Anti-ETS1 Monoclonal Antibody

Boster Bio Anti-ETS1 Monoclonal Antibody catalog # A00931-1. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A00931-1
Size: 100μl
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IF, IHC, WB

Product Name

Anti-ETS1 Monoclonal Antibody

See all Ets-1 products

SKU/Catalog Number

A00931-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-ETS1 Monoclonal Antibody catalog # A00931-1. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ETS1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00931-1)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clone Number

8A8

Immunogen

Purified recombinant fragment of human ETS1 expressed in E. Coli.

Reactive Species

A00931-1 is reactive to ETS1 in Human

Applications

A00931-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC, 1:200-1:1000
IF, 1:200-1:1000
FC, 1:200-1:400

Validation Images & Assay Conditions

Gene/Protein Information For ETS1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P14921

Gene ID

2113

Gene Name

ETS1

Full Name

Protein C-ets-1

Weight

50.408kDa

Superfamily

ETS family

Alternative Names

Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1; ets protein; Ets1; Ets-1; EWSR2; FLJ10768; p54; Protein C-Ets-1; v-ets avian erythroblastosis virus E2 oncogene homolog 1; v-ets avian erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); v-ets ETS1 ETS-1, EWSR2, c-ets-1, p54 ETS proto-oncogene 1, transcription factor protein C-ets-1|Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1|v-ets avian erythroblastosis virus E2 oncogene homolog 1|v-ets avian erythroblastosis virus E26 oncogene homolog 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ETS1, check out the ETS1 Infographic

ETS1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ETS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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16 Customer Q&As for Anti-ETS1 Monoclonal Antibody

Question

I am interested in using your anti-ETS1 Monoclonal antibody for negative regulation of inflammatory response studies. Has this antibody been tested with western blotting on jurkat cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

Thank you for your inquiry. This A00931-1 anti-ETS1 Monoclonal antibody is tested on jurkat cells. It is guaranteed to work for Flow Cytometry, IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-27

Question

Is this A00931-1 anti-ETS1 Monoclonal antibody reactive to the isotypes of ETS1?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

The immunogen of A00931-1 anti-ETS1 Monoclonal antibody is Purified recombinant fragment of human ETS1 expressed in E. Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-11

Question

We have seen staining in human endometrium. Do you have any suggestions? Is anti-ETS1 Monoclonal antibody supposed to stain endometrium positively?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

From what I have seen in literature endometrium does express ETS1. From what I have seen in Uniprot.org, ETS1 is expressed in blood, endometrium, lung, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have ETS1 expression, here are a few articles citing expression in various tissues:
Endometrium, Pubmed ID: 17974005
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-01-29

Question

Our lab want to know about to test anti-ETS1 Monoclonal antibody A00931-1 on human erythroleukemia for research purposes, then I may be interested in using anti-ETS1 Monoclonal antibody A00931-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

The products we sell, including anti-ETS1 Monoclonal antibody A00931-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-27

Question

We were content with the WB result of your anti-ETS1 Monoclonal antibody . However we have been able to see positive staining in blood cytoplasm. using this antibody. Is that expected? Could you tell me where is ETS1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-15

Answer

From literature, blood does express ETS1. Generally ETS1 expresses in cytoplasm. Regarding which tissues have ETS1 expression, here are a few articles citing expression in various tissues:
Endometrium, Pubmed ID: 17974005
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-01-15

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-ETS1 Monoclonal antibody A00931-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-15

Question

My question regarding product A00931-1, anti-ETS1 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00931-1 anti-ETS1 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-24

Question

I see that the anti-ETS1 Monoclonal antibody A00931-1 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-05-22

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-05-22

Question

See below the WB image, lot number and protocol we used for erythroleukemia using anti-ETS1 Monoclonal antibody A00931-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-04-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-11

Question

Would anti-ETS1 Monoclonal antibody A00931-1 work for IF with erythroleukemia?

Verified Customer

Verified customer

Asked: 2018-01-18

Answer

According to the expression profile of erythroleukemia, ETS1 is highly expressed in erythroleukemia. So, it is likely that anti-ETS1 Monoclonal antibody A00931-1 will work for IF with erythroleukemia.

Boster Scientific Support

Answered: 2018-01-18

Question

I was wanting to use your anti-ETS1 Monoclonal antibody for IF for human erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2018-01-01

Answer

You can see on the product datasheet, A00931-1 anti-ETS1 Monoclonal antibody has been tested for Flow Cytometry, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-01

Question

Is a blocking peptide available for product anti-ETS1 Monoclonal antibody (A00931-1)?

Verified Customer

Verified customer

Asked: 2017-11-24

Answer

We do provide the blocking peptide for product anti-ETS1 Monoclonal antibody (A00931-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-24

Question

Does A00931-1 anti-ETS1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-05-15

Answer

You can see on the product datasheet, A00931-1 anti-ETS1 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-05-15

Question

Do you have a BSA free version of anti-ETS1 Monoclonal antibody A00931-1 available?

H. Yang

Verified customer

Asked: 2016-08-09

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ETS1 Monoclonal antibody A00931-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-08-09

Question

Our lab used your anti-ETS1 Monoclonal antibody for Flow Cytometry on lung a few months ago. I am using human, and We want to use the antibody for WB next. I was wanting to use examining lung as well as endometrium in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

B. Carter

Verified customer

Asked: 2016-06-21

Answer

I have checked the website and datasheets of our anti-ETS1 Monoclonal antibody and it seems that A00931-1 has been validated on human in both Flow Cytometry and WB. Thus A00931-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2016-06-21

Question

We are currently using anti-ETS1 Monoclonal antibody A00931-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

J. Anderson

Verified customer

Asked: 2013-12-27

Answer

The anti-ETS1 Monoclonal antibody (A00931-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-12-27

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A00931-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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