Anti-CX3CL1 Antibody

Rabbit Polyclonal CX3CL1 Antibody. Validated in WB and tested in Human.

SKU PA1400
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-CX3CL1 Antibody
See all CX3CL1 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1400
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit Polyclonal CX3CL1 Antibody. Validated in WB and tested in Human. Size: 100μg/vial.
Cite This Product Anti-CX3CL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1400)
Specificity Anti-CX3CL1 Antibody (PA1400) reacts with Human CX3CL1, in native form and recombinant. Superfamily members of CX3CL1 are not reactive to PA1400.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-CX3CL1 Antibody (PA1400) was raised against A synthetic peptide corresponding to a sequence at the N-terminus of human CX3CL1(50-64aa HYQQNQASCGKRAII)..
Reactivity Human

Assay Details

Our Boster Quality Guarantee for Anti-CX3CL1 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-CX3CL1 Antibody (PA1400).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/pa1400 1 WB anti fractalkine cx3cl1 antibody.jpg

Anti-CX3CL1 antibody, PA1400, Western blotting
Lane 1: Recombinant Human Fractalkine Protein 10ng
Lane 2: Recombinant Human Fractalkine Protein 5ng
Lane 3: Recombinant Human Fractalkine Protein 2.5ng

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P78423
Gene Name CX3CL1
Protein Name Fractalkine
Tissue Specificity Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.
Alternative Names Fractalkine;C-X3-C motif chemokine 1;CX3C membrane-anchored chemokine;Neurotactin;Small-inducible cytokine D1;Processed fractalkine;CX3CL1;FKN, NTT, SCYD1;A-152E5.2;Show more
Subcellular Localization Cell membrane; Single-pass type I membrane protein.
Molecular Weight 42203 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. .
Research Areas Atherosclerosis, Cardiovascular, Chemokines, Immunology, Innate Immunity, Macrophage / Inflamm., Vascular Inflammation

*You can search these to find other products in these research areas.
Background Chemokine(C-X3-C motif) ligand 1, also known as CX3CL1, is a large cytokine protein. It is said to be the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine(in humans) and neurotactin(in mice). Its gene is mapped to the long arm of chromosome 8. Being the large protein, it has got 373 amino acids. Imai et al.(1997) identified a 7-transmembrane high-affinity receptor(CX3CR1; 601470) for fractalkine and showed that it mediates both the adhesive and migratory functions of fractalkine.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-CX3CL1 Antibody (PA1400)? Please let us know so we can cite the reference in this product datasheet. Email us at support@bosterbio.com.

Order Product (PA1400)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1054 $240
100ug+Free Biotin Secondary BA1003 $240

USD $240

Ships next business day.

Troubleshooting

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Publications

Distribution change of mast cells in human nasal polyps
Distribution change of mast cells in human nasal polyps

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cx3cl1 antibody, fractalkine antibody