Mouse FGF21 ELISA Kit PicoKine®

FGF-21 ELISA kit for Mouse

Mouse FGF21 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fgf21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK1379
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse FGF21 ELISA Kit PicoKine®

View all FGF-21 ELISA kits

SKU/Catalog Number

EK1379

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse FGF21 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fgf21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse FGF21 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1379)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A29-S210

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1379 is reactive to Fgf21 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1379 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of FGF-21

Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

Kit Components

Catalog Number Description Quantity
EK1379-CAP Anti-Mouse Fgf21 Pre-coated 96-well strip microplate 1
EK1379-ST Mouse Fgf21 Standard 2 vials, 10 ng/tube
EK1379-DA Mouse Fgf21 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0060.0740.1340.2480.4840.8031.3551.936

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect FGF-21, Dilution ratio of 1:1, concentration in serum is 500-700pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse FGF21 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6432091865289938
Standard deviation2.812452.323.1227.1670.35
CV (%)4.4%7.5%5.7%4.8%9.4%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of FGF-21 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 164626154603.766.2%
Sample 232028531029630213.324.4%
Sample 391892896498194725.712.7%
*number of samples for each test n=16.

Gene/Protein Information For Fgf21 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9JJN1

Gene ID

56636

Gene Name

Fgf21

Full Name

Fibroblast growth factor 21

Weight

23.237kDa

Superfamily

heparin-binding growth factors family

Alternative Names

FGF21; FGF-21; fibroblast growth factor 21 Fgf21|Fgf8c|fibroblast growth factor 21|fibroblast growth factor 21|fibroblast growth factor 8c

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Fgf21, check out the Fgf21 Infographic

Fgf21 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Fgf21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1379 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

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Liu M, Pan Q, Chen Y, Yang X, Zhao B, Jia L, Zhu Y, Zhang B, Gao X, Li X, Han J, Duan Y. Sci Rep. 2015 Jun 10;5:11219. Doi: 10.1038/Srep11219. Administration Of Danhong Injection To Diabetic Db/Db Mice Inhibits The Development Of Diabetic Retinopa...

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5 Customer Q&As for Mouse FGF21 ELISA Kit PicoKine®

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Green

Verified customer

Asked: 2020-08-22

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-08-22

Question

Q: how do I analyze ELISA data? I have obtained FGF-21 level in serum.

K. Patel

Verified customer

Asked: 2019-11-03

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-11-03

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement FGF-21 using the EK1379 Mouse FGF-21 Picokine® ELISA Kit?

D. Varma

Verified customer

Asked: 2018-10-26

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-10-26

Question

We have a customer who wants to purchase your Human PROS1 PicoKine ELISA Kit EK1739-96WELLS because they are interested in the human PROS1. However, at a later time point maybe also PROS1 in mice. Do you have any data if the kit might work with mice? Normally you say if the sequence if the sequences are 90%+ identical, there is a high chance of cross-reactivity

Verified Customer

Verified customer

Asked: 2017-06-08

Answer

Our lab technicians didn't detect PROS1 in serum from healthy mice. Best Regards,

Boster Scientific Support

Answered: 2017-06-08

Question

I only have 20 µL of plasma sample. Can I dilute the sample to 100 µL for this assay?

Verified Customer

Verified customer

Asked: 2014-08-14

Answer

Yes if the sample is mouse plasma. Note that we do not recommend this dilution for human plasma because its FGF21 level is lower than that in mouse plasma.

Boster Scientific Support

Answered: 2014-08-14

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