (MA1032) reacts with Bovine, Human, Mouse Ccna2, in native form and recombinant. Superfamily members of Ccna2 are not reactive to MA1032.
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunogen Recombinant bovine cyclin A.
Reactivity Bovine, Human, Mouse

Assay Details

Our Boster Quality Guarantee for Anti-Cyclin A Antibody (Monoclonal, CY-A1) covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunocytochemistry , 1μg/ml, Human, bovine , mouse, -
Western blot, 0.5-1μg/ml, Human, bovine , mouse

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-Cyclin A Antibody (Monoclonal, CY-A1) (MA1032).

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Images And Assay Conditions

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Anti-Cyclin A antibody (monoclonal), MA1032, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate

/antibody/ma1032-3-IF-anti-cyclin-a-antibody-monoclonal-cy-a1.jpg

Anti-Cyclin A antibody (monoclonal), MA1032, ICC
ICC: SMMC-7721 Cell

/antibody/ma1032-2-IF-anti-cyclin-a-antibody-monoclonal-cy-a1.jpg

Anti-Cyclin A antibody (monoclonal), MA1032, ICC
ICC: A549 Cell

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P51943
Gene Name Ccna2
Protein Name Cyclin-A2
Tissue Specificity Ubiquitous. In the testis, expressed in germ cells and in the ovary, in both germline and somatic cells.
Alternative Names Cyclin-A2;Cyclin-A;Ccna2;Ccna, Cyca;
Subcellular Localization Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER. .
Molecular Weight 47269 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. .
Research Areas Bovine, Human, Mouse

*You can search these to find other products in these research areas.
Background The mammalian A-type cyclin family consists of 2 members, cyclin A1(CCNA1) and cyclin A2(CCNA2). Human cyclin A gene is assigned to 4q26-q27. Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts.

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Publishing with Anti-Cyclin A Antibody (Monoclonal, CY-A1) (MA1032)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]

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In stock
Order Product
MA1032
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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We promise all of our products perform as described in datasheets.

Publications

Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells
Wang C, Ge Q, Chen Z, Hu J, Li F, Song X, Xu H, Ye Z. Biomed Res Int. 2015;2015:304753. Doi: 10.1155/2015/304753. Epub 2015 Mar 30. A New Double Stranded Rna Suppresses Bladder Cancer Development By Upregulating P21 (Waf1/Cip1) Expression.
Mao F, Wang B, Xiao Q, Xi G, Sun W, Zhang H, Ye F, Wan F, Guo D, Lei T, Chen X. Int J Oncol. 2013 Mar;42(3):1081-7. Doi: 10.3892/Ijo.2013.1776. Epub 2013 Jan 16. A Role For Lrig1 In The Regulation Of Malignant Glioma Aggressiveness.
Lin P, Fu J, Zhao B, Lin F, Zou H, Liu L, Zhu C, Wang H, Yu X. Mol Biol Rep. 2011 Mar;38(3):1741-7. Doi: 10.1007/S11033-010-0288-7. Epub 2010 Sep 29. Fbxw8 Is Involved In The Proliferation Of Human Choriocarcinoma Jeg-3 Cells.
Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Guo X. Biochem Biophys Res Commun. 2013 Oct 18;440(2):322-9. Doi: 10.1016/J.Bbrc.2013.09.079. Epub 2013 Sep 25. Ran Gtpase Protein Promotes Human Pancreatic Cancer Proli...
Zhao P, Li Y, Gao G, Wang S, Yan Y, Zhan X, Liu Z, Mao Z, Chen S, Wang L. Eur J Med Chem. 2014 Oct 30;86:165-74. Doi: 10.1016/J.Ejmech.2014.08.049. Epub 2014 Aug 15. Design, Synthesis And Biological Evaluation Of N-Alkyl Or Aryl Substituted Isoind...
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to cyclin-a antibody, ccn1 antibody, cyclin a2 antibody, cyclin-a2 antibody
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