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Product Info Summary
| SKU: | A00252 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Forkhead box protein O3 FOXO3 Antibody
SKU/Catalog Number
A00252
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Forkhead box protein O3 FOXO3 Antibody (Catalog # A00252). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
FOXO3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Forkhead box protein O3 FOXO3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00252)
Host
Rabbit
Contents
FOXO3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FOXO3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human FOXO3. The immunogen is located within amino acids 40 - 90 of FOXO3.
Reactive Species
A00252 is reactive to FOXO3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
71.3 kDa
Background of FOXO3
FOXO3 is a ubiquitously expressed 75 kDa protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. FOXO3 is involved with mTOR in the regulation of autophagy in skeletal muscle, and activates protein degradation in atrophying muscle cells. FOXO3 has also been implicated in several neurodegenerative disorders including aging, neuromuscular disease, systemic lupus erythmatosus, stroke and diabetic complications.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00252 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FOXO3 antibody can be used for detection of FOXO3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FOXO3 in A-20 cell lysate with FOXO3 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry of FOXO3 in A-20 cells with FOXO3 antibody at 4 μg/mL.
Specific Publications For Anti-Forkhead box protein O3 FOXO3 Antibody (A00252)
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