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Product Info Summary
| SKU: | EK0568 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
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Product info
Product Name
Mouse MCP-1 ELISA Kit PicoKine™
SKU/Catalog Number
EK0568
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse MCP-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl2 in cell culture supernatants and serum. Sensitivity: 6pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse MCP-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0568)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: Q24-R96
Sensitivity
<6 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0568 is reactive to Ccl2 in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0568 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0568-CAP | Anti-Mouse Ccl2 Pre-coated 96-well strip microplate | 1 |
| EK0568-ST | Mouse Ccl2 Standard | 2 vials, 10 ng/tube |
| EK0568-DA | Mouse Ccl2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.014 | 0.051 | 0.090 | 0.193 | 0.406 | 0.726 | 1.465 | 1.897 |
Data Example Images
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Mouse MCP-1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse MCP-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 39 | 224 | 394 | 40 | 231 | 398 |
| Standard deviation | 1.56 | 10.75 | 27.18 | 2 | 12.47 | 27.46 |
| CV (%) | 4% | 4.8% | 6.9% | 5% | 5.4% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Ccl2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 39 | 36 | 34 | 34 | 35 | 2.04 | 5.8% |
| Sample 2 | 224 | 228 | 228 | 219 | 224 | 3.69 | 1.6% |
| Sample 3 | 394 | 375 | 431 | 394 | 398 | 20.3 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For Ccl2 (Source: Uniprot.Org, NCBI)
Uniprot ID
P10148
Gene ID
20296
Gene Name
Ccl2
Full Name
C-C motif chemokine 2
Weight
16.326kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF Ccl2|AI323594, HC11, JE, MCA, MCAF, MCP, MCP-, MCP-1, MCP1, SMC-C, SMC-CF, Scy, Scya2, Sig, Sigje|chemokine (C-C motif) ligand 2|C-C motif chemokine 2|monocyte chemoattractant protein 1|monocyte chemotactic protein 1|platelet-derived growth factor-inducible protein JE|small-inducible cytokine A2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Ccl2, check out the Ccl2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ccl2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Mouse MCP-1 ELISA Kit PicoKine™ (EK0568)
EK0568 has been cited in 31 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yanyan Yu,Xiangqian Li,Wenpeng Hu,et al.Self-developed NF-κB inhibitor 270 protects against LPS-induced acute kidney injury and lung injury through improving inflammation.Authorea.June 22,2021.
Species: Human,Mouse
EK0568 usage in article: APP:ELISA, SAMPLE:PLASMA AND BMDMS, DILUTION:NA
Luo YY,Yang ZQ,Lin XF,Zhao FL,Tu HT,Wang LJ,Wen MY,Xian SX.Knockdown of lncRNA PVT1 attenuated macrophage M1 polarization and relieved sepsis induced myocardial injury via miR-29a/HMGB1 axis.Cytokine.2021 Apr 9:155509.doi:10.1016/j.cyto.2021.155509.Epub ahead of print.PMID:33840587.
Species: Mouse
EK0568 usage in article: APP:ELISA, SAMPLE:BMDMS, DILUTION:NA
Pingjun Zhu,Kun Wan,Ming Yin,Peng Hu,Yifan Que,Xin Zhou,Lei Zhang,Tianzhi Li,Yingzhen Du,Guogang Xu,Xiangqun Fang,"RIPK3 Induces Cardiomyocyte Necroptosis via Inhibition of AMPK-Parkin-Mitophagy in Cardiac Remodelling after Myocardial Infarction",Oxidative Medicine and Cellular Longevity,vol.2021,Article ID 6635955,11 pages,2021.https://doi.org/10.1155/2021/6635955
Species: Mouse
EK0568 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Liu Y,Qi Y.Vildagliptin, a CD26/DPP4 inhibitor, ameliorates bleomycin-induced pulmonary fibrosis via regulating the extracellular matrix.Int Immunopharmacol.2020 Oct;87:106774.doi:10.1016/j.intimp.2020.106774.Epub 2020 Jul 27. PMID:32731178.
Species: Human,Mouse
EK0568 usage in article: APP:ELISA, SAMPLE:PLASMA AND LUNG TISSUE, DILUTION:NA
Liu Y,Feng M,Cai J,Li S,Dai X,Shan G,Wu S.Repurposing bortezomib for choroidal neovascularization treatment via antagonizing VEGF-A and PDGF-D mediated signaling.Exp Eye Res.2021 Jan 18;204:108446.doi:10.1016/j.exer.2021.108446.Epub ahead of print.PMID:33
Species: Mouse
EK0568 usage in article: APP:ELISA, SAMPLE:RPE TISSUE, DILUTION:NA
Sharma D,Arora S,Banerjee A,Singh J.Improved insulin sensitivity in obese-diabetic mice via chitosan Nanomicelles mediated silencing of pro-inflammatory Adipocytokines.Nanomedicine.2021 Jan 15:102357.doi:10.1016/j.nano.2020.102357.Epub ahead of print. PMI
Species: Mouse
EK0568 usage in article: APP:ELISA, SAMPLE:ADIPOSE TISSUE, DILUTION:NA
Chen X,He Y,Zhu Y,Du J,Sun H.linc-AAM Facilitates Gene Expression Contributing to Macrophage Activation and Adaptive Immune Responses.Cell Rep.2021 Jan 5;34(1):108584.doi:10.1016/j.celrep.2020.108584.PMID:33406422.
Species: Human,Mouse
EK0568 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Wu,N,Xu,X‐F,Xin,J‐Q, et al. The effects of nuclear factor‐kappa B in pancreatic stellate cells on inflammation and fibrosis of chronic pancreatitis. J Cell Mol Med.2020; 00: 1– 15. https://doi.org/10.1111/jcmm.16213
Species: Mouse
EK0568 usage in article: APP:ELISA, SAMPLE:PSCS, DILUTION:NA
Wu X, Lin L, Wu H. Ferulic acid alleviates lipopolysaccharide-induced acute lung injury through inhibiting TLR4/NF-κB signaling pathway. J Biochem Mol Toxicol. 2020 Nov 2:e22664. doi: 10.1002/jbt.22664. Epub ahead of print. PMID: 33140555.
Species: Mouse
EK0568 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA
3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation
Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction
Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway
3-(3-Pyridylmethylidene)-2-indolinone Reduces the Severity of Colonic Injury in a Murine Model of Experimental Colitis
Yang S, Zhang J, Wang S, Zhao X, Shi J. Oncotarget. 2017 Aug 24;8(53):91185-91198. doi: 10.18632/oncotarget.20434. eCollection 2017 Oct 31. SOCS2 overexpression alleviates diabetic nephropathy in rats by inhibiting the TLR4/NF-κB pathway
Du, J., Chen, X., Wang, C., & Sun, H. (2017). Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha. Journal of Ethnopharmacology. Advance online publication. doi: 1...
Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ...
Wang Zm, Gao W, Wang H, Zhao D, Nie Zl, Shi Jq, Zhao S, Lu X, Wang Ls, Yang Zj. Cell Physiol Biochem. 2014;33(5):1349-58. Doi: 10.1159/000358702. Epub 2014 May 5. Green Tea Polyphenol Epigallocatechin-3-Gallate Inhibits Tnf-??-Induced Production O...
Xu Y, Kong X, Zhou H, Zhang X, Liu J, Yan J, Xie H, Xie Y. Thromb Res. 2014 Aug;134(2):384-92. Doi: 10.1016/J.Thromres.2014.05.017. Epub 2014 May 20. Oxldl/??2Gpi/Anti-??2Gpi Complex Induced Macrophage Differentiation To Foam Cell Involving Tlr4/N...
Meneses Gc, Lib??rio Ab, De Daher Ef, Da Silva Gb Jr, Da Costa Mf, Pontes Ma, Martins Am. Bmc Infect Dis. 2014 Aug 20;14:451. Doi: 10.1186/1471-2334-14-451. Urinary Monocyte Chemotactic Protein-1 (Mcp-1) In Leprosy Patients: Increased Risk For Kid...
Jiang Jx, Zhang Sj, Xiong Yk, Jia Yl, Sun Yh, Lin Xx, Shen Hj, Xie Qm, Yan Xf. Plos One. 2015 Jun 2;10(6):E0128278. Doi: 10.1371/Journal.Pone.0128278. Ecollection 2015. Eets Attenuate Ox-Ldl-Induced Ltb4 Production And Activity By Inhibiting P38 M...
Aizman I, Vinodkumar D, Mcgrogan M, Bates D. Stem Cells Dev. 2015 Jul 15;24(14):1623-34. Doi: 10.1089/Scd.2015.0083. Epub 2015 May 20. Cell Injury-Induced Release Of Fibroblast Growth Factor 2: Relevance To Intracerebral Mesenchymal Stromal Cell T...
Tu J, Yang L, Zhou X, Qi K, Wang J, Kouadir M, Xu L, Yin X, Zhao D. J Mol Neurosci. 2014 Jan;52(1):107-16. Doi: 10.1007/S12031-013-0140-3. Epub 2013 Nov 13. Prp106-126 And A?? 1-42 Peptides Induce Bv-2 Microglia Chemotaxis And Proliferation.
Tu J, Chen B, Yang L, Qi K, Lu J, Zhao D. J Mol Neurosci. 2015 Jun;56(2):509-18. Doi: 10.1007/S12031-015-0553-2. Epub 2015 Apr 14. Amyloid-?? Activates Microglia And Regulates Protein Expression In A Manner Similar To Prions.
Zhang R, Jiang F, Chen Cs, Wang T, Feng J, Tao T, Qin X. Mediators Inflamm. 2015;2015:956082. Doi: 10.1155/2015/956082. Epub 2015 May 31. Serum Levels Of Il-1 ?? , Il-6, Tgf- ?? , And Mmp-9 In Patients Undergoing Carotid Artery Stenting And Regula...
Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin Dc, Wang Dw. J Cell Physiol. 2015 Sep;230(9):2108-19. Doi: 10.1002/Jcp.24939. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization And Prevent Lps-Induced Cardiac Dysfunction.
Kong J, Kong L, Kong J, Ke S, Gao J, Ding X, Zheng L, Sun H, Sun W. J Transl Med. 2012 Nov 21;10:230. Doi: 10.1186/1479-5876-10-230. After Insufficient Radiofrequency Ablation, Tumor-Associated Endothelial Cells Exhibit Enhanced Angiogenesis And P...
Liu H, Yang Lx, Guo Rw, Zhu Gf, Shi Yk, Wang Xm, Qi F, Guo Cm, Ye Js, Yang Zh, Liang X. Int J Cardiol. 2013 Oct 9;168(4):3248-53. Doi: 10.1016/J.Ijcard.2013.04.141. Epub 2013 May 1. Functional Blockage Of Emmprin Ameliorates Atherosclerosis In Apo...
Lin Y, Liu Hl, Fang J, Yu Ch, Xiong Yk, Yuan K. Food Chem Toxicol. 2014 Jun;68:290-6. Doi: 10.1016/J.Fct.2014.03.026. Epub 2014 Mar 29. Anti-Fatigue And Vasoprotective Effects Of Quercetin-3-O-Gentiobiose On Oxidative Stress And Vascular Endotheli...
Jiang Jx, Zhang Sj, Liu Yn, Lin Xx, Sun Yh, Shen Hj, Yan Xf, Xie Qm. Eur J Pharmacol. 2014 Mar 15;727:43-51. Doi: 10.1016/J.Ejphar.2014.01.045. Epub 2014 Jan 31. Eets Alleviate Ox-Ldl-Induced Inflammation By Inhibiting Lox-1 Receptor Expression In...
Huang Wy, Wang J, Liu Ym, Zheng Qs, Li Cy. Eur J Pharmacol. 2014 Jan 15;723:67-72. Doi: 10.1016/J.Ejphar.2013.11.041. Epub 2013 Dec 11. Inhibitory Effect Of Malvidin On Tnf-??-Induced Inflammatory Response In Endothelial Cells.
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...
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Customer Reviews
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1 Reviews For Mouse MCP-1 ELISA Kit PicoKine™
0
"Good reproducible estimation"
Excellent
Source: Biocompare.com
"Good reproducible estimation"
--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student
| Application | ELISA |
|---|---|
| Sample | Centrifuged cell culture media supernatant |
| Detection | -- |
"Had to perform the analysis twice initially as the background wasn't clear. Overall, good reproducible estimation. Reliable, reproducible. Good product but would appreciate a faster assay kit with less chances of manual error." (Customer’s Tip: "Perform washing steps at least 6-7 times to get a clearer background with 60 seconds wait period in between each washing.")
Divya Sharma
Verified customer
Submitted 2019-05-21
Customer Q&As
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7 Customer Q&As for Mouse MCP-1 ELISA Kit PicoKine™
Question
Q: how do I analyze ELISA data? I have performed CCL2 level in serum.
Verified Customer
Verified customer
Asked: 2020-08-07
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-08-07
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-06-01
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-06-01
Question
Can you confirm if the EK0568 is compatible with ELISA microplate washers?
Verified customer
Asked: 2019-05-30
Answer
Our ELISA kits should be compatible for all ELISA microplate washers as long as it supports a 96 well plate.
Boster Scientific Support
Answered: 2019-05-30
Question
Can the sample diluent buffer, ABC diluent buffer and antibody diluent buffer be used interchangeably between the kit?
Verified customer
Asked: 2019-05-28
Answer
For the Mouse MCP-1 ELISA Kit PicoKine™ (EK0568), the sample diluent buffer, ABC diluent buffer and antibody diluent buffer can be used interchangeably between the kit. However, the sample diluent buffer can only be used to dilute standards and samples, ABC diluent buffer is used to dilute ABC only and antibody diluent buffer is used to dilute antibody only.
Boster Scientific Support
Answered: 2019-05-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
W. Wright
Verified customer
Asked: 2017-08-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-08-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
C. Young
Verified customer
Asked: 2014-08-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2014-08-26
Question
Q: if the enzyme conjugated CCL2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL2 antigen?
Verified Customer
Verified customer
Asked: 2014-05-22
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-05-22
