Human CD56/NCAM-1 ELISA Kit PicoKine™

Human CD56/NCAM-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human NCAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0706
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human CD56/NCAM-1 ELISA Kit PicoKine™

See all NCAM-1/CD56 products

SKU/Catalog Number

EK0706

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD56/NCAM-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human NCAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CD56/NCAM-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0706)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-G718

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0706 is reactive to NCAM1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0706 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0706-CAP Anti-Human NCAM1 Pre-coated 96-well strip microplate 1
EK0706-ST Human NCAM1 Standard 2 vials, 10 ng/tube
EK0706-DA Human NCAM1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0160.1130.2010.3340.6731.1371.5882.042

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect NCAM-1/CD56, Dilution ratio of 1:1, concentration in serum is around 200ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD56/NCAM-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3191085557031311025558
Standard deviation16.2644.48239.5117.2150.69316.8
CV (%)5.7%%4.1%4.3%5.5%4.6%5.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of NCAM-1/CD56 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131934131329531716.435.1%
Sample 21085118910301018108067.546.2%
Sample 355705244597357625637268.074.7%
*number of samples for each test n=16.

Gene/Protein Information For NCAM1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P13591

Gene ID

4684

Gene Name

NCAM1

Full Name

Neural cell adhesion molecule 1

Weight

94.574kDa

Alternative Names

CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM NCAM1 CD56, MSK39, NCAM neural cell adhesion molecule 1 neural cell adhesion molecule 1|antigen recognized by monoclonal antibody 5.1H11|neural cell adhesion molecule, NCAM

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NCAM1, check out the NCAM1 Infographic

NCAM1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NCAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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9 Customer Q&As for Human CD56/NCAM-1 ELISA Kit PicoKine™

Question

Q: Can CD56 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-12-18

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-12-18

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

D. Zhang

Verified customer

Asked: 2020-12-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-12-13

Question

Q: how can I thaw whole blood sample for CD56 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-03-22

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD56 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-03-22

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CD56 using the EK0706 Human CD56 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2019-10-23

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-10-06

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-10-06

Question

Have you received any reports from other customers who have experienced issues using frozen samples with EK0706 kit?

Verified customer

Asked: 2019-05-01

Answer

We have not received any reports from customers having issues with frozen serum or plasma samples using our Human CD56/NCAM-1 ELISA Kit PicoKine™ (EK0706).

Boster Scientific Support

Answered: 2019-05-01

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

L. Turner

Verified customer

Asked: 2018-06-16

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-06-16

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

L. Walker

Verified customer

Asked: 2014-06-06

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2014-06-06

Question

Q: if the enzyme conjugated CD56 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD56 antigen?

M. Dayal

Verified customer

Asked: 2014-05-05

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2014-05-05

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