Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FGF21 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EZ0994
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all FGF-21 products

SKU/Catalog Number

EZ0994

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FGF21 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0994)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H29-S209

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0994 is reactive to FGF21 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Application Guarantee

EZ0994 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0994-CA Rabbit anti- human FGF21 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0994-DA Biotinylated goat anti- human FGF21 polyclonal antibody (Detection Antibody) 500 µl, 2.5 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0994-ST Lyophilized recombinant human FGF21 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0170.0630.1030.1950.3850.7121.4222.24

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)25664514782476491426
Standard deviation11.5221.9369.4724.4548.0389.84
CV (%)4.5%3.4%4.7%9.9%7.4%6.3%

Gene/Protein Information For FGF21 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NSA1

Gene ID

26291

Gene Name

FGF21

Full Name

Fibroblast growth factor 21

Weight

22.3kDa

Superfamily

heparin-binding growth factors family

Alternative Names

FGF21; FGF-21; fibroblast growth factor 21 FGF21 fibroblast growth factor 21 fibroblast growth factor 21

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FGF21, check out the FGF21 Infographic

FGF21 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FGF21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Question

What is the recommended sample diluent for EZ0994?

Verified customer

Asked: 2019-05-09

Answer

The recommended sample diluent of the Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0994) for serum is 1:1.

Boster Scientific Support

Answered: 2019-05-10

Question

Do you recommend to include 0.05% Tween 20 v/v in the wash buffer and do you foresee any issue?

Verified customer

Asked: 2019-05-09

Answer

For the Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0994), you can include 0.05% Tween 20 v/v in the wash buffer. It is OK to use 1x PBS with NaCl, KCl, Na2HPO4 and KH2PO4.

Boster Scientific Support

Answered: 2019-05-10

Question

Is washing recommended after coating (before blocking) for EZ0994?

Verified customer

Asked: 2019-05-09

Answer

For the Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0994), washing or not washing would not make too much difference to the assay result.

Boster Scientific Support

Answered: 2019-05-10

Question

Do you recommend washing step after sample/standard/control incubation int the assay procedure for EZ0994?

Verified customer

Asked: 2019-05-09

Answer

No, we do not recommend washing step after sample/standard/control incubation the Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0994).

Boster Scientific Support

Answered: 2019-05-10

Question

Would you recommend using PBS pH7.2 – 7.4 or Sodium Bicarb buffer pH 9.6 for EZ0994?

Verified customer

Asked: 2019-05-09

Answer

Both PBS pH7.2 – 7.4 and Sodium Bicarb buffer pH 9.6 are commonly used capture antibody diluent. Our lab hasn't validated their performance. If you prefer, please run a pilot test to validate their effect. We recommend using "Capture Antibody Diluent: 100 mL H2O, 0.5 g Na2HPO4", which is stated on our datasheet.

Boster Scientific Support

Answered: 2019-05-10

Question

Is there pH requirement for “Capture Antibody Diluent: 100 mL H2O, 0.5 g Na2HPO4”?

Verified customer

Asked: 2019-05-07

Answer

The pH value should be 8.0-9.0.

Boster Scientific Support

Answered: 2019-05-07

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