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Product Info Summary
| SKU: | A00353-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Granzyme B/Gzmb Antibody Picoband®
SKU/Catalog Number
A00353-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Granzyme B/Gzmb Antibody Picoband® catalog # A00353-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Granzyme B/Gzmb Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00353-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Granzyme B recombinant protein (Position: I21-S247).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00353-1 is reactive to Gzmb in Mouse, Rat
Observed Molecular Weight
28 kDa
Calculated molecular weight
27.5 kDa
Background of Gzmb
Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00353-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse thymus tissue, mouse liver tissue
Validation Images & Assay Conditions
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Western blot analysis of Granzyme B using anti-Granzyme B antibody (A00353-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Granzyme B antigen affinity purified polyclonal antibody (Catalog # A00353-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Granzyme B at approximately 28KD. The expected band size for Granzyme B is at 28KD.
Specific Publications For Anti-Granzyme B/Gzmb Antibody Picoband® (A00353-1)
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Customer Q&As
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2 Customer Q&As for Anti-Granzyme B/Gzmb Antibody Picoband®
Question
Could you tell me the concentration of the product Anti-Granzyme B/Gzmb Antibody?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
The concentration of the Anti-Granzyme B/Gzmb Antibody is 0.0825 mg/ml.
Boster Scientific Support
Answered: 2018-01-29
Question
I would like to know the recognition part of this Anti-Granzyme B/Gzmb Antibody. It is stated that the immunogen was synthetic peptide (Human), do you know which part of the Granzyme b this peptide stands? Is it in the N-terminal, C-Terminal or the middle part?
Verified Customer
Verified customer
Asked: 2014-10-31
Answer
The immunogen of the product Anti-Granzyme B/Gzmb Antibody is from the N-terminal region of the human Granzyme B.
Boster Scientific Support
Answered: 2014-10-31
