Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90)


SKU MA1026
Size 100μg/vial
Reactivity Human, Rat
Clonality Monoclonal
Clone Number CY-90
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90)
SKU/Catalog Number MA1026
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Cytokeratin Peptide 18, keratin 18 (KRT18) detection. Tested with WB, IHC-P, IHC-F in Human; rat. No cross reactivity with other proteins.
Cite This Product Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1026)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.
Reactivity Human, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, rat, By Heat
Immunohistochemistry(Frozen Section), 0.5-2μg/ml, Human, rat, -
Western blot, 0.5-1μg/ml, Human, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, Western blotting
WB: MCF-7 Cell Lysate

Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, IHC(P)
IHC(P): Human Rectal Cancer Tissue

Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q5BJY9
Gene Name KRT18
Protein Name Keratin, type I cytoskeletal 18
Tissue Specificity Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .
Alternative Names Keratin, type I cytoskeletal 18;Cytokeratin-18;CK-18;Keratin-18;K18;Krt18 ;Krt1-18 ;
Subcellular Localization Nucleus matrix . Nucleus, nucleolus . Cytoplasm .
Molecular Weight 47761 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. .
Research Areas Human, Rat

*You can search these to find other products in these research areas.
Background Intermediate filaments(IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Monoclonal clone# G2 antibody for Cytokeratin 18/KRT18 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Cytokeratin 18/KRT18 information: Molecular Weight: 47761 MW; Subcellular Localization: Nucleus matrix . Nucleus, nucleolus . Cytoplasm ; Tissue Specificity: Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue.
Write Your Own Review
Only registered users can write reviews. Please Sign in or create an account
In stock
Order Product
MA1026
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

troubleshooting_box_image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Publications

Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor %u03B3%u03B4 are involved in bacterial clearance during Salmonella enterica serovar Typhimurium %u2026
miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
The Paracrine Effect of Transplanted Human Amniotic Epithelial Cells on Ovarian Function Improvement in a Mouse Model of Chemotherapy-Induced Primary Ovarian Insufficiency
Comparison of the effect of recombinant bovine wild and mutant lipopolysaccharide-binding protein in lipopolysaccharide-challenged bovine mammary epithelial cells
Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage
Zhang, Y., Zhang, L., Yu, C.F., Du, X.Y., Liu, X.R., Liu, J.Z.,…, & Cao, B.Y. (2017). Effects of interferon tau on endometrial epithelial cells in caprine in vitro. Gene Expression Patterns. Advance online publication. doi: 10.1016/j.gep.2017.06.010
Hu J, Zhou Z, Shi S, Zhu X, Wang X, Zhang W, Hu S, Qian H, Xu W. Oncol Lett. 2013 Jan;5(1):179-184. Epub 2012 Oct 30. Mesenchymal Stem-Like Cells Isolated From Human Esophageal Carcinoma And Adjacent Non-Cancerous Tissues.
Xu B,, Jin X,, Min L, Li Q,, Deng L,, Wu H, Lin G, Chen L, Zhang H, Li C, Wang L, Zhu J,, Wang W,, Chu F,, Shen J,, Li H, Mao J,. Oncotarget. 2015 Feb 10;6(4):2434-50. Chloride Channel-3 Promotes Tumor Metastasis By Regulating Membrane Ruffling An...

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to ck18 antibody, cytokeratin 18 antibody, cytokeratin-18 antibody, keratin 18 antibody, keratin-18 antibody
Write a review for MA1026