This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
MUC5AC antibody (PB9286) performs well in IHC and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
IHC analysis of Mucin-5AC using anti-Mucin-5AC antibody (PB9286).
Mucin-5AC was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mucin-5AC Antibody (PB9286) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Mucin-5AC was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mucin-5AC Antibody (PB9286) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-Mucin 5AC/MUC5AC Antibody
MUC5AC antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | IHC |
Tested Applications
MUC5AC antibody was positively detected by IHC in:
human gastric carcinoma tissue, rat stomach tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Mucin 5AC/MUC5AC Antibody
View all MUC5AC Antibodies
View all MUC5AC Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Mucin 5AC recombinant protein (Position: D4848-H5030).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
585.6 kDa
Background of MUC5AC
Mucin-5AC is a protein that in humans is encoded by the MUC5AC gene. It is mapped to 11p15.5. MUC5AC is highly expressed in adult trachea and stomach. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease (COPD). It has been showed that activation of the NF-kappa-B signaling pathway by inflammatory cytokine TNF-alpha upregulated MUC5AC mRNA expression nearly 6-fold. MUC5AC is also a direct and critical mediator of resistance during intestinal nematode infection.
Cite This Product
Anti-Mucin 5AC/MUC5AC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9286)
Protocols
Immunohistochemistry (IHC) protocol for MUC5AC antibody
Publications
Application:
Species:
Neutrophil elastase induces MUC5AC secretion via protease-activated receptor 2
Published: 2013-02-08
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY
Application: ELISA
Impact Factor: 2.33
Species: Human
PMID: 23392769
Review
Have you used Anti-Mucin 5AC/MUC5AC Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Mucin 5AC/MUC5AC Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9286
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026