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Product Info Summary
| SKU: | A00751-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-DDX3 DDX3X Antibody
SKU/Catalog Number
A00751-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DDX3 DDX3X Antibody (Catalog # A00751-3). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DDX3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DDX3 DDX3X Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00751-3)
Host
Rabbit
Contents
DDX3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DDX3 antibody was raised against a 16 amino acid synthetic peptide from near the center of human DDX3. The immunogen is located within amino acids 460 - 510 of DDX3.
Cross-reactivity
DDX3 antibody will detect both DDX3 and DBY.
Reactive Species
A00751-3 is reactive to DDX3X in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
73.2 kDa
Background of DDX3X
DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00751-3 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DDX3 antibody can be used for the detection of DDX3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 μg/mL.
Specific Publications For Anti-DDX3 DDX3X Antibody (A00751-3)
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