Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)

Boster Bio Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) catalog # MA1026. Tested in IHC, WB applications. This antibody reacts with Human, Rat. Cited in 9 publication(s).

Product Info Summary

SKU: MA1026
Size: 100μg/vial
Reactive Species: Human, Rat
Host: Mouse
Application: IHC, WB

Product Name

Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)

See all Krt18 products

SKU/Catalog Number

MA1026

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) catalog # MA1026. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1026)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

CY-90

Isotype

Mouse IgG1

Immunogen

The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1026 is reactive to KRT18 in Human, Rat

Applications

MA1026 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, rat, -
Western blot, 0.5-1μg/ml, Human, rat

Validation Images & Assay Conditions

Gene/Protein Information For KRT18 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q5BJY9

Gene ID

294853

Gene Name

KRT18

Full Name

Keratin, type I cytoskeletal 18

Weight

47.761kDa

Superfamily

intermediate filament family

Alternative Names

Cell proliferation-inducing gene 46 protein; cell proliferation-inducing protein 46; CK-18; CYK18; Cytokeratin 18; cytokeratin-18; K18; Keratin 18; keratin, type I cytoskeletal 18; keratin-18; KRT18 Krt18|Krt1-18|keratin 18|keratin, type I cytoskeletal 18|CK-18|K18|cytokeratin-18|keratin 18, type I|keratin complex 1, acidic, gene 18

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on KRT18, check out the KRT18 Infographic

KRT18 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for KRT18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1026 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wei Z,Wang J,Wang Y,Wang C,Liu X,Han Z,Fu Y,Yang Z.Effects of Neutrophil Extracellular Traps on Bovine Mammary Epithelial Cells in vitro.Front Immunol.2019 May 17;10:1003.doi:10.3389/fimmu.2019.01003.PMID:31156617;PMCID:PMC6533846.
Species: Cows
MA1026 usage in article: APP:IF, SAMPLE:BMECS, DILUTION:NA

Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor %u03B3%u03B4 are involved in bacterial clearance during Salmonella enterica serovar Typhimurium %u2026

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

The Paracrine Effect of Transplanted Human Amniotic Epithelial Cells on Ovarian Function Improvement in a Mouse Model of Chemotherapy-Induced Primary Ovarian Insufficiency

Comparison of the effect of recombinant bovine wild and mutant lipopolysaccharide-binding protein in lipopolysaccharide-challenged bovine mammary epithelial cells

Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage

Zhang, Y., Zhang, L., Yu, C.F., Du, X.Y., Liu, X.R., Liu, J.Z.,…, & Cao, B.Y. (2017). Effects of interferon tau on endometrial epithelial cells in caprine in vitro. Gene Expression Patterns. Advance online publication. doi: 10.1016/j.gep.2017.06.010

Hu J, Zhou Z, Shi S, Zhu X, Wang X, Zhang W, Hu S, Qian H, Xu W. Oncol Lett. 2013 Jan;5(1):179-184. Epub 2012 Oct 30. Mesenchymal Stem-Like Cells Isolated From Human Esophageal Carcinoma And Adjacent Non-Cancerous Tissues.

Xu B,, Jin X,, Min L, Li Q,, Deng L,, Wu H, Lin G, Chen L, Zhang H, Li C, Wang L, Zhu J,, Wang W,, Chu F,, Shen J,, Li H, Mao J,. Oncotarget. 2015 Feb 10;6(4):2434-50. Chloride Channel-3 Promotes Tumor Metastasis By Regulating Membrane Ruffling An...

Have you used Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

17 Customer Q&As for Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)

Question

Will MA1026 anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-08

Answer

As indicated on the product datasheet, MA1026 anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-08

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) MA1026. Let me know if you need anything else.

P. Gonzalez

Verified customer

Asked: 2020-02-06

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-06

Question

Is a blocking peptide available for product anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) (MA1026)?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

We do provide the blocking peptide for product anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) (MA1026). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-30

Question

Would anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) MA1026 work for IHC with pancreas?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

According to the expression profile of pancreas, KRT18 is highly expressed in pancreas. So, it is likely that anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) MA1026 will work for IHC with pancreas.

Boster Scientific Support

Answered: 2019-11-19

Question

We are currently using anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) MA1026 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

The anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) (MA1026) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-03

Question

Is this MA1026 anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) reactive to the isotypes of KRT18?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

The immunogen of MA1026 anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) is The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-20

Question

We have observed staining in rat colon carcinoma. What should we do? Is anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) supposed to stain colon carcinoma positively?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

From what I have seen in literature colon carcinoma does express KRT18. From what I have seen in Uniprot.org, KRT18 is expressed in adenohypophysis, placenta, cervix, colon, pancreas, placenta uterus, vulva, liver, colon carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have KRT18 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 17924679, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Colon, Pancreas, Placenta, and Uterus, Pubmed ID: 15489334
Colon carcinoma, Pubmed ID: 9150948, 24129315
Liver, Pubmed ID: 2422083, 24275569
Placenta, Pubmed ID: 2434380
Vulva, Pubmed ID: 2434381

Boster Scientific Support

Answered: 2019-05-17

Question

Do you have a BSA free version of anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) MA1026 available?

C. Lewis

Verified customer

Asked: 2019-05-07

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) MA1026 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-07

Question

I am interested in using your anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) for extrinsic apoptotic signaling pathway studies. Has this antibody been tested with western blotting on rectal cancer tissue? We would like to see some validation images before ordering.

J. Jha

Verified customer

Asked: 2019-04-08

Answer

We appreciate your inquiry. This MA1026 anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) is validated on mammary cancer tissue, rectal cancer tissue. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-04-08

Question

Could you please confirm if this ab MA1026 stain the cleaved CK18 or the intact CK18?

Verified Customer

Verified customer

Asked: 2017-12-08

Answer

MA1026 stains the cleaved CK18 or the intact CK18.

Boster Scientific Support

Answered: 2017-12-08

Question

My colleagues were content with the WB result of your anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90). However we have been able to see positive staining in cervix carcinoma perinuclear region. nucleus, using this antibody. Is that expected? Could you tell me where is KRT18 supposed to be expressed?

W. Li

Verified customer

Asked: 2017-08-04

Answer

From literature, cervix carcinoma does express KRT18. Generally KRT18 expresses in cytoplasm, perinuclear region. nucleus,. Regarding which tissues have KRT18 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 17924679, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Colon, Pancreas, Placenta, and Uterus, Pubmed ID: 15489334
Colon carcinoma, Pubmed ID: 9150948, 24129315
Liver, Pubmed ID: 2422083, 24275569
Placenta, Pubmed ID: 2434380
Vulva, Pubmed ID: 2434381

Boster Scientific Support

Answered: 2017-08-04

Question

I was wanting to use your anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) for IHC for human pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas identification?

Verified Customer

Verified customer

Asked: 2017-08-02

Answer

As indicated on the product datasheet, MA1026 anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) has been validated for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-02

Question

Can you help my question with product MA1026, anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-05-12

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1026 anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-05-12

Question

I see that the anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) MA1026 works with IHC, what is the protocol used to produce the result images on the product page?

C. Collins

Verified customer

Asked: 2017-03-24

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-03-24

Question

We ordered your anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) for IHC on adenohypophysis in a previous experiment. I am using human, and We intend to use the antibody for WB next. I would like examining adenohypophysis as well as colon carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

E. Yang

Verified customer

Asked: 2014-04-22

Answer

I have checked the website and datasheets of our anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) and it appears that MA1026 has been validated on human in both IHC and WB. Thus MA1026 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-04-22

Question

I am interested in to test anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) MA1026 on human pancreas for research purposes, then I may be interested in using anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) MA1026 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

R. Banerjee

Verified customer

Asked: 2013-12-04

Answer

The products we sell, including anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) MA1026, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-12-04

Question

See attached the WB image, lot number and protocol we used for pancreas using anti-Cytokeratin Peptide 18 antibody (Monoclonal, CY-90) MA1026. Please let me know if you require anything else.

D. Carter

Verified customer

Asked: 2013-06-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-06-14

Size

Total: $280

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
MA1026
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.