Anti-Cytochrome C CYCS Antibody Picoband™ (monoclonal, 15F10)

Cytochrome c antibody

Boster Bio Anti-Cytochrome C CYCS Antibody Picoband™ (monoclonal, 15F10) catalog # M03529-5. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: M03529-5
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Cytochrome C CYCS Antibody Picoband™ (monoclonal, 15F10)

View all Cytochrome c Antibodies

SKU/Catalog Number

M03529-5

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cytochrome C CYCS Antibody Picoband™ (monoclonal, 15F10) catalog # M03529-5. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytochrome C CYCS Antibody Picoband™ (monoclonal, 15F10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03529-5)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

15F10

Isotype

Mouse IgG1

Immunogen

E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M03529-5 is reactive to Cycs in Human, Mouse, Rat

Applications

M03529-5 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

14 kDa

Calculated molecular weight

11.605kDa

Background of Cytochrome c

CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For Cycs (Source: Uniprot.org, NCBI)

Gene Name

Cycs

Full Name

Cytochrome c, somatic

Weight

11.605kDa

Superfamily

cytochrome c family

Alternative Names

CYCHCS; CYCS; Cytochrome c; cytochrome c, somatic; THC4 Cycs|cytochrome c, somatic|cytochrome c, somatic

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cycs, check out the Cycs Infographic

Cycs infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cycs: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M03529-5 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang Z,Wang J,Zhu Y,Zhang H,Wang H.Astragaloside IV alleviates myocardial damage induced by type 2 diabetes via improving energy metabolism.Mol Med Rep.2019 Nov;20(5):4612-4622.doi:10.3892/mmr.2019.10716.Epub 2019 Oct 1.PMID:31702040; PMCID:PMC6797977.
Species: Human,Rat
M03529-5 usage in article: APP:WB, SAMPLE:MITOCHONDRIAL TISSUE AND H9C2 CELL, DILUTION:1:1000

Zhang T,Chen Y,Cai J,Pan M,Sun Q,Zhang J,Sun C.SOCS2 Inhibits Mitochondrial Fatty Acid Oxidation via Suppressing LepR/JAK2/AMPK Signaling Pathway in Mouse Adipocytes.Oxid Med Cell Longev.2020 Jul 13;2020:3742542.doi:10.1155/2020/3742542.PMID:32733634;PMCI
Species: Mouse
M03529-5 usage in article: APP:IF, SAMPLE:ADIPOCYTES, DILUTION:1:100

Pioglitazone improves mitochondrial function in the remnant kidney and protects against renal fibrosis in 5/6 nephrectomized rats

Neuroprotective Effect of Tea Polyphenols on Oxyhemoglobin Induced Subarachnoid Hemorrhage in Mice

Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells

pH-sensitive micelles for the intracellular co-delivery of curcumin and Pluronic L61 unimers for synergistic reversal effect of multidrug resistance

Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells

Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway

Methacryloxylethyl Cetyl Ammonium Chloride Induces DNA Damage and Apoptosis in Human Dental Pulp Cells via Generation of Oxidative Stress

N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced Intrinsic Mitochondrial Apoptosis In Vitro in Human Dental Pulp Cells

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16 Customer Q&As for Anti-Cytochrome C CYCS Antibody Picoband™ (monoclonal, 15F10)

Question

I was wanting to use your anti-Cytochrome C antibody (monoclonal, 15F10) for IHC for mouse kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse kidney identification?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

You can see on the product datasheet, M03529-5 anti-Cytochrome C antibody (monoclonal, 15F10) has been tested for Flow Cytometry, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-24

Question

We ordered your anti-Cytochrome C antibody (monoclonal, 15F10) for WB on erythroleukemia a few months ago. I am using rat, and I plan to use the antibody for Flow Cytometry next. I was wanting to use examining erythroleukemia as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

I looked at the website and datasheets of our anti-Cytochrome C antibody (monoclonal, 15F10) and it appears that M03529-5 has been tested on rat in both WB and Flow Cytometry. Thus M03529-5 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-14

Question

We are currently using anti-Cytochrome C antibody (monoclonal, 15F10) M03529-5 for rat tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-08

Answer

The anti-Cytochrome C antibody (monoclonal, 15F10) (M03529-5) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-08

Question

We were well pleased with the WB result of your anti-Cytochrome C antibody (monoclonal, 15F10). However we have observed positive staining in cerebellum mitochondrion intermembrane space. using this antibody. Is that expected? Could you tell me where is CYCS supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

From literature, cerebellum does express CYCS. Generally CYCS expresses in mitochondrion intermembrane space. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2020-03-31

Question

Would M03529-5 anti-Cytochrome C antibody (monoclonal, 15F10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

It shows on the product datasheet, M03529-5 anti-Cytochrome C antibody (monoclonal, 15F10) as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-01

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Cytochrome C antibody (monoclonal, 15F10) M03529-5. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-17

Question

Please see the WB image, lot number and protocol we used for kidney using anti-Cytochrome C antibody (monoclonal, 15F10) M03529-5. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-19

Question

We have been able to see staining in human skeletal muscle. Do you have any suggestions? Is anti-Cytochrome C antibody (monoclonal, 15F10) supposed to stain skeletal muscle positively?

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

From literature skeletal muscle does express CYCS. From Uniprot.org, CYCS is expressed in heart, cerebellum, amygdala, bone marrow, brain, kidney, lung, skeletal muscle, skin, rc testis urinary bladder, erythroleukemia, liver, among other tissues. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-08-27

Question

Is there a BSA free version of anti-Cytochrome C antibody (monoclonal, 15F10) M03529-5 available?

H. Johnson

Verified customer

Asked: 2019-04-22

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Cytochrome C antibody (monoclonal, 15F10) M03529-5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-04-22

Question

Can you help my question with product M03529-5, anti-Cytochrome C antibody (monoclonal, 15F10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Wu

Verified customer

Asked: 2018-12-12

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03529-5 anti-Cytochrome C antibody (monoclonal, 15F10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-12-12

Question

I see that the anti-Cytochrome C antibody (monoclonal, 15F10) M03529-5 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-01-22

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-01-22

Question

I would like to test anti-Cytochrome C antibody (monoclonal, 15F10) M03529-5 on mouse kidney for research purposes, then I may be interested in using anti-Cytochrome C antibody (monoclonal, 15F10) M03529-5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-08-17

Answer

The products we sell, including anti-Cytochrome C antibody (monoclonal, 15F10) M03529-5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-08-17

Question

Is this M03529-5 anti-Cytochrome C antibody (monoclonal, 15F10) reactive to the isotypes of CYCS?

E. Jha

Verified customer

Asked: 2016-02-09

Answer

The immunogen of M03529-5 anti-Cytochrome C antibody (monoclonal, 15F10) is E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-02-09

Question

Is a blocking peptide available for product anti-Cytochrome C antibody (monoclonal, 15F10) (M03529-5)?

R. Moore

Verified customer

Asked: 2015-05-22

Answer

We do provide the blocking peptide for product anti-Cytochrome C antibody (monoclonal, 15F10) (M03529-5). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-05-22

Question

Does anti-Cytochrome C antibody (monoclonal, 15F10) M03529-5 work for IHC with kidney?

J. Zhao

Verified customer

Asked: 2015-02-09

Answer

According to the expression profile of kidney, CYCS is highly expressed in kidney. So, it is likely that anti-Cytochrome C antibody (monoclonal, 15F10) M03529-5 will work for IHC with kidney.

Boster Scientific Support

Answered: 2015-02-09

Question

I am interested in using your anti-Cytochrome C antibody (monoclonal, 15F10) for respiratory electron transport studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.

S. Dhar

Verified customer

Asked: 2013-03-15

Answer

We appreciate your inquiry. This M03529-5 anti-Cytochrome C antibody (monoclonal, 15F10) is validated on a431 cells, mouse heart tissue, k562 whole cell lysate, human hela, lung cancer tissue, tissue lysate, intestinal cancer tissue, mammary cancer tissue, hela whole cell lysate, hepg2 whole cell lysate, rat heart tissue, brain tissue, kidney tissue, testis tissue. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-03-15

Order DetailsPrice
M03529-5

100μg

$370
M03529-5-10ug

10μg sample (liquid)

$99
M03529-5-Biotin

100 μg Biotin conjugated

$570
M03529-5-Cy3

100 μg Cy3 conjugated

$570
M03529-5-Dylight488

100 μg Dylight488 conjugated

$570
M03529-5-Dylight550

100 μg Dylight550 conjugated

$570
M03529-5-Dylight594

100 μg Dylight594 conjugated

$570
M03529-5-FITC

100 μg FITC conjugated

$570
M03529-5-HRP

100 μg HRP conjugated

$570
M03529-5-APC

100 μg APC conjugated

$670
M03529-5-PE

100 μg PE conjugated

$670
M03529-5-carrier-free

Carrier Free

$370

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M03529-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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