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- Table of Contents
Ccna2 antibody (MA1032) performs well in ICC, WB and shows reactivity with Bovine, Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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ICC analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Cyclin A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Cyclin A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)
Ccna2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Bovine, Human, Mouse | Mouse | ICC, WB |
Tested Applications
Ccna2 antibody was positively detected by WB in:
human Hela whole cell, human Jurkat whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Ccna2 antibody was positively detected by ICC in:
A549 cell, SMMC-7721 cell
Recommend Dilution
Application
Dilution
Species
Immunocytochemistry
1μg/ml
Human, bovine, mouse, -
Western blot
0.5-1μg/ml
Human, bovine, mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)
View all CCNA2 Antibodies
View all CCNA2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
CY-A1
Isotype
Mouse IgG2a
Immunogen
Recombinant bovine cyclin A.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
47.3 kDa
Background of Ccna2
The mammalian A-type cyclin family consists of 2 members, cyclin A1 (CCNA1) and cyclin A2 (CCNA2). Human cyclin A gene is assigned to 4q26-q27. Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts.
Cite This Product
Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1032)
Protocols
Western Blot protocol for Ccna2 antibody
Immunocytochemistry (ICC) protocol for Ccna2 antibody
Publications
Application:
Species:
Super-Enhancer Target Gene CBP/p300-Interacting Transactivator With Glu/Asp-Rich C-Terminal Domain, 2 Cooperates With Transcription Factor Forkhead Box J3 to Inhibit Pulmonary Vascular Remodeling
Published: 2025-02-05
Journal: CELL PROLIFERATION
Application: WB
Impact Factor: 5.60
Species: Mouse,Human
PMID: 39907030
Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly
Published: 2022-02-11
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Application: IF
Impact Factor: 6.51
Species: Human
PMID: 35147977
Ubiquitinated AIF is a major mediator of hypoxia-induced mitochondrial dysfunction and pulmonary artery smooth muscle cell proliferation
Published: 2022-01-28
Journal: Cell and Bioscience
Application: WB
Impact Factor: 9.58
Species: Rat
PMID: 35090552
Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells
Published: 2021-01-01
Journal: ZOOLOGICAL RESEARCH
Application: WB
Impact Factor: 4.56
Species: Mouse
PMID: 33420764
piRNA-63076 contributes to pulmonary arterial smooth muscle cell proliferation through acyl-CoA dehydrogenase
Published: 2020-03-30
Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Application: WB
Impact Factor: 4.49
Species: Rat
PMID: 32227582
Haspin inhibition delays cell cycle progression through interphase in cancer cells
Published: 2019-10-17
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Application: WB
Impact Factor: 4.52
Species: Human
PMID: 31625162
MiR-449a-5p mediates mitochondrial dysfunction and phenotypic transition by targeting Myc in pulmonary arterial smooth muscle cells
Published: 2019-02-04
Journal: JOURNAL OF MOLECULAR MEDICINE-JMM
Application: WB
Impact Factor: 4.75
Species: Rat
PMID: 30715622
Long noncoding RNA Hoxaas3 contributes to hypoxia-induced pulmonary artery smooth muscle cell proliferation
Published: 2018-10-09
Journal: CARDIOVASCULAR RESEARCH
Application: WB
Impact Factor: 6.29
Species: Mouse
PMID: 30304383
MiR-125a regulates mitochondrial homeostasis through targeting mitofusin 1 to control hypoxic pulmonary vascular remodeling
Published: 2017-06-07
Journal: JOURNAL OF MOLECULAR MEDICINE-JMM
Application: WB
Impact Factor: 4.69
Species: Rat
PMID: 28593577
Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells
Published: 2014-03-17
Journal: Cell Communication and Signaling
Application: WB
Impact Factor: 4.67
Species: Rat
PMID: 24628936
The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells
Published: 2013-01-22
Journal: CANCER LETTERS
Application: WB
Impact Factor: 4.26
Species: Human
PMID: 23352642
A role for LRIG1 in the regulation of malignant glioma aggressiveness
Published: 2013-01-16
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY
Application: WB
Impact Factor: 2.66
Species: Human
PMID: 23337938
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Product Promise
We promise Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) performs excellently in relevant applications for ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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MA1032
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026