Anti-Eph receptor A1/EPHA1 Antibody
Rabbit IgG polyclonal antibody for Ephrin type-A receptor 1(EPHA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-Eph receptor A1/EPHA1 Antibody
See all EPHA1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for EPHA1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. EPHA1 information: Molecular Weight: 108127 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein ; Tissue Specificity: Overexpressed in several carcinomas.|
|Cite This Product||Anti-Eph receptor A1/EPHA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1573)|
|Specificity||Anti-Eph receptor A1/EPHA1 Antibody (PA1573) reacts with Human, Mouse, Rat EPHA1, in native form and recombinant. Superfamily members of EPHA1 are not reactive to PA1573.|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor A1(963-976aa HQKRILCSIQGFKD), identical to the related rat and mouse sequences.|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-Eph receptor A1/EPHA1 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-Eph receptor A1/EPHA1 Antibody (PA1573).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-Eph receptor A1 antibody, PA1573, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Intestine Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 5: U87 Cell Lysate
Lane 6: A549 Cell Lysate
Lane 7: COLO320 Cell Lysate
Lane 8: SW620 Cell Lysate
Lane 9: HELA Cell Lysate
Anti-Eph receptor A1 antibody, PA1573, IHC(P)
IHC(P): Rat Brain Tissue
Anti-Eph receptor A1 antibody, PA1573, IHC(P)
IHC(P): Human Colon Cancer Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Ephrin type-A receptor 1|
|Tissue Specificity||Overexpressed in several carcinomas.|
|Alternative Names||Ephrin type-A receptor 1;hEpha1;188.8.131.52;EPH tyrosine kinase;EPH tyrosine kinase 1;Erythropoietin-producing hepatoma receptor;Tyrosine-protein kinase receptor EPH;EPHA1;EPH, EPHT, EPHT1;|
|Subcellular Localization||Cell membrane ; Single-pass type I membrane protein .|
|Molecular Weight||108127 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis. .|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||EPHA1, EPH receptor A1, is a protein that in humans is encoded by the EPHA1 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The EPHA1 gene is mapped to 7q34-q35. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis.|
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to eph antibody, epha1 antibody