Human IL-11/Interleukin-11 ELISA Kit PicoKine™

Human IL-11 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL11 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0419
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human IL-11/Interleukin-11 ELISA Kit PicoKine™

See all IL-11 products

SKU/Catalog Number

EK0419

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-11 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL11 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-11/Interleukin-11 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0419)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: P22-L199

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0419 is reactive to IL11 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0419 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0419-CAP Anti-Human IL11 Pre-coated 96-well strip microplate 1
EK0419-ST Human IL11 Standard 2 vials, 10 ng/tube
EK0419-DA Human IL11 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.1520.2310.2710.3450.5070.8121.3332.186

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-11, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-11 in samples: 12032273 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-11/Interleukin-11 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7029088267281907
Standard deviation3.2220.342.333.8123.0445.35
CV (%)4.6%7%4.8%5.7%8.2%5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-11 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 170657974725.147.1%
Sample 229028630326928712.144.2%
Sample 388276178689383057.816.9%
*number of samples for each test n=16.

Gene/Protein Information For IL11 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20809

Gene ID

3589

Gene Name

IL11

Full Name

Interleukin-11

Weight

21.429kDa

Superfamily

IL-6 superfamily

Alternative Names

Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin IL11 AGIF, IL-11 interleukin 11 interleukin-11|adipogenesis inhibitory factor|oprelvekin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL11, check out the IL11 Infographic

IL11 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0419 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Gioxari A,Amerikanou C,Papada E,Zioga E,Georgoulis AD,Bamias G,Kaliora AC.Serum Vitamins D, B9 and B12 in Greek Patients with Inflammatory Bowel Diseases. Nutrients. 2020 Dec 4;12(12):E3734.doi:10.3390 /nu12123734.PMID:33291539.
Species: Human
EK0419 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

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Have a question?

Find answers in Q&As, reviews.

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7 Customer Q&As for Human IL-11/Interleukin-11 ELISA Kit PicoKine™

Question

Q: how to proceed with the analysis of ELISA data? I have performed IL-11 level in serum.

K. Gonzalez

Verified customer

Asked: 2019-12-23

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-12-23

Question

Q: is it okay to use EDTA plasma as samples in Human IL-11 Picokine® ELISA Kit (Catalog # EK0419)?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-11 causing degradation of its protein structure. IL-11 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-11 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-07-11

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring IL-11 using the EK0419 Human IL-11 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-04-25

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2019-04-25

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of IL-11 in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-10-17

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-11 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-10-17

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

M. Kumar

Verified customer

Asked: 2018-02-14

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-02-14

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-02-11

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-02-11

Question

Q: if the enzyme conjugated IL-11 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-11 antigen?

Verified Customer

Verified customer

Asked: 2017-10-16

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-10-16

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