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Product Info Summary
| SKU: | PA1462 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Dickkopf-related protein 1 DKK1 Antibody Picoband®
SKU/Catalog Number
PA1462
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Dickkopf-related protein 1 DKK1 Antibody catalog # PA1462. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dickkopf-related protein 1 DKK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1462)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human DKK1, different from the related mouse and rat sequences by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1462 is reactive to DKK1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
28.7 kDa
Background of DKK1
DKK1, Dickkopf-related protein 1, is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. By genomic sequence analysis, determined that the DKK1 gene contains 4 exons. The DKK1 gene is mapped to 10q11.2 using FISH. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1462 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human placenta tissue, human A431 whole cell, human Hela whole cell, human PC-3 whole cell, human Caco-2 whole cell, human A549 whole cell, rat spleen tissue, mouse thymus tissue
Validation Images & Assay Conditions
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Western blot analysis of DKK1 using anti-DKK1 antibody (PA1462).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: rat spleen tissue lysates,
Lane 8: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK1 antigen affinity purified polyclonal antibody (Catalog # PA1462) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK1 at approximately 39KD. The expected band size for DKK1 is at 29KD.
Specific Publications For Anti-Dickkopf-related protein 1 DKK1 Antibody Picoband® (PA1462)
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5 Customer Q&As for Anti-Dickkopf-related protein 1 DKK1 Antibody Picoband®
Question
Can you provide information about the crystal structures of PA1462?
Verified customer
Asked: 2021-01-15
Answer
Unfortunately, we do not have crystal structures available for the Anti-Dickkopf-Related Protein 1 DKK1 Antibody (PA1462).
Boster Scientific Support
Answered: 2021-01-19
Question
you antibody using your anti-DKK1 antibody for positive regulation of cell death studies. Has this antibody been tested with western blotting on a431 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
I appreciate your inquiry. This PA1462 anti-DKK1 antibody is tested on human placenta tissue, a431 whole cell lysates, hela whole cell lysates, a549 whole cell lysates, rat spleen tissue, mouse thymus tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-03
Question
We have seen staining in human fetal kidney. Any tips? Is anti-DKK1 antibody supposed to stain fetal kidney positively?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
Based on literature fetal kidney does express DKK1. Based on Uniprot.org, DKK1 is expressed in decidua, leiomyosarcoma, fetal kidney, cerebellum, brain, among other tissues. Regarding which tissues have DKK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Fetal kidney, Pubmed ID: 10570958
Leiomyosarcoma, Pubmed ID: 10383463
Boster Scientific Support
Answered: 2019-06-06
Question
My team were satisfied with the WB result of your anti-DKK1 antibody. However we have observed positive staining in fetal kidney secreted. using this antibody. Is that expected? Could you tell me where is DKK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
From literature, fetal kidney does express DKK1. Generally DKK1 expresses in secreted. Regarding which tissues have DKK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Fetal kidney, Pubmed ID: 10570958
Leiomyosarcoma, Pubmed ID: 10383463
Boster Scientific Support
Answered: 2019-03-07
Question
We are currently using anti-DKK1 antibody PA1462 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-30
Answer
The anti-DKK1 antibody (PA1462) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-30
