Anti-Cyclin A Antibody (Monoclonal, CY-A1)

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Datasheet

Protocols

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SKU MA1032
Size 100μg/vial
Reactivity Bovine, Human, Mouse
Clonality Monoclonal
Clone Number CY-A1
Host Mouse
Ig Isotype Mouse IgG2a
Applications ICC, WB

Overview

Product Name Anti-Cyclin A Antibody (Monoclonal, CY-A1)
SKU/Catalog Number MA1032
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Cyclin A, cyclin A2 (CCNA2) detection. Tested with WB, ICC in Human;mouse;bovine. No cross reactivity with other proteins.
Cite This Product Anti-Cyclin A Antibody (Monoclonal, CY-A1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1032)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Recombinant bovine cyclin A.
Reactivity Bovine, Human, Mouse

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunocytochemistry , 1μg/ml, Human, bovine , mouse, -
Western blot, 0.5-1μg/ml, Human, bovine , mouse

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Images And Assay Conditions

/antibody/ma1032 1 WB anti cyclin a antibody monoclonal cy a1.jpg

Anti-Cyclin A antibody (monoclonal), MA1032, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate

/antibody/ma1032 2 IF anti cyclin a antibody monoclonal cy a1.jpg

Anti-Cyclin A antibody (monoclonal), MA1032, ICC
ICC: A549 Cell

/antibody/ma1032 3 IF anti cyclin a antibody monoclonal cy a1.jpg

Anti-Cyclin A antibody (monoclonal), MA1032, ICC
ICC: SMMC-7721 Cell

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P51943
Gene Name CCNA2
Protein Name Cyclin-A2
Tissue Specificity Ubiquitous. In the testis, expressed in germ cells and in the ovary, in both germline and somatic cells.
Alternative Names Cyclin-A2;Cyclin-A;Ccna2;Ccna, Cyca;
Subcellular Localization Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER. .
Molecular Weight 47269 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. .
Research Areas Cancer, Cardiovascular, Cell Biology, Cell Cycle, Epigenetics And Nuclear Signaling, Lipids / Lipoproteins

*You can search these to find other products in these research areas.
Background The mammalian A-type cyclin family consists of 2 members, cyclin A1(CCNA1) and cyclin A2(CCNA2). Human cyclin A gene is assigned to 4q26-q27. Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts.

Other Recommended Resources

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Order Product (MA1032)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240

USD $240

Ships in 7-10 business days.

Troubleshooting

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Publications

A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1/CIP1 Expression
PubMed ID25918708
Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells
PubMed ID24628936
Mao F, Wang B, Xiao Q, Xi G, Sun W, Zhang H, Ye F, Wan F, Guo D, Lei T, Chen X. Int J Oncol. 2013 Mar;42(3):1081-7. Doi: 10.3892/Ijo.2013.1776. Epub 2013 Jan 16. A Role For Lrig1 In The Regulation Of Malignant Glioma Aggressiveness.
PubMed ID23337938
Lin P, Fu J, Zhao B, Lin F, Zou H, Liu L, Zhu C, Wang H, Yu X. Mol Biol Rep. 2011 Mar;38(3):1741-7. Doi: 10.1007/S11033-010-0288-7. Epub 2010 Sep 29. Fbxw8 Is Involved In The Proliferation Of Human Choriocarcinoma Jeg-3 Cells.
PubMed ID20878477
Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Guo X. Biochem Biophys Res Commun. 2013 Oct 18;440(2):322-9. Doi: 10.1016/J.Bbrc.2013.09.079. Epub 2013 Sep 25. Ran Gtpase Protein Promotes Human Pancreatic Cancer Proli...
PubMed ID24076388
Zhao P, Li Y, Gao G, Wang S, Yan Y, Zhan X, Liu Z, Mao Z, Chen S, Wang L. Eur J Med Chem. 2014 Oct 30;86:165-74. Doi: 10.1016/J.Ejmech.2014.08.049. Epub 2014 Aug 15. Design, Synthesis And Biological Evaluation Of N-Alkyl Or Aryl Substituted Isoind...
PubMed ID25151579
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...
PubMed ID24194897

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cyclin-a antibody, ccn1 antibody, cyclin a2 antibody, cyclin-a2 antibody