Data & Images
|Product Name||Anti-Fractalkine/CX3CL1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Fractalkine(CX3CL1) detection. Tested with WB in Human.|
|Cite This Product||Anti-Fractalkine/CX3CL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1400)|
|Replacement Item||This antibody may replace the following items: sc-137046|sc-166200|sc-20730|sc-20973|sc-21382|sc-21384|sc-7225|sc-7226 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human CX3CL1(50-64aa HYQQNQASCGKRAII).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||42203 MW|
|Protein Function||The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. .|
|Tissue Specificity||Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.|
|Sequence Similarities||Belongs to the intercrine delta family.|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein.|
|Alternative Names||Fractalkine;C-X3-C motif chemokine 1;CX3C membrane-anchored chemokine;Neurotactin;Small-inducible cytokine D1;Processed fractalkine;CX3CL1;FKN, NTT, SCYD1;A-152E5.2;|
|Research Areas|||immunology|innate immunity|macrophage / inflamm.| immunology|chemokines|delta chemokines (cxc3)| cardiovascular|atherosclerosis|vascular inflammation|leukocyte recruitment||
Background for Fractalkine
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Fractalkine/CX3CL1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Fractalkine/CX3CL1 Antibody Images
Click the images to enlarge.
Lane 1: Recombinant Human Fractalkine Protein 10ng
Lane 2: Recombinant Human Fractalkine Protein 5ng
Lane 3: Recombinant Human Fractalkine Protein 2.5ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,