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- Table of Contents
Facts about ATP-dependent RNA helicase DDX3X.
Is involved in a number of steps of gene expression, such as transcription, mRNA maturation, mRNA export and translation. However, the specific mechanisms are not known and some functions may be specific for a subset of mRNAs.
Human | |
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Gene Name: | DDX3X |
Uniprot: | O00571 |
Entrez: | 1654 |
Belongs to: |
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DEAD box helicase family |
DBXCAP-Rf; DDX14; DDX3DEAD/H box-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked; DEAD box protein 3, X-chromosomal; DEAD box, X isoform; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; EC 3.6.1; EC 3.6.4.13; helicase like protein 2; Helicase-like protein 2; HLP2ATP-dependent RNA helicase DDX3X
Mass (kDA):
73.243 kDA
Human | |
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Location: | Xp11.4 |
Sequence: | X; NC_000023.11 (41333308..41364472) |
Cell membrane. Nucleus. Cytoplasm. Cytoplasm, Stress granule. Inflammasome. Cell projection, lamellipodium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Shuttles between the nucleus and the cytosol (PubMed:15507209, PubMed:18636090, PubMed:29899501). Exported from the nucleus partly through the XPO1/CRM1 system and partly through NXF1/TAP (PubMed:15507209, PubMed:18636090, PubMed:18596238). Localizes to nuclear pores on the outer side of the nuclear membrane (PubMed:15507209). In the cytosol, partly colocalizes with mitochondria (PubMed:20127681). At G0, predominantly lo
You might be wondering how you can use DDX3's the DDX3X Marker for your job, but you may not know how to use it correctly. This article will give an outline of DDX3 3X marker and its applications. Additionally, you will be informed about Boster Bio products, and their uses. This article is general in nature and is applicable to all scientists around the world.
The DDX3 DDX3X marker is found in a variety of tissues, including breast. The protein is believed to play an important role in tumorigenesis as well as the development of cancer. Studies have demonstrated that DDX3 is expressed in cancerous cells of the colorectal, and plays both tumor suppressor and oncogenic roles. DDX3X elimination led to a reduction in cell proliferation in MCF7-derived breast cancer cells. It also decreased the expression of KLF4, which is a cell cycle repressor. A high Gleason score is also associated with DDX3 expression.
DDX3X is a protein that regulates the translation of target mRNAs. It is found in intergenic, 5'-UTR and 3'-UTR regions. DDX3X is also present in mature mRNAs. The protein was also expressed in the 5'-UTR, and the transcription start codon was highlighted with an arrowhead of red.
In addition to controlling HCV replication, DDX3X also controls the expression of G3BP1. The expression of DDX3X in human cells is increased in patients with hepatitis C and hepatitis B virus infection. Additionally, it increases the production of IFN-b, which inhibits the replication of HCV. These proteins are also linked to the formation of SGs in cells that are stressed.
The DDX3 DDX3X molecular marker recognizes specific features in the RNA. It recognizes a large number of targets for mRNA, including those involved in RNA metabolism, translation, and cell processes. Its target list is extensive, and is represented by genes that are involved in translation, chromosome organization and trafficking in the cellular.
The expression of DDX3X can be detected in different types of cancer. It is detected in certain types of esophageal, breast, cervical, and endocervical tumors. However, DDX3X expression is lower in certain types of melanoma may be lower. For instance the DDX3X marker is less prominent in prostate and the thymoma.
Additionally, DDX3X expression has been associated with poorer outcomes in liver, breast and pancreatic cancers. DDX3X expression has been found to be linked with lymph node metastasis. This is an indicator of poor survival. It was also detected in a study on pancreatic ductal carcinoma. This study showed a significant correlation with survival data. The results of DDX3 expression are also reported in a variety of studies involving DDX3Y.
In prostate cancer there is cells in the cytoplasm DDX3X expression has been linked with the status of metastasis for the cancerous cells. Specific shRNA-mediated DDX3X inhibition reduced breast cancer metastasis in vivo. DDX3X can also regulate Rac1 mRNA expression, and stabilize b-catenin proteins levels.
The mutation of DDX3X is associated with poor prognosis as well as progression in pediatric MB. Additionally, the loss of this gene is associated with chemotherapy resistance in the aggressive non-Hodgkin's lymphoma subtypes. Two types of DDX3X variants are identified. The two first mutations are located on the Y chromosome and the third is located on the X.
DDX3X protein is present in MB tumors after transfection. Mutations in DDX3X can cause green tumors, while red tumors have the wild-type form. Each group is comprised of three replicas. The paired samples were immunolabeled using 35S-Met/Cys in order to identify the protein contained within the cells. The Western Blot shows the different levels of DDX3X in the replicas that were pooled. As an indicator of loading, the similar bactin protein was employed.
A poor prognosis may be associated with metastatic expression of DDX3X for breast cancer. Patients who had low DDX3X level had a poorer prognosis and were more likely to develop distant metastasis. Also, knockdown of DDX3 increased colon cancer cell migration and promoted metastasis in the vivo. In addition, DDX3 knockdown enhanced expression of E-cadherin and Snail. These results suggest that DDX3 could be a target for therapeutic treatment for breast cancer.
Mutations of DDX3X cause cancer cells to undergo an cellular stress response which causes global translation impairment. Mutations of DDX3X hinder eEF2K function, which grants survival to MB tumor cells. Mutations in DDX3X may inhibit translation and promote hyper-assembly SGs during metabolic adaption. This causes tumor cells to expand faster but they remain intolerant to DMARDs.
The DDX3 or DDX3X Antibody is intended to be used for research purposes. It is not suitable for therapeutic or diagnostic applications. The antibody is available through a variety of suppliers. Contact your supplier for more details. The antiserum was affinity purified with a rabbit epitope specific immunogen. It is a secure and effective way to study DDX3 protein and its associated signaling pathway.
DDX3X Antibody is available in a variety of formats. Antibodies against DDX3 can be used to perform immunohistochemistry or Western blot. Other applications include immunofluorescence as well as flow Cytometry. Chimpanzees and zebrafish have orthologs to the human protein. It is available from more than 30 different suppliers. This antibody can be used to analyze the regulation of numerous biological processes.
DDX3 is a multifunctional member of the DEAD family of RNA Helicases. The conserved motif in DEAD box proteins enables them to perform multiple steps in gene expression. It is required for the HIV-1 nuclear export transcripts. DDX3 can also function as an anti-tumor agent. It reduces tumor cell colony growth and boosts the levels of the cdk inhibitor p21 Waf1/Cip1.
PMID: 10329544 by Owsianka A.M., et al. Hepatitis C virus core protein interacts with a human DEAD box protein DDX3.
PMID: 9381176 by Lahn B.T., et al. Functional coherence of the human Y chromosome.