Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)

Boster Bio Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) catalog # MA1032. Tested in ICC, WB applications. This antibody reacts with Bovine, Human, Mouse. Cited in 8 publication(s).

Product Info Summary

SKU: MA1032
Size: 100μg/vial
Reactive Species: Bovine, Human, Mouse
Host: Mouse
Application: ICC, WB

Product Name

Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)

See all Cyclin A2 products

SKU/Catalog Number

MA1032

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) catalog # MA1032. Tested in ICC, WB applications. This antibody reacts with Bovine, Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1032)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

CY-A1

Isotype

Mouse IgG2a

Immunogen

Recombinant bovine cyclin A.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1032 is reactive to Ccna2 in Bovine, Human, Mouse

Applications

MA1032 is guaranteed for ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunocytochemistry , 1μg/ml, Human, bovine , mouse, -
Western blot, 0.5-1μg/ml, Human, bovine , mouse

Validation Images & Assay Conditions

Gene/Protein Information For Ccna2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P51943

Gene ID

12428

Gene Name

Ccna2

Full Name

Cyclin-A2

Weight

47.269kDa

Superfamily

cyclin family

Alternative Names

CCN1CCNAcyclin-A; CCNA; CCNA2; Cyclin A2; Cyclin-A; cyclin-A2 Ccna2|AA408589, Cc, Ccn, Ccn-, Ccn-1, Ccn1, Ccna, Cyc, CycA2, Cyca|cyclin A2|cyclin-A2|Cyclin A2 (Cyclin A)|cyclin-A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccna2, check out the Ccna2 Infographic

Ccna2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccna2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1032 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang S,Zhang C,Zhang X.Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells.Mol Med Rep.2020 Feb;21(2):589-596.doi:10.3892/mmr.2019.10887.Epub 2019 Dec 17.PMID:31974617; PMC
Species: Human
MA1032 usage in article: APP:WB, SAMPLE:HPASMCS, DILUTION:1:200

Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells

Wang C, Ge Q, Chen Z, Hu J, Li F, Song X, Xu H, Ye Z. Biomed Res Int. 2015;2015:304753. Doi: 10.1155/2015/304753. Epub 2015 Mar 30. A New Double Stranded Rna Suppresses Bladder Cancer Development By Upregulating P21 (Waf1/Cip1) Expression.

Mao F, Wang B, Xiao Q, Xi G, Sun W, Zhang H, Ye F, Wan F, Guo D, Lei T, Chen X. Int J Oncol. 2013 Mar;42(3):1081-7. Doi: 10.3892/Ijo.2013.1776. Epub 2013 Jan 16. A Role For Lrig1 In The Regulation Of Malignant Glioma Aggressiveness.

Lin P, Fu J, Zhao B, Lin F, Zou H, Liu L, Zhu C, Wang H, Yu X. Mol Biol Rep. 2011 Mar;38(3):1741-7. Doi: 10.1007/S11033-010-0288-7. Epub 2010 Sep 29. Fbxw8 Is Involved In The Proliferation Of Human Choriocarcinoma Jeg-3 Cells.

Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Guo X. Biochem Biophys Res Commun. 2013 Oct 18;440(2):322-9. Doi: 10.1016/J.Bbrc.2013.09.079. Epub 2013 Sep 25. Ran Gtpase Protein Promotes Human Pancreatic Cancer Proli...

Zhao P, Li Y, Gao G, Wang S, Yan Y, Zhan X, Liu Z, Mao Z, Chen S, Wang L. Eur J Med Chem. 2014 Oct 30;86:165-74. Doi: 10.1016/J.Ejmech.2014.08.049. Epub 2014 Aug 15. Design, Synthesis And Biological Evaluation Of N-Alkyl Or Aryl Substituted Isoind...

Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...

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6 Customer Q&As for Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)

Question

Would MA1032 anti-Cyclin A antibody (Monoclonal, CY-A1) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

It shows on the product datasheet, MA1032 anti-Cyclin A antibody (Monoclonal, CY-A1) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-06

Question

Do you have a BSA free version of anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 available?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-22

Question

Here is the WB image, lot number and protocol we used for erythroleukemia using anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-11

Question

Will anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 work on canine ICC with brain?

Verified Customer

Verified customer

Asked: 2019-03-07

Answer

Our lab technicians have not validated anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in ICC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-07

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032. Let me know if you need anything else.

T. Li

Verified customer

Asked: 2017-07-28

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-28

Question

I see that the anti-Cyclin A antibody (Monoclonal, CY-A1) MA1032 works with WB, what is the protocol used to produce the result images on the product page?

C. Mitchell

Verified customer

Asked: 2016-07-14

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-07-14

Size

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MA1032
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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