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Mouse MCP-1 ELISA Kit PicoKine™
Mouse MCP-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl2 in cell culture supernatants and serum.. Sensitivity: 6pg/ml. Cited in 30 publication(s). Independently reviewed in 1 review(s).
Reviews (1) See details
Mouse MCP-1 ELISA Kit PicoKine™ Product Summary
| SKU: | EK0568 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactivity: | Mouse |
| Sample Type: | cell culture supernatants and serum. |
| Sample Volume: | 100μl per well |
| Sensitivity: | <6 pg/ml |
| Assay Range: | 15.6 pg/ml - 1,000 pg/ml |
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Overview
| Product Name | Mouse MCP-1 ELISA Kit PicoKine™
See more Ccl2 products |
|---|---|
| Catalog# | EK0568 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) |
| Description | Mouse MCP-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl2 in cell culture supernatants and serum.. Sensitivity: 6pg/ml. |
| Cite This Product | Mouse MCP-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0568) |
| ELISA Validation | ELISA Validation Information |
Product Specs
| Reactivity/Species | Mouse |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernatants and serum. |
| Sensitivity | <6 pg/ml |
| Assay Range | 15.6 pg/ml - 1,000 pg/ml |
| Immunogen/Standard | Expression system for standard: E.coli; Immunogen sequence: Q24-R96 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Ccl2 (Source: Uniprot.org, NCBI)
| Uniprot Id | P10148 |
|---|---|
| NCBI Gene Id | 20296 |
| Species Of This Entry | Mouse |
| Gene Name | Ccl2 |
| Protein Name | C-C motif chemokine 2 |
| Superfamily | intercrine beta (chemokine CC) family |
| Alternative Names | Ccl2|C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF |
| Molecular Weight | 16326 |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ccl2, check out the Ccl2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Ccl2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Kit Components And Assay QC Data Details Of Mouse MCP-1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0568-CAP | Anti-Mouse Ccl2 Pre-coated 96-well strip microplate | 1 |
| EK0568-ST | Mouse Ccl2 Standard | 2 vials, 10 ng/tube |
| EK0568-DA | Mouse Ccl2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0568 Mouse MCP-1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.014 | 0.090 | 0.193 | 0.406 | 0.726 | 1.465 | 1.897 | 2.338 |
We measured random samples of Mouse MCP-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 39 | 224 | 394 | 40 | 231 | 398 |
| Standard deviation | 1.56 | 10.75 | 27.18 | 2 | 12.47 | 27.46 |
| CV (%) | 4% | 4.8% | 6.9% | 5% | 5.4% | 6.9% |
We measured the performance consistency of Mouse MCP-1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 39 | 36 | 34 | 34 | 35 | 2.04 | 5.8% |
| Sample 2 | 224 | 228 | 228 | 219 | 224 | 3.69 | 1.6% |
| Sample 3 | 394 | 375 | 431 | 394 | 398 | 20.3 | 5.1% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 30
| Luo YY,Yang ZQ,Lin XF,Zhao FL,Tu HT,Wang LJ,Wen MY,Xian SX.Knockdown of lncRNA PVT1 attenuated macrophage M1 polarization and relieved sepsis induced myocardial injury via miR-29a/HMGB1 axis.Cytokine.2021 Apr 9:155509.doi:10.1016/j.cyto.2021.155509.Epub ahead of print.PMID:33840587. Species: Mouse EK0568 usage in article: APP:ELISA, SAMPLE:BMDMS, DILUTION:NA |
PubMed ID 33840587 |
| Pingjun Zhu,Kun Wan,Ming Yin,Peng Hu,Yifan Que,Xin Zhou,Lei Zhang,Tianzhi Li,Yingzhen Du,Guogang Xu,Xiangqun Fang,"RIPK3 Induces Cardiomyocyte Necroptosis via Inhibition of AMPK-Parkin-Mitophagy in Cardiac Remodelling after Myocardial Infarction",Oxidative Medicine and Cellular Longevity,vol.2021,Article ID 6635955,11 pages,2021.https://doi.org/10.1155/2021/6635955 Species: Mouse EK0568 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
View Publication |
| Liu Y,Qi Y.Vildagliptin, a CD26/DPP4 inhibitor, ameliorates bleomycin-induced pulmonary fibrosis via regulating the extracellular matrix.Int Immunopharmacol.2020 Oct;87:106774.doi:10.1016/j.intimp.2020.106774.Epub 2020 Jul 27. PMID:32731178. Species: Human,Mouse EK0568 usage in article: APP:ELISA, SAMPLE:PLASMA AND LUNG TISSUE, DILUTION:NA |
PubMed ID 32731178 |
| Liu Y,Feng M,Cai J,Li S,Dai X,Shan G,Wu S.Repurposing bortezomib for choroidal neovascularization treatment via antagonizing VEGF-A and PDGF-D mediated signaling.Exp Eye Res.2021 Jan 18;204:108446.doi:10.1016/j.exer.2021.108446.Epub ahead of print.PMID:33 Species: Mouse EK0568 usage in article: APP:ELISA, SAMPLE:RPE TISSUE, DILUTION:NA |
PubMed ID 33476605 |
| Sharma D,Arora S,Banerjee A,Singh J.Improved insulin sensitivity in obese-diabetic mice via chitosan Nanomicelles mediated silencing of pro-inflammatory Adipocytokines.Nanomedicine.2021 Jan 15:102357.doi:10.1016/j.nano.2020.102357.Epub ahead of print. PMI Species: Mouse EK0568 usage in article: APP:ELISA, SAMPLE:ADIPOSE TISSUE, DILUTION:NA |
PubMed ID 33460779 |
| Chen X,He Y,Zhu Y,Du J,Sun H.linc-AAM Facilitates Gene Expression Contributing to Macrophage Activation and Adaptive Immune Responses.Cell Rep.2021 Jan 5;34(1):108584.doi:10.1016/j.celrep.2020.108584.PMID:33406422. Species: Human,Mouse EK0568 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA |
PubMed ID 33406422 |
| Wu,N,Xu,X‐F,Xin,J‐Q, et al. The effects of nuclear factor‐kappa B in pancreatic stellate cells on inflammation and fibrosis of chronic pancreatitis. J Cell Mol Med.2020; 00: 1– 15. https://doi.org/10.1111/jcmm.16213 Species: Mouse EK0568 usage in article: APP:ELISA, SAMPLE:PSCS, DILUTION:NA |
View Publication |
| Wu X, Lin L, Wu H. Ferulic acid alleviates lipopolysaccharide-induced acute lung injury through inhibiting TLR4/NF-κB signaling pathway. J Biochem Mol Toxicol. 2020 Nov 2:e22664. doi: 10.1002/jbt.22664. Epub ahead of print. PMID: 33140555. Species: Mouse EK0568 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA |
PubMed ID 33140555 |
| 3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation | PubMed ID 29887940 |
| Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction | PubMed ID 27355581 |
| Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway | PubMed ID 26305254 |
| 3-(3-Pyridylmethylidene)-2-indolinone Reduces the Severity of Colonic Injury in a Murine Model of Experimental Colitis | PubMed ID 25874026 |
| Yang S, Zhang J, Wang S, Zhao X, Shi J. Oncotarget. 2017 Aug 24;8(53):91185-91198. doi: 10.18632/oncotarget.20434. eCollection 2017 Oct 31. SOCS2 overexpression alleviates diabetic nephropathy in rats by inhibiting the TLR4/NF-κB pathway | PubMed ID 29207635 |
| Du, J., Chen, X., Wang, C., & Sun, H. (2017). Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha. Journal of Ethnopharmacology. Advance online publication. doi: 1... | PubMed ID 29247698 |
| Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ... | PubMed ID 25686540 |
| Wang Zm, Gao W, Wang H, Zhao D, Nie Zl, Shi Jq, Zhao S, Lu X, Wang Ls, Yang Zj. Cell Physiol Biochem. 2014;33(5):1349-58. Doi: 10.1159/000358702. Epub 2014 May 5. Green Tea Polyphenol Epigallocatechin-3-Gallate Inhibits Tnf-??-Induced Production O... | PubMed ID 24852948 |
| Xu Y, Kong X, Zhou H, Zhang X, Liu J, Yan J, Xie H, Xie Y. Thromb Res. 2014 Aug;134(2):384-92. Doi: 10.1016/J.Thromres.2014.05.017. Epub 2014 May 20. Oxldl/??2Gpi/Anti-??2Gpi Complex Induced Macrophage Differentiation To Foam Cell Involving Tlr4/N... | PubMed ID 24882274 |
| Meneses Gc, Lib??rio Ab, De Daher Ef, Da Silva Gb Jr, Da Costa Mf, Pontes Ma, Martins Am. Bmc Infect Dis. 2014 Aug 20;14:451. Doi: 10.1186/1471-2334-14-451. Urinary Monocyte Chemotactic Protein-1 (Mcp-1) In Leprosy Patients: Increased Risk For Kid... | PubMed ID 25142123 |
| Jiang Jx, Zhang Sj, Xiong Yk, Jia Yl, Sun Yh, Lin Xx, Shen Hj, Xie Qm, Yan Xf. Plos One. 2015 Jun 2;10(6):E0128278. Doi: 10.1371/Journal.Pone.0128278. Ecollection 2015. Eets Attenuate Ox-Ldl-Induced Ltb4 Production And Activity By Inhibiting P38 M... | PubMed ID 26035589 |
| Aizman I, Vinodkumar D, Mcgrogan M, Bates D. Stem Cells Dev. 2015 Jul 15;24(14):1623-34. Doi: 10.1089/Scd.2015.0083. Epub 2015 May 20. Cell Injury-Induced Release Of Fibroblast Growth Factor 2: Relevance To Intracerebral Mesenchymal Stromal Cell T... | PubMed ID 25873141 |
| Tu J, Yang L, Zhou X, Qi K, Wang J, Kouadir M, Xu L, Yin X, Zhao D. J Mol Neurosci. 2014 Jan;52(1):107-16. Doi: 10.1007/S12031-013-0140-3. Epub 2013 Nov 13. Prp106-126 And A?? 1-42 Peptides Induce Bv-2 Microglia Chemotaxis And Proliferation. | PubMed ID 24222374 |
| Tu J, Chen B, Yang L, Qi K, Lu J, Zhao D. J Mol Neurosci. 2015 Jun;56(2):509-18. Doi: 10.1007/S12031-015-0553-2. Epub 2015 Apr 14. Amyloid-?? Activates Microglia And Regulates Protein Expression In A Manner Similar To Prions. | PubMed ID 25869610 |
| Zhang R, Jiang F, Chen Cs, Wang T, Feng J, Tao T, Qin X. Mediators Inflamm. 2015;2015:956082. Doi: 10.1155/2015/956082. Epub 2015 May 31. Serum Levels Of Il-1 ?? , Il-6, Tgf- ?? , And Mmp-9 In Patients Undergoing Carotid Artery Stenting And Regula... | PubMed ID 26113783 |
| Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin Dc, Wang Dw. J Cell Physiol. 2015 Sep;230(9):2108-19. Doi: 10.1002/Jcp.24939. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization And Prevent Lps-Induced Cardiac Dysfunction. | PubMed ID 25626689 |
| Kong J, Kong L, Kong J, Ke S, Gao J, Ding X, Zheng L, Sun H, Sun W. J Transl Med. 2012 Nov 21;10:230. Doi: 10.1186/1479-5876-10-230. After Insufficient Radiofrequency Ablation, Tumor-Associated Endothelial Cells Exhibit Enhanced Angiogenesis And P... | PubMed ID 23171368 |
| Liu H, Yang Lx, Guo Rw, Zhu Gf, Shi Yk, Wang Xm, Qi F, Guo Cm, Ye Js, Yang Zh, Liang X. Int J Cardiol. 2013 Oct 9;168(4):3248-53. Doi: 10.1016/J.Ijcard.2013.04.141. Epub 2013 May 1. Functional Blockage Of Emmprin Ameliorates Atherosclerosis In Apo... | PubMed ID 23642811 |
| Lin Y, Liu Hl, Fang J, Yu Ch, Xiong Yk, Yuan K. Food Chem Toxicol. 2014 Jun;68:290-6. Doi: 10.1016/J.Fct.2014.03.026. Epub 2014 Mar 29. Anti-Fatigue And Vasoprotective Effects Of Quercetin-3-O-Gentiobiose On Oxidative Stress And Vascular Endotheli... | PubMed ID 24685824 |
| Jiang Jx, Zhang Sj, Liu Yn, Lin Xx, Sun Yh, Shen Hj, Yan Xf, Xie Qm. Eur J Pharmacol. 2014 Mar 15;727:43-51. Doi: 10.1016/J.Ejphar.2014.01.045. Epub 2014 Jan 31. Eets Alleviate Ox-Ldl-Induced Inflammation By Inhibiting Lox-1 Receptor Expression In... | PubMed ID 24486707 |
| Huang Wy, Wang J, Liu Ym, Zheng Qs, Li Cy. Eur J Pharmacol. 2014 Jan 15;723:67-72. Doi: 10.1016/J.Ejphar.2013.11.041. Epub 2013 Dec 11. Inhibitory Effect Of Malvidin On Tnf-??-Induced Inflammatory Response In Endothelial Cells. | PubMed ID 24333549 |
| Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce... | PubMed ID 21647942 |
Submit your review to [email protected] or to Biocompare.com for an Amazon giftcard.
1 reviews
"Good reproducible estimation"
Source: Biocompare.com
"Good reproducible estimation"
--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student
| Application | ELISA |
|---|---|
| Sample | Centrifuged cell culture media supernatant |
| Detection | -- |
"Had to perform the analysis twice initially as the background wasn't clear. Overall, good reproducible estimation. Reliable, reproducible. Good product but would appreciate a faster assay kit with less chances of manual error." (Customer’s Tip: "Perform washing steps at least 6-7 times to get a clearer background with 60 seconds wait period in between each washing.")
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0568 Mouse MCP-1 ELISA Kit PicoKine™
7 Related Questions
Question
Q: how do I analyze ELISA data? I have performed CCL2 level in serum.
Verified Customer
Asked: 2020-08-07
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-08-07
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2019-06-01
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-06-01
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
W. Wright
Asked: 2017-08-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-08-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
C. Young
Asked: 2014-08-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2014-08-26
Question
Q: if the enzyme conjugated CCL2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL2 antigen?
Verified Customer
Asked: 2014-05-22
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-05-22
Question
Can the sample diluent buffer, ABC diluent buffer and antibody diluent buffer be used interchangeably between the kit?
Asked: 2019-05-28
Answer
For the Mouse MCP-1 ELISA Kit PicoKine™ (EK0568), the sample diluent buffer, ABC diluent buffer and antibody diluent buffer can be used interchangeably between the kit. However, the sample diluent buffer can only be used to dilute standards and samples, ABC diluent buffer is used to dilute ABC only and antibody diluent buffer is used to dilute antibody only.
Boster Scientific Support
Answered: 2019-05-29
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| (888) 466-3604 |
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