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Product Info Summary
| SKU: | PA1573 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Eph receptor A1/EPHA1 Antibody Picoband®
SKU/Catalog Number
PA1573
BA0832 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Eph receptor A1/EPHA1 Antibody catalog # PA1573. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Eph receptor A1/EPHA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1573)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor A1, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1573 is reactive to EPHA1 in Human, Mouse, Rat
Observed Molecular Weight
113 kDa
Calculated molecular weight
108.1 kDa
Background of EPHA1
EPHA1, EPH receptor A1, is a protein that in humans is encoded by the EPHA1 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The EPHA1 gene is mapped to 7q34-q35. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1573 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: human Hela whole cell, human Colo320 whole cell, human HepG2 whole cell, human Caco-2 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
IHC: Rat Brain Tissue, Human Colon Cancer Tissue
Validation Images & Assay Conditions
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Western blot analysis of Eph receptor A1 using anti-Eph receptor A1 antibody (PA1573).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Colo320 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A1 antigen affinity purified polyclonal antibody (Catalog # PA1573) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A1 at approximately 113 kDa. The expected band size for Eph receptor A1 is at 108 kDa.
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Anti-Eph receptor A1 antibody, PA1573, IHC(P)
IHC(P): Rat Brain Tissue
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Anti-Eph receptor A1 antibody, PA1573, IHC(P)
IHC(P): Human Colon Cancer Tissue
Specific Publications For Anti-Eph receptor A1/EPHA1 Antibody Picoband® (PA1573)
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1 Customer Q&As for Anti-Eph receptor A1/EPHA1 Antibody Picoband®
Question
We are currently using anti-Eph receptor A1/EPHA1 antibody PA1573 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
The anti-Eph receptor A1/EPHA1 antibody (PA1573) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-31
