Anti-MCP1/CCL2 Antibody Picoband™

Boster Bio Anti-MCP1/CCL2 Antibody Picoband™ catalog # PB9570. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human. Cited in 25 publication(s).

Product Info Summary

SKU: PB9570
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, IHC-P, WB

Product Name

Anti-MCP1/CCL2 Antibody Picoband™

See all CCL2 products

SKU/Catalog Number

PB9570

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MCP1/CCL2 Antibody Picoband™ catalog # PB9570. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MCP1/CCL2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9570)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human MCP-1 recombinant protein (Position: Q24-T99). Human MCP-1 shares 60.9% and 59.4% amino acid (aa) sequence identity with mouse and rat MCP-1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9570 is reactive to CCL2 in Human

Applications

PB9570 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -

Validation Images & Assay Conditions

Gene/Protein Information For CCL2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P13500

Gene ID

6347

Gene Name

CCL2

Full Name

C-C motif chemokine 2

Weight

11.025kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF CCL2 GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF C-C motif chemokine ligand 2 C-C motif chemokine 2|chemokine (C-C motif) ligand 2|monocyte chemoattractant protein-1|monocyte chemotactic and activating factor|monocyte chemotactic protein 1|monocyte secretory protein JE|small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)|small inducible cytokine subfamily A (Cys-Cys), member 2|small-inducible cytokine A2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCL2, check out the CCL2 Infographic

CCL2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9570 has been cited in 25 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of chemokines CCL5 and CCL11 by smooth muscle tumor cells of the uterus and its possible role in the recruitment of mast cells

Salusin-%u03B2 not salusin-%u03B1 promotes vascular inflammation in ApoE-deficient mice via the I-%u03BAB%u03B1/NF-%u03BAB pathway

Comparison of biomarkers in rat renal ischemia-reperfusion injury

Salusin-?, but Not Salusin-?, Promotes Human Umbilical Vein Endothelial Cell Inflammation via the p38 MAPK/JNK-NF-?B Pathway

Bindarit reduces the incidence of acute aortic dissection complicated lung injury via modulating NF-?B pathway

Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm

Effects of vitamin D on renal fibrosis in diabetic nephropathy model rats

Dynamic expressions of monocyte chemo attractant protein-1 and CC chamomile receptor 2 after balloon injury and their effects in intimal proliferation

Attenuation of atherosclerotic lesions in diabetic apolipoprotein E-deficient mice using gene silencing of macrophage migration inhibitory factor

Fluoxetine inhibited extracellular matrix of pulmonary artery and inflammation of lungs in monocrotaline-treated rats

Salusin-?, but Not Salusin-?, Promotes Human Umbilical Vein Endothelial Cell Inflammation via the p38 MAPK/JNK-NF-?B Pathway

IL-1? mediates MCP-1 induction by Wnt5a in gastric cancer cells

Sun C, Fan S, Wang X, Lu J, Zhang Z, Wu D, Shan Q, Zheng Y. J Nutr Biochem. 2015 Oct;26(10):1029-40. Doi: 10.1016/J.Jnutbio.2015.04.012. Epub 2015 May 15. Purple Sweet Potato Color Inhibits Endothelial Premature Senescence By Blocking The Nlrp3 In...

Feng Jx, Hou Ff, Liang M, Wang Gb, Zhang X, Li Hy, Xie D, Tian Jw, Liu Zq. Kidney Int. 2007 May;71(9):901-11. Restricted Intake Of Dietary Advanced Glycation End Products Retards Renal Progression In The Remnant Kidney Model.

He Lj, Liang M, Hou Ff, Guo Zj, Xie D, Zhang X. J Endocrinol. 2009 Mar;200(3):347-55. Doi: 10.1677/Joe-08-0481. Epub 2008 Dec 9. Ethanol Extraction Of Picrorhiza Scrophulariiflora Prevents Renal Injury In Experimental Diabetes Via Anti-Inflammatio...

Tian Y, Lv G, Yang Y, Zhang Y, Yu R, Zhu J, Xiao L, Zhu J. Int J Clin Exp Pathol. 2014 May 15;7(6):3028-37. Ecollection 2014. Effects Of Vitamin D On Renal Fibrosis In Diabetic Nephropathy Model Rats.

Zhang L, Li Hy, Li H, Zhao J, Su L, Zhang Y, Zhang Sl, Miao Jy. J Cell Physiol. 2011 Jun;226(6):1694-701. Doi: 10.1002/Jcp.22500. Lipopolysaccharide Activated Phosphatidylcholine-Specific Phospholipase C And Induced Il-8 And Mcp-1 Production In Va...

Li Hy, Hou Ff, Zhang X, Chen Py, Liu Sx, Feng Jx, Liu Zq, Shan Yx, Wang Gb, Zhou Zm, Tian Jw, Xie D. J Am Soc Nephrol. 2007 Feb;18(2):528-38. Epub 2007 Jan 3. Advanced Oxidation Protein Products Accelerate Renal Fibrosis In A Remnant Kidney Model.

Liu Z, Wang T, Yang J, Wang S, Yang W, Liu J, Ye Z. Mol Med Rep. 2012 May;5(5):1241-4. Doi: 10.3892/Mmr.2012.813. Epub 2012 Feb 28. Calcium Oxalate Monohydrate Crystals Stimulate Monocyte Chemoattractant Protein-1 And Transforming Grow...

Sun D, Bu L, Liu C, Yin Z, Zhou X, Li X, Xiao A. Plos One. 2013 May 28;8(5):E65042. Doi: 10.1371/Journal.Pone.0065042. Print 2013. Therapeutic Effects Of Human Amniotic Fluid-Derived Stem Cells On Renal Interstitial Fibrosis In A Murine Model Of U...

Zhang Z, Wang G, Ma J, Liu H, Zhang X, Zhu G. Exp Ther Med. 2013 Aug;6(2):427-434. Epub 2013 Jun 6. Effect Of Herba Centellae On The Expression Of Hgf And Mcp-1.

Ji X, Gao J, Cai X, Lu W, Hu C, Wang Z, Cheng X, Gu Z, Wan G, Zhang S, Cao P. J Ethnopharmacol. 2011 Apr 12;134(3):624-9. Doi: 10.1016/J.Jep.2011.01.003. Epub 2011 Jan 8. Immunological Regulation Of Chinese Herb Guizhi Fuling Capsule On Rat Endome...

Liu P, Li F, Qiu M, He L. Diabetes Res Clin Pract. 2014 Aug;105(2):206-16. Doi: 10.1016/J.Diabres.2014.04.020. Epub 2014 Apr 28. Expression And Cellular Distribution Of Tlr4, Myd88, And Nf-??b In Diabetic Renal Tubulointerstitial Fibrosis, In Vitr...

Lu Y, Zhu X, Liang Gx, Cui Rr, Liu Y, Wu Ss, Liang Qh, Liu Gy, Jiang Y, Liao Xb, Xie H, Zhou Hd, Wu Xp, Yuan Lq, Liao Ey. Amino Acids. 2012 Nov;43(5):2125-36. Doi: 10.1007/S00726-012-1298-7. Epub 2012 Apr 25. Apelin-Apj Induces Icam-1, Vcam-1 And ...

Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...

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6 Customer Q&As for Anti-MCP1/CCL2 Antibody Picoband™

Question

I purchased your product PB9570 and wondering how many MW (kDa) does this antibody show in Western blot using MCP-1 positive cell lysate?

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

The antibody PB9570 was raised against human MCP-1 thus, the band you will be able to see at 8.6 kDa on Western Blot.

Boster Scientific Support

Answered: 2019-12-12

Question

We have observed staining in human pancreas. Do you have any suggestions? Is anti-MCP1/CCL2 antibody supposed to stain pancreas positively?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

Based on literature pancreas does express CCL2. Based on Uniprot.org, CCL2 is expressed in smooth muscle tissue, glial tumor, urinary bladder, pancreas, among other tissues. Regarding which tissues have CCL2 expression, here are a few articles citing expression in various tissues:
Glial tumor, Pubmed ID: 2465924
Pancreas, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-10-21

Question

We purchased anti-MCP1/CCL2 antibody for IHC-P on glial tumor in the past. I am using human, and I plan to use the antibody for WB next. My question regards examining glial tumor as well as smooth muscle tissue in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

I took a look at the website and datasheets of our anti-MCP1/CCL2 antibody and it appears that PB9570 has been validated on human in both IHC-P and WB. Thus PB9570 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-14

Question

My boss were content with the WB result of your anti-MCP1/CCL2 antibody. However we have seen positive staining in smooth muscle tissue secreted using this antibody. Is that expected? Could you tell me where is CCL2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-01

Answer

According to literature, smooth muscle tissue does express CCL2. Generally CCL2 expresses in secreted. Regarding which tissues have CCL2 expression, here are a few articles citing expression in various tissues:
Glial tumor, Pubmed ID: 2465924
Pancreas, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-05-01

Question

My lab would like using your anti-MCP1/CCL2 antibody for lymphocyte chemotaxis studies. Has this antibody been tested with western blotting on sw620 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-12-07

Answer

Thanks for your inquiry. This PB9570 anti-MCP1/CCL2 antibody is tested on sw620 whole cell lysate. It is guaranteed to work for ELISA, IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-12-07

Question

We are currently using anti-MCP1/CCL2 antibody PB9570 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?

V. Lewis

Verified customer

Asked: 2014-10-31

Answer

The anti-MCP1/CCL2 antibody (PB9570) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-10-31

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