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Product Info Summary
|Reactive Species:||Mouse, Rat|
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Anti-Granzyme B/Gzmb Antibody Picoband™
Boster Bio Anti-Granzyme B/Gzmb Antibody Picoband™ catalog # A00353-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Granzyme B/Gzmb Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00353-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived mouse Granzyme B recombinant protein (Position: I21-S247).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00353-1 is reactive to Gzmb in Mouse, Rat
A00353-1 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Granzyme B using anti-Granzyme B antibody (A00353-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Granzyme B antigen affinity purified polyclonal antibody (Catalog # A00353-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Granzyme B at approximately 28KD. The expected band size for Granzyme B is at 28KD.
Gene/Protein Information For Gzmb (Source: Uniprot.Org, NCBI)
peptidase S1 family
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT Gzmb|AI553453, CCP, CCP-1/C1, CCP-1/C11, CCP1, Ctl, Ctla, Ctla-1, Ctla1, GZB|granzyme B|granzyme B(G,H)|cytotoxic cell protease 1|fragmentin-2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Gzmb, check out the Gzmb Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Gzmb: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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