Product Info Summary
SKU: | M07091 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC |
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Product info
Product Name
Anti-Integrin alpha E ITGAE Rabbit Monoclonal Antibody
SKU/Catalog Number
M07091
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Integrin alpha E ITGAE Rabbit Monoclonal Antibody catalog # M07091. Tested in IHC application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin alpha E ITGAE Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07091)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AbI91
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Integrin alpha E
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M07091 is reactive to ITGAE in Human
Applications
M07091 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
22 kDa
Calculated molecular weight
130.159kDa
Background of ITGAE
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100-1:500
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human tonsil, using Integrin alpha E Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ITGAE (Source: Uniprot.org, NCBI)
Gene Name
ITGAE
Full Name
Integrin alpha-E
Weight
130.159kDa
Superfamily
integrin alpha chain family
Alternative Names
antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103 antigen; CD103; HML-1 antigen; HUMINAE; Integrin alpha E; integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide); ITGAE; MGC141996; Mucosal lymphocyte 1 antigen ITGAE CD103, HUMINAE integrin subunit alpha E integrin alpha-E|HML-1 antigen|antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide|human mucosal lymphocyte antigen 1, alpha polypeptide|integrin alpha-IEL|integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)|mucosal lymphocyte 1 antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGAE, check out the ITGAE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGAE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Integrin alpha E ITGAE Rabbit Monoclonal Antibody (M07091)
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No publications found for M07091
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Integrin alpha E ITGAE Rabbit Monoclonal Antibody
Question
I have a question about product M07091, anti-Integrin alpha E Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M07091 anti-Integrin alpha E Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-21
Question
We are currently using anti-Integrin alpha E Rabbit Monoclonal antibody M07091 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
K. Li
Verified customer
Asked: 2020-03-06
Answer
The anti-Integrin alpha E Rabbit Monoclonal antibody (M07091) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-06
Question
Is this M07091 anti-Integrin alpha E Rabbit Monoclonal antibody reactive to the isotypes of ITGAE?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The immunogen of M07091 anti-Integrin alpha E Rabbit Monoclonal antibody is A synthesized peptide derived from human Integrin alpha E. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-27
Question
Does M07091 anti-Integrin alpha E Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
You can see on the product datasheet, M07091 anti-Integrin alpha E Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-15
Question
I have attached the WB image, lot number and protocol we used for oocyte using anti-Integrin alpha E Rabbit Monoclonal antibody M07091. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-03
Question
Is a blocking peptide available for product anti-Integrin alpha E Rabbit Monoclonal antibody (M07091)?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
We do provide the blocking peptide for product anti-Integrin alpha E Rabbit Monoclonal antibody (M07091). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-10
Question
I was wanting to use your anti-Integrin alpha E Rabbit Monoclonal antibody for IHC for human oocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human oocyte identification?
J. Mangal
Verified customer
Asked: 2013-01-18
Answer
You can see on the product datasheet, M07091 anti-Integrin alpha E Rabbit Monoclonal antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-18