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- Table of Contents
FOXO3 antibody (M00252) performs well in IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Immunofluorescent analysis of Hela cells, using FoxO3a Antibody.
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Anti-FoxO3a Rabbit Monoclonal Antibody
FOXO3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, ICC, WB |
Tested Applications
FOXO3 antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-FoxO3a Rabbit Monoclonal Antibody
View all FOXO3 Antibodies
View all FOXO3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HFD-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FoxO3a
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
90 kDa
Calculated Molecular Weight
71.3 kDa
Cite This Product
Anti-FoxO3a Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00252)
Protocols
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FOXO3 antibody
Publications
Application:
Species:
(2E)-1-(2,4,6-Trimethoxyphenyl)-3-(4-chlorophenyl)prop-2-en-1-one, a Chalcone Derivative, Promotes Apoptosis by Suppressing RAS-ERK and AKT/FOXO3a Pathways in Hepatocellular Carcinoma Cells
Published: 2023-06-14
Journal: CHEMISTRY & BIODIVERSITY
Application: WB
Impact Factor: 2.90
Species: Human
PMID: 37314937
Calycosin alleviates cerebral ischemia/reperfusion injury by repressing autophagy via STAT3/FOXO3a signaling pathway
Published: 2023-04-28
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 7.90
Species: Rat
PMID: 37148714
Ribosomal protein RPL5 regulates colon cancer cell proliferation and migration through MAPK/ERK signaling pathway
Published: 2022-11-16
Journal: BMC Molecular and Cell Biology
Application: WB
Impact Factor: 2.81
Species: Human
PMID: 36384455
Holliday junction‑recognition protein modulates apoptosis, cell cycle arrest and reactive oxygen species stress in human renal cell carcinoma
Published: 2020-06-22
Journal: ONCOLOGY REPORTS
Application: WB
Impact Factor: 3.42
Species: Human
PMID: 32582972
Review
Have you used Anti-FoxO3a Rabbit Monoclonal Antibody?
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1 Review For Anti-FoxO3a Rabbit Monoclonal Antibody
The Anti-FOXO3A antibody (M00252) produced clear, specific bands with low background, and reliably detected expected changes in FOXO3A levels in normal, Alzheimer’s model, and treated mouse hippocampal tissues, demonstrating excellent performance.
| SKU | M00252 |
|---|---|
| Application | Western Blot |
| Sample | mouse brain tissue |
| Sample Processing Description | ① Normal mouse hippocampal tissue, ② Hippocampal tissue from Alzheimer’s disease model mouse, ③ Hippocampal tissue from Alzheimer’s disease model mouse treated with a self-developed drug. Total protein was extracted from all samples. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | TNF alpha Antibody Picoband® |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) |
| Secondary Incubation | 1:10000, 1 h in RT |
| Detection | Substrate: ECL substrate, Image system: ChemiDoc MP |
| Results Summary | FOXO3a is a key member of the FOXO transcription factor family, playing a central regulatory role in cellular processes such as stress response, apoptosis, autophagy, metabolism, and antioxidation. In Alzheimer’s disease, FOXO3a is known to exert neuroprotective effects. Experimental results show that FOXO3a protein levels are significantly decreased in the brains of Alzheimer’s model mice, while treatment leads to a noticeable increase. |
Huili Yin, Shandong First Medical University
Verified customer
Submitted 2026-03-30
Product Promise
We promise Anti-FoxO3a Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00252
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026