Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)

Product Name

Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)

View all Cyclin A2 Antibodies

SKU/Catalog Number

MA1032

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) catalog # MA1032. Tested in ICC, WB applications. This antibody reacts with Bovine, Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1032)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

CY-A1

Isotype

Mouse IgG2a

Immunogen

Recombinant bovine cyclin A.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1032 is reactive to Ccna2 in Bovine, Human, Mouse

Applications

MA1032 is guaranteed for ICC, WB Boster Guarantee

Background of Cyclin A2

The mammalian A-type cyclin family consists of 2 members, cyclin A1 (CCNA1) and cyclin A2 (CCNA2). Human cyclin A gene is assigned to 4q26-q27. Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunocytochemistry , 1μg/ml, Human, bovine , mouse, -
Western blot, 0.5-1μg/ml, Human, bovine , mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclin A antigen affinity purified monoclonal antibody (Catalog # MA1032) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cyclin A at approximately 55 kDa. The expected band size for Cyclin A is at 49 kDa.

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Figure 2. ICC analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Cyclin A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

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Figure 3. ICC analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Cyclin A was detected in an immunocytochemical section of SMMC-7721 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Gene/Protein Information For Ccna2 (Source: Uniprot.org, NCBI)

Alternative Names

CCN1CCNAcyclin-A; CCNA; CCNA2; Cyclin A2; Cyclin-A; cyclin-A2 Ccna2|AA408589, Cc, Ccn, Ccn-, Ccn-1, Ccn1, Ccna, Cyc, CycA2, Cyca|cyclin A2|cyclin-A2|Cyclin A2 (Cyclin A)|cyclin-A

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In this infographic, you will see the following information for Ccna2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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