Anti-Estrogen Receptor beta/ESR2 Antibody
Rabbit IgG polyclonal antibody for Estrogen receptor beta(ESR2) detection. Tested with WB in Human.
Anti-Estrogen Receptor beta/ESR2 Antibody Info At A Glance
|Product Name||Anti-Estrogen Receptor beta/ESR2 Antibody
See all ER beta/NR3A2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for ESTROGEN RECEPTOR BETA/ESR2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. ESTROGEN RECEPTOR BETA/ESR2 information: Molecular Weight: 59216 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.|
|Cite This Product||Anti-Estrogen Receptor beta/ESR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1126)|
|Similar Products From
|Anti-Estrogen Receptor beta/ESR2 Antibody may replace the following items: sc 79772|sc 79769.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Estrogen Receptor beta(27-50aa HGSIYIPSSYVDSHHEYPAMTFYS), different from the related rat and mouse sequences by four amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For ESR2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||2100|
|Species Of This Entry||Human|
|Protein Name||Estrogen receptor beta|
|Superfamily||nuclear hormone receptor family|
|Alternative Names||ER beta/NR3A2|ER beta; Erb; ER-beta; ESR2; ESR-BETA; ESTRB; estrogen receptor 2 (ER beta); estrogen receptor beta 4; estrogen receptor beta; NR3A2; NR3A2ESRB; Nuclear receptor subfamily 3 group A member 2|
|Post Tranlational Modifications||Phosphorylation; Methylation; Demethylation; Hydroxylation|
|Gene Location||14q23.2-q23.3, on Chromasome 14, gene sequence: NC_000014.9 (64226707..64338631, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ESR2 (Source: Uniprot.org, NCBI)
|Protein Function||Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.|
|Tissue Specificity||Isoform 1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform 2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform 4 is found in testis. Isoform 5 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform 6 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.|
|Interacting Proteins||BRCA1; CCDC62; CCNDBP1; CTGF; EGFR; FOXO3; MDFI; Ncor1; PLSCR1; PPP5C; TFCP2|
|Research Areas||Breast Cancer; Cancer; GPCR|
|Related Diseases||Malignant Neoplasms; Malignant Neoplasm Of Breast; Mammary Neoplasms; Neoplasms; Infertility; Cardiovascular Diseases; Osteoporosis; Endometriosis, Site Unspecified; Dysequilibrium Syndrome; Pituitary Diseases; Malignant Neoplasm Of Prostate; Fracture; Cholangiocarcinoma; Colorectal Cancer; Hypertensive Disease; Carcinogenesis; Osteoporosis, Postmenopausal; Insulin Resistance; Obesity; Endometrial Polyp|
|Related Pathways||Pathogenesis; Cell Proliferation; Localization; Ovulation; Secretion; Spermatogenesis; Aging; Menopause; Methylation; Transport; Menarche; Cell Division; Cotyledon Development; Dna Methylation; Regeneration; Sertoli Cell Proliferation; Cell Growth; Angiogenesis; Sex Determination; Cognition|
|Background||Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.|
|Scientific References||PMID: 9473491 by Ogawa S., et al. The complete primary structure of human estrogen receptor beta (hERbeta) and its heterodimerization with ER alpha in vivo and in vitro.|
PMID: 9636657 by Moore J.T., et al. Cloning and characterization of human estrogen receptor beta isoforms.
Our Boster Quality Guarantee for Anti-Estrogen Receptor beta/ESR2 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
WB: MCF-7 Cell Lysate
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
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- Flow Cytometry Technical Resource Center
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