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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).|
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Human EphA1 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human EphA1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human EPHA1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human EphA1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1796)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: K26-E547
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1796 is reactive to EPHA1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
EK1796 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1796-CAP||Anti-Human EPHA1 Pre-coated 96-well strip microplate||1|
|EK1796-ST||Human EPHA1 Standard||2 vials, 10 ng/tube|
|EK1796-DA||Human EPHA1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human EphA1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect EPHA1, Dilution ratio of 1:1, concentration in serum is around 4ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human EphA1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of EPHA1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For EPHA1 (Source: Uniprot.Org, NCBI)
Ephrin type-A receptor 1
protein kinase superfamily
EC 2.7.10; EC 220.127.116.11; EPH receptor A1; eph tyrosine kinase 1; EphA1; EPHephrin type-A receptor 1; EPHT; EPHT1erythropoietin-producing hepatoma amplified sequence; Esk; MGC163163; oncogene EPH; soluble EPHA1 variant 1; soluble EPHA1 variant 2; Tyrosine-protein kinase receptor EPH EPHA1 EPH, EPHT, EPHT1 EPH receptor A1 ephrin type-A receptor 1|eph tyrosine kinase 1|erythropoietin-producing hepatoma amplified sequence|erythropoietin-producing hepatoma receptor|hEpha1|oncogene EPH|soluble EPHA1 variant 1|soluble EPHA1 variant 2|tyrosine-protein kinase receptor EPH*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on EPHA1, check out the EPHA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EPHA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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