Anti-Mucin 5AC/MUC5AC Antibody Picoband™

MUC5AC antibody

Boster Bio Anti-Mucin 5AC/MUC5AC Antibody Picoband™ catalog # PB9286. Tested in IHC applications. This antibody reacts with Human, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PB9286
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: IHC

Customers Who Bought This Also Bought

Product Name

Anti-Mucin 5AC/MUC5AC Antibody Picoband™

View all MUC5AC Antibodies

SKU/Catalog Number

PB9286

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mucin 5AC/MUC5AC Antibody Picoband™ catalog # PB9286. Tested in IHC applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mucin 5AC/MUC5AC Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9286)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Mucin 5AC recombinant protein (Position: D4848-H5030).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9286 is reactive to MUC5AC in Human, Rat

Applications

PB9286 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

585 kDa

Calculated molecular weight

585.57kDa

Background of MUC5AC

Mucin-5AC is a protein that in humans is encoded by the MUC5AC gene. It is mapped to 11p15.5. MUC5AC is highly expressed in adult trachea and stomach. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease (COPD). It has been showed that activation of the NF-kappa-B signaling pathway by inflammatory cytokine TNF-alpha upregulated MUC5AC mRNA expression nearly 6-fold. MUC5AC is also a direct and critical mediator of resistance during intestinal nematode infection.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For MUC5AC (Source: Uniprot.org, NCBI)

Gene Name

MUC5AC

Full Name

Mucin-5AC

Weight

585.57kDa

Alternative Names

gastric mucin; leB; lewis B blood group antigen; major airway glycoprotein; MUC5; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin 5AC, oligomeric mucus/gel-forming; mucin-5 subtype AC, tracheobronchial; mucin-5AC; TBM; tracheobronchial mucin MUC5AC MUC5, TBM, leB, mucin mucin 5AC, oligomeric mucus/gel-forming mucin-5AC|gastric mucin|lewis B blood group antigen|major airway glycoprotein|mucin 5, subtypes A and C, tracheobronchial/gastric|mucin 5AC, oligomeric mucus/gel-forming pseudogene|mucin-5 subtype AC, tracheobronchial|tracheobronchial mucin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MUC5AC, check out the MUC5AC Infographic

MUC5AC infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MUC5AC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9286 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Huang J,Liu J,Chang G,Wang Y,Ma N,Roy AC,Shen X.Glutamine Supplementation Attenuates the Inflammation Caused by LPS-Induced Acute Lung Injury in Mice by Regulating the TLR4/MAPK Signaling Pathway.Inflammation.2021 Jun 23.doi:10.1007/s10753-021-01491-2.Epub ahead of print.PMID:34160729.
Species: Mouse
PB9286 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:1000

Zhou J, Perelman Jm, Kolosov Vp, Zhou X. Mol Cell Biochem. 2013 May;377(1-2):75-85. Doi: 10.1007/S11010-013-1572-3. Epub 2013 Feb 8. Neutrophil Elastase Induces Muc5Ac Secretion Via Protease-Activated Receptor 2.

Have you used Anti-Mucin 5AC/MUC5AC Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Mucin 5AC/MUC5AC Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

5 Customer Q&As for Anti-Mucin 5AC/MUC5AC Antibody Picoband™

Question

We ordered your anti-Mucin 5AC/MUC5AC antibody for WB on stomach a few years ago. I am using human, and I plan to use the antibody for IHC next. I am interested in examining stomach as well as nasal cavity epithelium in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

I took a look at the website and datasheets of our anti-Mucin 5AC/MUC5AC antibody and it seems that PB9286 has been validated on human in both WB and IHC. Thus PB9286 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-29

Question

you antibody using your anti-Mucin 5AC/MUC5AC antibody for o-linked glycosylation of mucins studies. Has this antibody been tested with western blotting on gastric cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

We appreciate your inquiry. This PB9286 anti-Mucin 5AC/MUC5AC antibody is tested on gastric cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-14

Question

We have been able to see staining in human trachea. Do you have any suggestions? Is anti-Mucin 5AC/MUC5AC antibody supposed to stain trachea positively?

Verified Customer

Verified customer

Asked: 2019-07-16

Answer

From what I have seen in literature trachea does express MUC5AC. From what I have seen in Uniprot.org, MUC5AC is expressed in nasal cavity epithelium, trachea, tracheobronchial mucosa, placenta trachea, nasal polyp, colon, stomach, among other tissues. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675

Boster Scientific Support

Answered: 2019-07-16

Question

We are currently using anti-Mucin 5AC/MUC5AC antibody PB9286 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

The anti-Mucin 5AC/MUC5AC antibody (PB9286) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-14

Question

Our team were satisfied with the WB result of your anti-Mucin 5AC/MUC5AC antibody. However we have been able to see positive staining in colon secreted. using this antibody. Is that expected? Could you tell me where is MUC5AC supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

According to literature, colon does express MUC5AC. Generally MUC5AC expresses in secreted. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675

Boster Scientific Support

Answered: 2019-05-23

Order DetailsPrice
PB9286

100μg

$370
PB9286-10ug

10μg sample (liquid)

$99
PB9286-Biotin

100 μg Biotin conjugated

$570
PB9286-Cy3

100 μg Cy3 conjugated

$570
PB9286-Dylight488

100 μg Dylight488 conjugated

$570
PB9286-Dylight550

100 μg Dylight550 conjugated

$570
PB9286-Dylight594

100 μg Dylight594 conjugated

$570
PB9286-FITC

100 μg FITC conjugated

$570
PB9286-HRP

100 μg HRP conjugated

$570
PB9286-APC

100 μg APC conjugated

$670
PB9286-PE

100 μg PE conjugated

$670
PB9286-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
PB9286
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.