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Product Info Summary
| SKU: | A03790 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-Smac/Diablo Antibody
SKU/Catalog Number
A03790
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Smac/Diablo Antibody (Catalog # A03790). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Smac/Diablo Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03790)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03790 is reactive to DIABLO in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
27.1 kDa
Background of DIABLO
Apoptosis is a conserved cell suicide program essential for the development and homeostasis of multi-cellular organisms. Abnormal inhibition of apoptosis is a hallmark of cancer and autoimmune diseases, whereas excessive cell death is found in neurodegenerative disorders such as Alzheimers disease. Executioners of the apoptotic program are cysteine proteases termed caspases that exist as inactive zymogens in living cells and are activated during apoptosis. Active caspases cleave key intracellular protein substrates, resulting in the characteristic morphological changes associated with apoptosis. The release of cytochrome c from the mitochondria triggers the oligomerization of Apaf-1 in an ATP/dATP-dependent manner and induces the autoactivation of caspase-9. Active caspase-9 in turn activates downstream effector caspases including caspase -3, -6 and -7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03790 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IP: 1:100
WB: 1:1,000 - 1:2,000
This antibody was tested by immunoblot and reacts with human Smac/DIABLO protein. Lysates from human HeLa and LNCaP cells are positive for Smac/DIABLO. Other animal tissues have not been tested.
Validation Images & Assay Conditions
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Western blot using anti-Smac detects a 26 kDa band when 1 µg of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 µg of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 µg each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 µM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1’
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Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1’. Other detection systems will yield similar results.
Specific Publications For Anti-Smac/Diablo Antibody (A03790)
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