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Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™
Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il4 in cell culture supernatants and serum.. Sensitivity: 1pg/ml. Cited in 35 publication(s).
Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™ Product Summary
| SKU: | EK0405 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactivity: | Mouse |
| Sample Type: | cell culture supernatants and serum. |
| Sample Volume: | 100μl per well |
| Sensitivity: | <1 pg/ml |
| Assay Range: | 7.8 pg/ml - 500 pg/ml |
Overview
| Product Name | Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™
See more IL-4 products |
|---|---|
| Catalog# | EK0405 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) |
| Description | Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il4 in cell culture supernatants and serum.. Sensitivity: 1pg/ml. |
| Cite This Product | Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0405) |
| ELISA Validation | ELISA Validation Information |
Product Specs
| Reactivity/Species | Mouse |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernatants and serum. |
| Sensitivity | <1 pg/ml |
| Assay Range | 7.8 pg/ml - 500 pg/ml |
| Immunogen/Standard | Expression system for standard: E.coli; Immunogen sequence: H21-S140 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Il4 (Source: Uniprot.org, NCBI)
| Uniprot Id | P07750 |
|---|---|
| NCBI Gene Id | 16189 |
| Species Of This Entry | Mouse |
| Gene Name | Il4 |
| Protein Name | Interleukin-4 |
| Superfamily | IL-4/IL-13 family |
| Alternative Names | IL-4|B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra |
| Molecular Weight | 15834 |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il4, check out the Il4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Il4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Kit Components And Assay QC Data Details Of Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0405-CAP | Anti-Mouse Il4 Pre-coated 96-well strip microplate | 1 |
| EK0405-ST | Mouse Il4 Standard | 2 vials, 10 ng/tube |
| EK0405-DA | Mouse Il4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0405 Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.056 | 0.097 | 0.131 | 0.212 | 0.374 | 0.813 | 1.489 | 2.284 |
We measured random samples of Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 15 | 96 | 280 | 16 | 93 | 275 |
| Standard deviation | 1.11 | 6.62 | 20.44 | 1.48 | 6.69 | 20.9 |
| CV (%) | 7.4% | 6.9% | 7.3% | 9.3% | 7.2% | 7.6% |
We measured the performance consistency of Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 15 | 14 | 13 | 16 | 14 | 1.11 | 7.9% |
| Sample 2 | 96 | 90 | 93 | 94 | 93 | 2.16 | 2.3% |
| Sample 3 | 280 | 285 | 256 | 248 | 267 | 15.61 | 5.8% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 35
| Cui H,Cheng Y,He Y,Cheng W,Zhao W,Zhao H,Zhou FH,Wang L,Dong J,Cai S.The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model.Mol Med Rep.2020 Nov;22(5):3723-3734.doi:10.3892/mmr.2020.11450.Epub 2020 Aug 21.PMID:33000187;PMCID:PMC7533517. Species: Mouse EK0405 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION: NA |
PubMed ID 33000187 |
| Zhu Y,Liang H,Liu X,Wu J,Yang C,Wong TM, Kwan KYH,Cheung KMC,Wu S,Yeung KWK.Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration. Sci Adv.2021 Apr 2;7(14):eabf6654.doi:10. 1126/sciadv.abf6654.PMID:33811079. Species: Mouse,Rat EK0405 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA |
PubMed ID 33811079 |
| Xi Y,Ma Y,Xie B,Di A,Xu S,Luo X,Wang C,Dai H,Yan G,Qi Z.Vitamin D3 combined with antibody agents suppresses alloreactive memory T-cell responses to induce heart allograft long-term survival.Transpl Immunol.2021 Feb 13:101374.doi:10.1016/j.trim.2021.101374 Species: Mouse EK0405 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
PubMed ID 33592299 |
| Zhang Z,Song X,Zhang Z,Li H,Duan Y,Zhang H,Lu H,Luo C, Wang M.The molecular characterization and immune protection of adhesion protein 65 (AP65) of Trichomonas vaginalis.Microb Pathog.2021 Jan 20:104750.doi:10.1016/j.micpath.2021.104750.Epub ahead of prin Species: Mouse,Rat EK0405 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
PubMed ID 33484808 |
| Zhou Z,Hou J,Mo Y,Ren M,Yang G,Qu Z,Hu Y.Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice.Eur J Pharmacol.2020 Feb 15;869:1 Species: Mouse |
PubMed ID 31837991 |
| Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding?Toxoplasma gondii?elongation factor 1-alpha | PubMed ID 26497908 |
| Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis | PubMed ID 29867820 |
| 5-BDBD ameliorates an OVA-induced allergic asthma by the reduction of Th2 cytokines production | PubMed ID 29796218 |
| Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice | PubMed ID 28633646 |
| Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1?) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis | PubMed ID 28243226 |
| Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration | PubMed ID 27411313 |
| DNA vaccination with a gene encoding?Toxoplasma gondii?Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice | PubMed ID 25201636 |
| Hybrid phage displaying SLAQVKYTSASSI induces protection against?Candida albicans?challenge in BALB/c mice | PubMed ID 24448202 |
| Molecular Adjuvant Ag85A Enhances Protection against Influenza A Virus in Mice Following DNA Vaccination | PubMed ID 23223215 |
| Yang N, Zhang H, Cai X, Shang Y. Int J Mol Med. 2018 Feb;41(2):818-828. doi: 10.3892/ijmm.2017.3292. Epub 2017 Nov 28. Epigallocatechin-3-gallate inhibits inflammation and epithelial-mesenchymal transition through the PI3K/AKT pathway via upregula... | PubMed ID 29207033 |
| Han, X.P., Zhang, F.Q., Tan, X.S., Liu, L., Ma, W.X., Ou-Yang, H.F., & Wu, C.G. (2017). EPO modified MSCs can inhibit asthmatic airway remodeling in an animal model. Journal of Cellular Biochemistry. Advance online publication. doi: 10.1002/jcb.26268 | PubMed ID 28686347 |
| Li W, Han D, Liang S, Zhong Z, Li X, Wen J, Lin H, Wang L, Li X, Zhong X, Zhong F. Reprod Toxicol. 2014 Jun;45:31-8. Doi: 10.1016/J.Reprotox.2014.01.005. Epub 2014 Jan 17. Molecular Mechanism For The Effects Of E. Coli Heat-Labile Enterotoxin On M... | PubMed ID 24440567 |
| Li D, Hua S, Fan Y, Xu S, Duan X, Liu L, Che Y, Li S, Tan Y. Immunol Lett. 2011 Mar 30;135(1-2):124-8. Doi: 10.1016/J.Imlet.2010.10.012. Epub 2010 Nov 10. Dna Vaccine Expressing Repeated Carcinoembryonic Antigen (Cea)(625-667) Induces Strong Immun... | PubMed ID 21073899 |
| Zhang Y, Liang S, Li X, Wang L, Zhang J, Xu J, Huo S, Cao X, Zhong Z, Zhong F. Vaccine. 2015 Jul 9;33(30):3480-7. Doi: 10.1016/J.Vaccine.2015.05.068. Epub 2015 Jun 9. Mutual Enhancement Of Il-2 And Il-7 On Dna Vaccine Immunogenicity Mainly Involve... | PubMed ID 26055295 |
| Li J, Li Ff, Zuo W, Zhou Y, Hao Hy, Dang J, Jiang M, He Mz, Deng Dr. J Huazhong Univ Sci Technolog Med Sci. 2014 Aug;34(4):586-90. Doi: 10.1007/S11596-014-1320-2. Epub 2014 Aug 19. Up-Regulated Expression Of Tim-3/Gal-9 At Maternal-Fetal Interface... | PubMed ID 25135732 |
| Huang L, Cao J. Oxid Med Cell Longev. 2014;2014:248786. Doi: 10.1155/2014/248786. Epub 2014 Mar 5. The Protective Effects Of Shen-Fu Injection On Experimental Acute Pancreatitis In A Rat Model. | PubMed ID 24738018 |
| Ou J, Shi W, Xu Y, Tao Z. Ann Allergy Asthma Immunol. 2014 Dec;113(6):658-665.E1. Doi: 10.1016/J.Anai.2014.08.015. Epub 2014 Sep 17. Intranasal Immunization With Dna Vaccine Coexpressing Der P 1 And Ubiquitin In An Allergic Rhinitis Mouse Model. | PubMed ID 25240330 |
| Jin Q, Cheng J, Liu Y, Wu J, Wang X, Wei S, Zhou X, Qin Z, Jia J, Zhen X. Brain Behav Immun. 2014 Aug;40:131-42. Doi: 10.1016/J.Bbi.2014.03.003. Epub 2014 Mar 12. Improvement Of Functional Recovery By Chronic Metformin Treatment Is Associated With... | PubMed ID 24632338 |
| Qiu Jl, Zhu Wl, Lu Yj, Bai Zf, Liu Zg, Zhao P, Sun C, Zhang Yb, Li H, Liu W. Neurochem Int. 2015 Jun-Jul;85-86:46-52. Doi: 10.1016/J.Neuint.2015.04.007. Epub 2015 May 4. The Selective Mglur5 Agonist Chpg Attenuates So2-Induced Oxidative Stress And... | PubMed ID 25953665 |
| Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert... | PubMed ID 24368037 |
| Wang Xj, Deng Hz, Jiang B, Yao H. Int J Colorectal Dis. 2012 May;27(5):575-81. Doi: 10.1007/S00384-011-1352-Z. Epub 2011 Nov 15. The Natural Plant Product Sophocarpine Ameliorates Dextran Sodium Sulfate-Induced Colitis In Mice By Regulating Cytoki... | PubMed ID 22083209 |
| Huang J, Wang P, Li M, Ge J, Chen J, Chen X. Int J Mol Med. 2015 Aug;36(2):493-500. Doi: 10.3892/Ijmm.2015.2249. Epub 2015 Jun 16. Trichostatin A Reduces Cisplatin-Induced Ototoxicity Through The Stat6 Signaling Pathway. | PubMed ID 26080623 |
| Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The... | PubMed ID 25744647 |
| Li S, Hu X, Wang Z, Wu M, Zhang J. Inflamm Res. 2015 May;64(5):363-71. Doi: 10.1007/S00011-015-0816-Y. Epub 2015 Mar 27. Different Profiles Of Notch Signaling In Cigarette Smoke-Induced Pulmonary Emphysema And Bleomycin-Induced Pulmonary Fibrosis. | PubMed ID 25813392 |
| Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Eur J Pharmacol. 2012 Jun 15;685(1-3):186-97. Doi: 10.1016/J.Ejphar.2012.04.012. Epub 2012 Apr 20. Stephanthraniline A Inhibits The Proliferation And Activation Of T Cells In Vitro And In Vivo. | PubMed ID 22543083 |
| Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Int Immunopharmacol. 2010 Oct;10(10):1153-60. Doi: 10.1016/J.Intimp.2010.06.021. Epub 2010 Jul 4. Comparison Of Immunosuppressive Activity Of Stephanoside E And Its Aglycone From Stephanotis Mucronata In Vitro. | PubMed ID 20609400 |
| Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (???????????? | PubMed ID 25428340 |
| Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu... | PubMed ID 22464914 |
| Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ... | PubMed ID 23423691 |
| Li L, Yu Ch, Ying Hz, Yu Jm. J Ethnopharmacol. 2013 May 2;147(1):238-44. Doi: 10.1016/J.Jep.2013.03.012. Epub 2013 Mar 15. Antiviral Effects Of Modified Dingchuan Decoction Against Respiratory Syncytial Virus Infection In Vitro And In An Immunosup... | PubMed ID 23506991 |
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Questions and answers from customers related to EK0405 Mouse IL-4/Interleukin-4 ELISA Kit PicoKine™
6 Related Questions
Question
I purchased your product Mouse IL-4 ELISA Kit PicoKine and it is a monoclonal antibody. Could you tell me if it is raised against the protein when it is processed in the cell or when it is already secreted by the cell?
Verified Customer
Asked: 2018-10-11
Answer
The product datasheets of Mouse I L-4 ELISA Kit PicoKine indicate that the immunogen was a recombinant full length mouse protein and has been tested and characterize in different applications such Flow cytometry, IHC-Fr, Western blot and ELISA.
Boster Scientific Support
Answered: 2018-10-11
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
K. Anand
Asked: 2020-09-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-09-27
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-4 using the EK0405 Mouse IL-4 Picokine® ELISA Kit?
B. Banerjee
Asked: 2020-06-06
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-06-06
Question
Q: What is the optimal O.D. value for IL-4 ELISA kit? I used your IL-4 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-4 even though the O.D. values are not very high?
Verified Customer
Asked: 2019-01-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-4 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-4 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2019-01-02
Question
Q: how to proceed with the analysis of ELISA data? I have obtained IL-4 level in serum.
J. Wu
Asked: 2015-12-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-12-09
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2015-02-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2015-02-24
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