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Product Info Summary
| SKU: | PA1810 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Transcription factor E2F2 E2F2 Antibody Picoband®
SKU/Catalog Number
PA1810
BA0810-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Transcription factor E2F2 E2F2 Antibody catalog # PA1810. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor E2F2 E2F2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1810)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human E2F2, identical to the related mouse sequence.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1810 is reactive to E2F2 in Human, Mouse, Rat
Observed Molecular Weight
48 kDa
Calculated molecular weight
47.5 kDa
Background of E2F2
E2F2 (E2F transcription factor 2) also called E2F-2, is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F2 gene is mapped to 1p36 by fluorescence in situ hybridization. Electrophoretic mobility shift assays revealed the specific binding of E2F2 to ECE1b promoter sequences containing either allele of the C-338A polymorphism, with the -338A allele being associated with an increased affinity to E2F2 compared with -338C. The ability of Myc to induce S phase was impaired in the absence of either E2f2 or E2f3 but not E2f1 or E2f4. In contrast, the ability of Myc to induce apoptosis was markedly reduced in cells deleted for E2f1 but not E2f2 or E2f3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1810 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of E2F2 using anti-E2F2 antibody (PA1810).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F2 antigen affinity purified polyclonal antibody (PA1810) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for E2F2 at approximately 48 kDa. The expected band size for E2F2 is at 48 kDa.
Specific Publications For Anti-Transcription factor E2F2 E2F2 Antibody Picoband® (PA1810)
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Customer Q&As
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4 Customer Q&As for Anti-Transcription factor E2F2 E2F2 Antibody Picoband®
Question
Is there a BSA free version of anti-E2F2 antibody PA1810 available?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-E2F2 antibody PA1810 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-18
Question
Does anti-E2F2 antibody PA1810 work on dog WB with liver?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Our lab technicians have not validated anti-E2F2 antibody PA1810 on dog. You can run a BLAST between dog and the immunogen sequence of anti-E2F2 antibody PA1810 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-18
Question
We are currently using anti-E2F2 antibody PA1810 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
R. Singh
Verified customer
Asked: 2017-11-24
Answer
The anti-E2F2 antibody (PA1810) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-24
Question
I was wanting to use your anti-E2F2 antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
M. Li
Verified customer
Asked: 2014-08-20
Answer
As indicated on the product datasheet, PA1810 anti-E2F2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-20
