|Ig Isotype||Mouse IgG1|
|Product Name||Anti-Cytokeratin Peptide 7 Antibody (Monoclonal, LDS-68)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Cytokeratin Peptide 7, keratin 7 (KRT7) detection. Tested with WB, IHC-P, IHC-F in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cytokeratin Peptide 7 Antibody (Monoclonal, LDS-68) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1024)|
|Immunogen||Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.|
Assay Dilutions Overview
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human, -
Western blot, 1-2μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
Protein Target Info (Source: Uniprot.org)
|Protein Name||Keratin, type II cytoskeletal 7|
|Tissue Specificity||KRT7: Expressed in cultured epidermal, bronchial andmesothelial cells but absent in colon, ectocervix and liver.Observed throughout the glandular cells in the junction betweenstomach and esophagus but is absent in the esophagus.|
|Alternative Names||Keratin, type II cytoskeletal 7;Cytokeratin-7;CK-7;Keratin-7;K7;Type-II keratin Kb7;Krt7 ;Kb7 , Krt2-7 ;|
|Subcellular Localization||KRT7: Cytoplasm|
|Molecular Weight||50709 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. .|
|Background||KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.|
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