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Breast Cancer Regulation
Rabbit Polyclonal DKK1 Antibody. Validated in WB and tested in Human, Mouse, Rat.
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Our Boster Quality Guarantee for Anti-DKK1 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
The following reagents are used to generate the images below for Anti-DKK1 Antibody (PA1462).
Figure 1. Western blot analysis of DKK1 using anti-DKK1 antibody (PA1462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: rat spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK1 antigen affinity purified polyclonal antibody (Catalog # PA1462) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK1 at approximately 39KD. The expected band size for DKK1 is at 29KD.
*if product is indicated to react with multiple species, protein info is based on the human gene.
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