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Product Info Summary
| SKU: | M07091 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Integrin alpha E ITGAE Rabbit Monoclonal Antibody
SKU/Catalog Number
M07091
BM4493 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Integrin alpha E ITGAE Rabbit Monoclonal Antibody catalog # M07091. Tested in IHC application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin alpha E ITGAE Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07091)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AbI91
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Integrin alpha E
Reactive Species
M07091 is reactive to ITGAE in Human
Observed Molecular Weight
130 kDa
Calculated molecular weight
130.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07091 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:50-200
Positive Control
IHC: human cervical cancer, human tonsil tissue
Validation Images & Assay Conditions
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IHC analysis of ITGAE using anti-ITGAE antibody (M07091).
ITGAE was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-ITGAE Antibody (M07091) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of ITGAE using anti-ITGAE antibody (M07091).
ITGAE was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-ITGAE Antibody (M07091) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Integrin alpha E ITGAE Rabbit Monoclonal Antibody (M07091)
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Customer Reviews
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7 Customer Q&As for Anti-Integrin alpha E ITGAE Rabbit Monoclonal Antibody
Question
I have a question about product M07091, anti-Integrin alpha E Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M07091 anti-Integrin alpha E Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-21
Question
We are currently using anti-Integrin alpha E Rabbit Monoclonal antibody M07091 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
K. Li
Verified customer
Asked: 2020-03-06
Answer
The anti-Integrin alpha E Rabbit Monoclonal antibody (M07091) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-06
Question
Is this M07091 anti-Integrin alpha E Rabbit Monoclonal antibody reactive to the isotypes of ITGAE?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The immunogen of M07091 anti-Integrin alpha E Rabbit Monoclonal antibody is A synthesized peptide derived from human Integrin alpha E. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-27
Question
Does M07091 anti-Integrin alpha E Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
You can see on the product datasheet, M07091 anti-Integrin alpha E Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-15
Question
I have attached the WB image, lot number and protocol we used for oocyte using anti-Integrin alpha E Rabbit Monoclonal antibody M07091. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-03
Question
Is a blocking peptide available for product anti-Integrin alpha E Rabbit Monoclonal antibody (M07091)?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
We do provide the blocking peptide for product anti-Integrin alpha E Rabbit Monoclonal antibody (M07091). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-12-10
Question
I was wanting to use your anti-Integrin alpha E Rabbit Monoclonal antibody for IHC for human oocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human oocyte identification?
J. Mangal
Verified customer
Asked: 2013-01-18
Answer
You can see on the product datasheet, M07091 anti-Integrin alpha E Rabbit Monoclonal antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-18
