Product Info Summary
SKU: | A00188 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-FLT3(Ab-591) Antibody
View all Flt-3/Flk-2/CD135 Antibodies
SKU/Catalog Number
A00188
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-FLT3(Ab-591) Antibody (Catalog # A00188). Tested in WB, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FLT3(Ab-591) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00188)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00188 is reactive to FLT3 in Human
Reconstitution
Calculated molecular weight
112903 MW
Background of Flt-3/Flk-2/CD135
FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Sekine S et,al. (2008) J Immunol. ;180(12):8126-34
Pratz K et,al. (2008) Leuk Lymphoma. 2008 ;49(5):852-63.
Al Shaer L et,al. (2008) Br J Haematol. 141(4):483-93.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00188 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 130 160 kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from 293 and MCF cells using FLT3(Ab-591) antibody A00188.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using FLT3(Ab-591) antibody A00188.
Protein Target Info & Infographic
Gene/Protein Information For FLT3 (Source: Uniprot.org, NCBI)
Gene Name
FLT3
Full Name
Receptor-type tyrosine-protein kinase FLT3
Weight
112903 MW
Superfamily
protein kinase superfamily
Alternative Names
Receptor-type tyrosine-protein kinase FLT3;2.7.10.1;FL cytokine receptor;Fetal liver kinase-2;FLK-2;Fms-like tyrosine kinase 3;FLT-3;Stem cell tyrosine kinase 1;STK-1;CD135;FLT3;CD135, FLK2, STK1; FLT3 CD135, FLK-2, FLK2, STK1 fms related receptor tyrosine kinase 3 receptor-type tyrosine-protein kinase FLT3|CD135 |FL cytokine receptor|fetal liver kinase 2|fms related tyrosine kinase 3|fms-like tyrosine kinase 3|growth factor receptor tyrosine kinase type III|stem cell tyrosine kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FLT3, check out the FLT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FLT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FLT3(Ab-591) Antibody (A00188)
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No publications found for A00188
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-FLT3(Ab-591) Antibody
Question
We are currently using anti-FLT3(Ab-591) antibody A00188 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
The anti-FLT3(Ab-591) antibody (A00188) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-20
Question
Is there a BSA free version of anti-FLT3(Ab-591) antibody A00188 available?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FLT3(Ab-591) antibody A00188 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-11
Question
We were satisfied with the WB result of your anti-FLT3(Ab-591) antibody. However we have observed positive staining in testis membrane using this antibody. Is that expected? Could you tell me where is FLT3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
From literature, testis does express FLT3. Generally FLT3 expresses in membrane. Regarding which tissues have FLT3 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7507245
Lymphocyte, Pubmed ID: 8394751, 15057823
Testis, Pubmed ID: 2004790
Boster Scientific Support
Answered: 2019-11-08
Question
See below the WB image, lot number and protocol we used for cerebellar hemisphere using anti-FLT3(Ab-591) antibody A00188. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-06
Question
We ordered your anti-FLT3(Ab-591) antibody for IF on testis in the past. I am using human, and I plan to use the antibody for WB next. We are interested in examining testis as well as cerebellar hemisphere in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
I have checked the website and datasheets of our anti-FLT3(Ab-591) antibody and I see that A00188 has been validated on human in both IF and WB. Thus A00188 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-01
Question
Is this A00188 anti-FLT3(Ab-591) antibody reactive to the isotypes of FLT3?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
The immunogen of A00188 anti-FLT3(Ab-591) antibody is Peptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-25
Question
I see that the anti-FLT3(Ab-591) antibody A00188 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-14
Question
Would A00188 anti-FLT3(Ab-591) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Wu
Verified customer
Asked: 2019-07-17
Answer
As indicated on the product datasheet, A00188 anti-FLT3(Ab-591) antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-17
Question
We have seen staining in human cerebellar hemisphere. Any tips? Is anti-FLT3(Ab-591) antibody supposed to stain cerebellar hemisphere positively?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
From what I have seen in literature cerebellar hemisphere does express FLT3. From what I have seen in Uniprot.org, FLT3 is expressed in cerebellar hemisphere, bone marrow, lymphocyte, testis, among other tissues. Regarding which tissues have FLT3 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7507245
Lymphocyte, Pubmed ID: 8394751, 15057823
Testis, Pubmed ID: 2004790
Boster Scientific Support
Answered: 2019-05-27
Question
I am interested in using your anti-FLT3(Ab-591) antibody for hematopoietic progenitor cell differentiation studies. Has this antibody been tested with western blotting on mcf cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-02-22
Answer
We appreciate your inquiry. This A00188 anti-FLT3(Ab-591) antibody is tested on mcf cells, hela cells. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-22
Question
Is a blocking peptide available for product anti-FLT3(Ab-591) antibody (A00188)?
Verified Customer
Verified customer
Asked: 2018-12-28
Answer
We do provide the blocking peptide for product anti-FLT3(Ab-591) antibody (A00188). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-28
Question
My question regarding product A00188, anti-FLT3(Ab-591) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00188 anti-FLT3(Ab-591) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-01
Question
I was wanting to use your anti-FLT3(Ab-591) antibody for IF for human cerebellar hemisphere on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cerebellar hemisphere identification?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
You can see on the product datasheet, A00188 anti-FLT3(Ab-591) antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cerebellar hemisphere in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-19
Question
Will anti-FLT3(Ab-591) antibody A00188 work for IF with cerebellar hemisphere?
S. Miller
Verified customer
Asked: 2017-01-26
Answer
According to the expression profile of cerebellar hemisphere, FLT3 is highly expressed in cerebellar hemisphere. So, it is likely that anti-FLT3(Ab-591) antibody A00188 will work for IF with cerebellar hemisphere.
Boster Scientific Support
Answered: 2017-01-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellar hemisphere using anti-FLT3(Ab-591) antibody A00188. Let me know if you need anything else.
A. Kulkarni
Verified customer
Asked: 2015-02-27
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-27
Question
We want to test anti-FLT3(Ab-591) antibody A00188 on human cerebellar hemisphere for research purposes, then I may be interested in using anti-FLT3(Ab-591) antibody A00188 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Wu
Verified customer
Asked: 2014-12-30
Answer
The products we sell, including anti-FLT3(Ab-591) antibody A00188, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-12-30