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Product Info Summary
| SKU: | PB9286 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Mucin 5AC/MUC5AC Antibody
SKU/Catalog Number
PB9286
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mucin 5AC/MUC5AC Antibody catalog # PB9286. Tested in IHC applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mucin 5AC/MUC5AC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9286)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Mucin 5AC recombinant protein (Position: D4848-H5030).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9286 is reactive to MUC5AC in Human, Rat
Calculated molecular weight
585.6 kDa
Background of MUC5AC
Mucin-5AC is a protein that in humans is encoded by the MUC5AC gene. It is mapped to 11p15.5. MUC5AC is highly expressed in adult trachea and stomach. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease (COPD). It has been showed that activation of the NF-kappa-B signaling pathway by inflammatory cytokine TNF-alpha upregulated MUC5AC mRNA expression nearly 6-fold. MUC5AC is also a direct and critical mediator of resistance during intestinal nematode infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9286 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Positive Control
IHC: human gastric carcinoma tissue, rat stomach tissue
Validation Images & Assay Conditions
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IHC analysis of Mucin-5AC using anti-Mucin-5AC antibody (PB9286).
Mucin-5AC was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mucin-5AC Antibody (PB9286) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Mucin-5AC using anti-Mucin-5AC antibody (PB9286).
Mucin-5AC was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mucin-5AC Antibody (PB9286) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Mucin 5AC/MUC5AC Antibody (PB9286)
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Customer Reviews
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5 Customer Q&As for Anti-Mucin 5AC/MUC5AC Antibody
Question
We ordered your anti-Mucin 5AC/MUC5AC antibody for WB on stomach a few years ago. I am using human, and I plan to use the antibody for IHC next. I am interested in examining stomach as well as nasal cavity epithelium in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
I took a look at the website and datasheets of our anti-Mucin 5AC/MUC5AC antibody and it seems that PB9286 has been validated on human in both WB and IHC. Thus PB9286 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-29
Question
you antibody using your anti-Mucin 5AC/MUC5AC antibody for o-linked glycosylation of mucins studies. Has this antibody been tested with western blotting on gastric cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
We appreciate your inquiry. This PB9286 anti-Mucin 5AC/MUC5AC antibody is tested on gastric cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-14
Question
We have been able to see staining in human trachea. Do you have any suggestions? Is anti-Mucin 5AC/MUC5AC antibody supposed to stain trachea positively?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
From what I have seen in literature trachea does express MUC5AC. From what I have seen in Uniprot.org, MUC5AC is expressed in nasal cavity epithelium, trachea, tracheobronchial mucosa, placenta trachea, nasal polyp, colon, stomach, among other tissues. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675
Boster Scientific Support
Answered: 2019-07-16
Question
We are currently using anti-Mucin 5AC/MUC5AC antibody PB9286 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The anti-Mucin 5AC/MUC5AC antibody (PB9286) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-14
Question
Our team were satisfied with the WB result of your anti-Mucin 5AC/MUC5AC antibody. However we have been able to see positive staining in colon secreted. using this antibody. Is that expected? Could you tell me where is MUC5AC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
According to literature, colon does express MUC5AC. Generally MUC5AC expresses in secreted. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675
Boster Scientific Support
Answered: 2019-05-23
