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Product Info Summary
| SKU: | EK0405 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
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Product info
Product Name
Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™
SKU/Catalog Number
EK0405
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il4 in cell culture supernatants and serum. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0405)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: H21-S140
Sensitivity
<1 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0405 is reactive to Il4 in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0405 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0405-CAP | Anti-Mouse Il4 Pre-coated 96-well strip microplate | 1 |
| EK0405-ST | Mouse Il4 Standard | 2 vials, 10 ng/tube |
| EK0405-DA | Mouse Il4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.056 | 0.097 | 0.131 | 0.212 | 0.374 | 0.813 | 1.489 | 2.084 |
Data Example Images
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Mouse IL-4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-4, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Some articles we found to cite concentrations of IL-4 in samples: 22304769 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 15 | 96 | 280 | 16 | 93 | 275 |
| Standard deviation | 1.11 | 6.62 | 20.44 | 1.48 | 6.69 | 20.9 |
| CV (%) | 7.4% | 6.9% | 7.3% | 9.3% | 7.2% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 15 | 14 | 13 | 16 | 14 | 1.11 | 7.9% |
| Sample 2 | 96 | 90 | 93 | 94 | 93 | 2.16 | 2.3% |
| Sample 3 | 280 | 285 | 256 | 248 | 267 | 15.61 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For Il4 (Source: Uniprot.Org, NCBI)
Uniprot ID
P07750
Gene ID
16189
Gene Name
Il4
Full Name
Interleukin-4
Weight
15.834kDa
Superfamily
IL-4/IL-13 family
Alternative Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra Il4|BSF-1, Il, Il-4|interleukin 4|interleukin-4|B-cell IgG differentiation factor|B-cell growth factor 1|B-cell stimulatory factor 1|IGG1 induction factor|lymphocyte stimulatory factor 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il4, check out the Il4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™ (EK0405)
EK0405 has been cited in 37 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Qiu-yue Fang,Shu-ping Chen,Jun-qiao Wang,Xiao-jun Huang,Qi-xing Nie,Glyn O. Philips,Steve W. Cui,Ya-jing Li,Shao-ping Nie,Fractions from natural Cordyceps sinensis alleviated intestinal injury in Cyclophosphamide-induced mice,Bioactive Carbohydrates and Dietary Fibre,2021,100271,ISSN 2212-6198,https://doi.org/10.1016/j.bcdf.2021.100271.
Species: Mouse
EK0405 usage in article: APP:ELISA, SAMPLE:SMALL INTESTINE, DILUTION:NA
Ze-Hui Liu,Hui-Ling Xu,Guang-Wei Han,Li-Na Tao,Ying Lu,Su-Ya Zheng,Wei-Huan Fang,Fang He,A self-assembling nanoparticle: Implications for the development of thermostable vaccine candidates,International Journal of Biological Macromolecules,2021,,ISSN 0141-8130,https://doi.org/10.1016/j.ijbiomac.2021.06.024.
Species: Mouse
EK0405 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Cui H,Cheng Y,He Y,Cheng W,Zhao W,Zhao H,Zhou FH,Wang L,Dong J,Cai S.The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model.Mol Med Rep.2020 Nov;22(5):3723-3734.doi:10.3892/mmr.2020.11450.Epub 2020 Aug 21.PMID:33000187;PMCID:PMC7533517.
Species: Mouse
EK0405 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION: NA
Zhu Y,Liang H,Liu X,Wu J,Yang C,Wong TM, Kwan KYH,Cheung KMC,Wu S,Yeung KWK.Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration. Sci Adv.2021 Apr 2;7(14):eabf6654.doi:10. 1126/sciadv.abf6654.PMID:33811079.
Species: Mouse,Rat
EK0405 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Xi Y,Ma Y,Xie B,Di A,Xu S,Luo X,Wang C,Dai H,Yan G,Qi Z.Vitamin D3 combined with antibody agents suppresses alloreactive memory T-cell responses to induce heart allograft long-term survival.Transpl Immunol.2021 Feb 13:101374.doi:10.1016/j.trim.2021.101374
Species: Mouse
EK0405 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Zhang Z,Song X,Zhang Z,Li H,Duan Y,Zhang H,Lu H,Luo C, Wang M.The molecular characterization and immune protection of adhesion protein 65 (AP65) of Trichomonas vaginalis.Microb Pathog.2021 Jan 20:104750.doi:10.1016/j.micpath.2021.104750.Epub ahead of prin
Species: Mouse,Rat
EK0405 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Zhou Z,Hou J,Mo Y,Ren M,Yang G,Qu Z,Hu Y.Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice.Eur J Pharmacol.2020 Feb 15;869:1
Species: Mouse
Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding?Toxoplasma gondii?elongation factor 1-alpha
Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis
5-BDBD ameliorates an OVA-induced allergic asthma by the reduction of Th2 cytokines production
Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice
Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1?) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis
Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration
DNA vaccination with a gene encoding?Toxoplasma gondii?Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice
Hybrid phage displaying SLAQVKYTSASSI induces protection against?Candida albicans?challenge in BALB/c mice
Molecular Adjuvant Ag85A Enhances Protection against Influenza A Virus in Mice Following DNA Vaccination
Yang N, Zhang H, Cai X, Shang Y. Int J Mol Med. 2018 Feb;41(2):818-828. doi: 10.3892/ijmm.2017.3292. Epub 2017 Nov 28. Epigallocatechin-3-gallate inhibits inflammation and epithelial-mesenchymal transition through the PI3K/AKT pathway via upregula...
Han, X.P., Zhang, F.Q., Tan, X.S., Liu, L., Ma, W.X., Ou-Yang, H.F., & Wu, C.G. (2017). EPO modified MSCs can inhibit asthmatic airway remodeling in an animal model. Journal of Cellular Biochemistry. Advance online publication. doi: 10.1002/jcb.26268
Li W, Han D, Liang S, Zhong Z, Li X, Wen J, Lin H, Wang L, Li X, Zhong X, Zhong F. Reprod Toxicol. 2014 Jun;45:31-8. Doi: 10.1016/J.Reprotox.2014.01.005. Epub 2014 Jan 17. Molecular Mechanism For The Effects Of E. Coli Heat-Labile Enterotoxin On M...
Li D, Hua S, Fan Y, Xu S, Duan X, Liu L, Che Y, Li S, Tan Y. Immunol Lett. 2011 Mar 30;135(1-2):124-8. Doi: 10.1016/J.Imlet.2010.10.012. Epub 2010 Nov 10. Dna Vaccine Expressing Repeated Carcinoembryonic Antigen (Cea)(625-667) Induces Strong Immun...
Zhang Y, Liang S, Li X, Wang L, Zhang J, Xu J, Huo S, Cao X, Zhong Z, Zhong F. Vaccine. 2015 Jul 9;33(30):3480-7. Doi: 10.1016/J.Vaccine.2015.05.068. Epub 2015 Jun 9. Mutual Enhancement Of Il-2 And Il-7 On Dna Vaccine Immunogenicity Mainly Involve...
Li J, Li Ff, Zuo W, Zhou Y, Hao Hy, Dang J, Jiang M, He Mz, Deng Dr. J Huazhong Univ Sci Technolog Med Sci. 2014 Aug;34(4):586-90. Doi: 10.1007/S11596-014-1320-2. Epub 2014 Aug 19. Up-Regulated Expression Of Tim-3/Gal-9 At Maternal-Fetal Interface...
Huang L, Cao J. Oxid Med Cell Longev. 2014;2014:248786. Doi: 10.1155/2014/248786. Epub 2014 Mar 5. The Protective Effects Of Shen-Fu Injection On Experimental Acute Pancreatitis In A Rat Model.
Ou J, Shi W, Xu Y, Tao Z. Ann Allergy Asthma Immunol. 2014 Dec;113(6):658-665.E1. Doi: 10.1016/J.Anai.2014.08.015. Epub 2014 Sep 17. Intranasal Immunization With Dna Vaccine Coexpressing Der P 1 And Ubiquitin In An Allergic Rhinitis Mouse Model.
Jin Q, Cheng J, Liu Y, Wu J, Wang X, Wei S, Zhou X, Qin Z, Jia J, Zhen X. Brain Behav Immun. 2014 Aug;40:131-42. Doi: 10.1016/J.Bbi.2014.03.003. Epub 2014 Mar 12. Improvement Of Functional Recovery By Chronic Metformin Treatment Is Associated With...
Qiu Jl, Zhu Wl, Lu Yj, Bai Zf, Liu Zg, Zhao P, Sun C, Zhang Yb, Li H, Liu W. Neurochem Int. 2015 Jun-Jul;85-86:46-52. Doi: 10.1016/J.Neuint.2015.04.007. Epub 2015 May 4. The Selective Mglur5 Agonist Chpg Attenuates So2-Induced Oxidative Stress And...
Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert...
Wang Xj, Deng Hz, Jiang B, Yao H. Int J Colorectal Dis. 2012 May;27(5):575-81. Doi: 10.1007/S00384-011-1352-Z. Epub 2011 Nov 15. The Natural Plant Product Sophocarpine Ameliorates Dextran Sodium Sulfate-Induced Colitis In Mice By Regulating Cytoki...
Huang J, Wang P, Li M, Ge J, Chen J, Chen X. Int J Mol Med. 2015 Aug;36(2):493-500. Doi: 10.3892/Ijmm.2015.2249. Epub 2015 Jun 16. Trichostatin A Reduces Cisplatin-Induced Ototoxicity Through The Stat6 Signaling Pathway.
Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...
Li S, Hu X, Wang Z, Wu M, Zhang J. Inflamm Res. 2015 May;64(5):363-71. Doi: 10.1007/S00011-015-0816-Y. Epub 2015 Mar 27. Different Profiles Of Notch Signaling In Cigarette Smoke-Induced Pulmonary Emphysema And Bleomycin-Induced Pulmonary Fibrosis.
Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Eur J Pharmacol. 2012 Jun 15;685(1-3):186-97. Doi: 10.1016/J.Ejphar.2012.04.012. Epub 2012 Apr 20. Stephanthraniline A Inhibits The Proliferation And Activation Of T Cells In Vitro And In Vivo.
Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Int Immunopharmacol. 2010 Oct;10(10):1153-60. Doi: 10.1016/J.Intimp.2010.06.021. Epub 2010 Jul 4. Comparison Of Immunosuppressive Activity Of Stephanoside E And Its Aglycone From Stephanotis Mucronata In Vitro.
Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????
Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu...
Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...
Li L, Yu Ch, Ying Hz, Yu Jm. J Ethnopharmacol. 2013 May 2;147(1):238-44. Doi: 10.1016/J.Jep.2013.03.012. Epub 2013 Mar 15. Antiviral Effects Of Modified Dingchuan Decoction Against Respiratory Syncytial Virus Infection In Vitro And In An Immunosup...
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Customer Q&As
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6 Customer Q&As for Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
K. Anand
Verified customer
Asked: 2020-09-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-09-27
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-4 using the EK0405 Mouse IL-4 Picokine® ELISA Kit?
B. Banerjee
Verified customer
Asked: 2020-06-06
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-06-06
Question
Q: What is the optimal O.D. value for IL-4 ELISA kit? I used your IL-4 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-4 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-4 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-4 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2019-01-02
Question
I purchased your product Mouse IL-4 ELISA Kit PicoKine and it is a monoclonal antibody. Could you tell me if it is raised against the protein when it is processed in the cell or when it is already secreted by the cell?
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
The product datasheets of Mouse I L-4 ELISA Kit PicoKine indicate that the immunogen was a recombinant full length mouse protein and has been tested and characterize in different applications such Flow cytometry, IHC-Fr, Western blot and ELISA.
Boster Scientific Support
Answered: 2018-10-11
Question
Q: how to proceed with the analysis of ELISA data? I have obtained IL-4 level in serum.
J. Wu
Verified customer
Asked: 2015-12-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-12-09
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2015-02-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2015-02-24
