Anti-Cytokeratin Peptide 7 Antibody (Monoclonal, LDS-68)

SKU MA1024
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number LDS-68
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Cytokeratin Peptide 7 Antibody (Monoclonal, LDS-68)
SKU/Catalog Number MA1024
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Cytokeratin Peptide 7, keratin 7 (KRT7) detection. Tested with WB, IHC-P, IHC-F in Human. No cross reactivity with other proteins.
Cite This Product Anti-Cytokeratin Peptide 7 Antibody (Monoclonal, LDS-68) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1024)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, By Heat
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human, -
Western blot, 1-2μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

Boster Kit Box

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q6IG12
Gene Name KRT7
Protein Name Keratin, type II cytoskeletal 7
Tissue Specificity KRT7: Expressed in cultured epidermal, bronchial andmesothelial cells but absent in colon, ectocervix and liver.Observed throughout the glandular cells in the junction betweenstomach and esophagus but is absent in the esophagus.
Alternative Names Keratin, type II cytoskeletal 7;Cytokeratin-7;CK-7;Keratin-7;K7;Type-II keratin Kb7;Krt7 ;Kb7 , Krt2-7 ;
Subcellular Localization KRT7: Cytoplasm
Molecular Weight 50709 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. .
Research Areas Human

*You can search these to find other products in these research areas.
Background KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Monoclonal clone# G2 antibody for CYTOKERATIN 7/KRT7 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CYTOKERATIN 7/KRT7 information: Molecular Weight: 50709 MW; Subcellular Localization: KRT7: Cytoplasm; Tissue Specificity: KRT7: Expressed in cultured epidermal, bronchial andmesothelial cells but absent in colon, ectocervix and liver.Observed throughout the glandular cells in the junction betweenstomach and esophagus but is absent in the esophagus.
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In stock
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MA1024
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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Publications

EPHB4 regulates human trophoblast cell line HTR-8/SVneo function: implications for the role of EPHB4 in preeclampsia
Bu S, Zhang Q, Wang Q, Lai D. Int J Oncol. 2017 Nov;51(5):1405-1414. doi: 10.3892/ijo.2017.4123. Epub 2017 Sep 14. Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF-β1-mediated cell cycle arrest

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to scl antibody, ck7 antibody, cytokeratin 7 antibody, cytokeratin-7 antibody, keratin 7 antibody, keratin-7 antibody
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