SKU MA1026
Size 100μg/vial
Reactivity Human, Rat
Clonality Monoclonal
Clone Number CY-90
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90)
SKU/Catalog Number MA1026
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Cytokeratin Peptide 18, keratin 18 (KRT18) detection. Tested with WB, IHC-P, IHC-F in Human; rat. No cross reactivity with other proteins.
Cite This Product Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1026)
Host Mouse
Immunogen The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.
Reactivity Human, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, rat, By Heat
Immunohistochemistry(Frozen Section), 0.5-2μg/ml, Human, rat, -
Western blot, 0.5-1μg/ml, Human, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/ma1026 1 WB anti cytokeratin peptide 18 antibody monoclonal cy 90.jpg

Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, Western blotting
WB: MCF-7 Cell Lysate

/antibody/ma1026 2 IHC anti cytokeratin peptide 18 antibody monoclonal cy 90.jpg

Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, IHC(P)
IHC(P): Human Mammary Cancer Tissue

/antibody/ma1026 3 IHC anti cytokeratin peptide 18 antibody monoclonal cy 90.jpg

Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, IHC(P)
IHC(P): Human Rectal Cancer Tissue

/antibody/ma1026 4 IHC anti cytokeratin peptide 18 antibody monoclonal cy 90.jpg

Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q5BJY9
Gene Name Krt18
Protein Name Keratin, type I cytoskeletal 18
Tissue Specificity Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .
Alternative Names Keratin, type I cytoskeletal 18;Cytokeratin-18;CK-18;Keratin-18;K18;Krt18 ;Krt1-18 ;
Subcellular Localization Nucleus matrix . Nucleus, nucleolus . Cytoplasm .
Molecular Weight 47761 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. .
Background Intermediate filaments(IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Order Product (MA1026)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Publications

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
Comparison of the effect of recombinant bovine wild and mutant lipopolysaccharide-binding protein in lipopolysaccharide-challenged bovine mammary epithelial cells
The Paracrine Effect of Transplanted Human Amniotic Epithelial Cells on Ovarian Function Improvement in a Mouse Model of Chemotherapy-Induced Primary Ovarian Insufficiency
Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival
Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage
Mesenchymal stem-like cells isolated from human esophageal carcinoma and adjacent non-cancerous tissues
Zhang, Y., Zhang, L., Yu, C.F., Du, X.Y., Liu, X.R., Liu, J.Z.,…, & Cao, B.Y. (2017). Effects of interferon tau on endometrial epithelial cells in caprine in vitro. Gene Expression Patterns. Advance online publication. doi: 10.1016/j.gep.2017.06.010

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to ck18 antibody, cytokeratin 18 antibody, cytokeratin-18 antibody, keratin 18 antibody, keratin-18 antibody