Anti-EIF2C1/AGO1 Antibody Picoband™

Boster Bio Anti-EIF2C1/AGO1 Antibody Picoband™ catalog # A00826-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00826-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-EIF2C1/AGO1 Antibody Picoband™

See all AGO1/EIF2C1 products

SKU/Catalog Number

A00826-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EIF2C1/AGO1 Antibody Picoband™ catalog # A00826-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EIF2C1/AGO1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00826-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human EIF2C1/AGO1 (376-409aa EISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGR), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

A00826-1 is reactive to AGO1 in Human, Mouse, Rat

Applications

A00826-1 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For AGO1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9UL18

Gene ID

26523

Gene Name

AGO1

Full Name

Protein argonaute-1

Weight

97.214kDa

Superfamily

argonaute family

Alternative Names

AGO1Q99; argonaute 1; argonaute1; DKFZp686M13167; eIF2C 1; eIF-2C 1; EIF2C; Eukaryotic translation initiation factor 2C 1; eukaryotic translation initiation factor 2C, 1; GERP95; Golgi Endoplasmic Reticulum protein 95 kDa; hAgo1; protein argonaute-1; Putative RNA-binding protein Q99 AGO1 EIF2C, EIF2C1, GERP95, Q99, hAgo1 argonaute RISC component 1 protein argonaute-1|Golgi Endoplasmic Reticulum protein 95 kDa|argonaute 1, RISC catalytic component|argonaute RISC catalytic component 1|argonaute1|eIF-2C 1|eIF2C 1|eukaryotic translation initiation factor 2C, 1|putative RNA-binding protein Q99

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AGO1, check out the AGO1 Infographic

AGO1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AGO1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-EIF2C1/AGO1 Antibody Picoband™

Question

Will A00826-1 anti-EIF2C1/AGO1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Brown

Verified customer

Asked: 2020-04-14

Answer

You can see on the product datasheet, A00826-1 anti-EIF2C1/AGO1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-14

Question

Is a blocking peptide available for product anti-EIF2C1/AGO1 antibody (A00826-1)?

Verified Customer

Verified customer

Asked: 2020-02-06

Answer

We do provide the blocking peptide for product anti-EIF2C1/AGO1 antibody (A00826-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-06

Question

Will anti-EIF2C1/AGO1 antibody A00826-1 work for IHC with prostate gland?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

According to the expression profile of prostate gland, AGO1 is highly expressed in prostate gland. So, it is likely that anti-EIF2C1/AGO1 antibody A00826-1 will work for IHC with prostate gland.

Boster Scientific Support

Answered: 2019-08-26

Question

I see that the anti-EIF2C1/AGO1 antibody A00826-1 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-11-24

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-11-24

Question

We are currently using anti-EIF2C1/AGO1 antibody A00826-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

W. Baker

Verified customer

Asked: 2013-05-01

Answer

The anti-EIF2C1/AGO1 antibody (A00826-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-05-01

Conjugation

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A00826-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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