CCL2 antibody (PB9570) performs well in ELISA, IHC, WB and shows reactivity with Human.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 16 Publications
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Anti-MCP1/CCL2 Antibody Picoband®

CCL2 antibody

Reactive SpeciesHostApplication
HumanRabbitELISA, IHC, WB

SKU: PB9570

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

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Quantity: 1

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Tested Applications

CCL2 antibody was positively detected by WB in:

SW620 Whole Cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
ELISA
0.1-0.5μg/ml
-

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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IHC
ELISA
Human
Recommend
Recommend
Monkey
Predicted
Predicted

Product Information

Product Name
Anti-MCP1/CCL2 Antibody Picoband®
View all CCL2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human MCP-1 recombinant protein (Position: Q24-T99). Human MCP-1 shares 60.9% and 59.4% amino acid (aa) sequence identity with mouse and rat MCP-1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated Molecular Weight

11.0 kDa

Background of CCL2
Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.
Cite This Product
Anti-MCP1/CCL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9570)

Protocols

Western Blot protocol for CCL2 antibody

Publications

Application:
Species:
Microglia increase CEMIP expression and promote brain metastasis in breast cancer through the JAK2/STAT3 signaling pathway
Published: 2023-11-21
Journal: Oncologie
Application: IHC
Impact Factor: 0.90
Species: Human
DNA from macrophages induces fibrosis and vasculopathy through POLR3A/STING/type I interferon axis in systemic sclerosis
Published: 2022-06-10
Journal: RHEUMATOLOGY
Application: WB
Impact Factor: 7.05
Species: Human
PMID: 35686918
Tumor-associated macrophages secrete CC-chemokine ligand 2 and induce tamoxifen resistance by activating PI3K/Akt/mTOR in breast cancer
Published: 2019-11-11
Journal: CANCER SCIENCE
Application: ELISA,IHC
Impact Factor: 4.75
Species: Human
PMID: 31710162
Immune imbalance is associated with the development of preeclampsia
Published: 2019-04-05
Journal: MEDICINE
Application: IHC
Impact Factor: 1.87
Species: Human
PMID: 30946359
Expression and Significance of RANTES and MCP-1 in Renal Tissue With Chronic Renal Allograft Dysfunction
Published: 2016-08-26
Journal: TRANSPLANTATION PROCEEDINGS
Application: IHC
Impact Factor: 0.87
Species: Human
PMID: 27569940
Nano-porous nitrocellulose liquid bandage modulates cell and cytokine response and accelerates cutaneous wound healing in a mouse model
Published: 2015-09-25
Journal: CARBOHYDRATE POLYMERS
Application: IHC
Impact Factor: 4.07
Species: Mouse
PMID: 26572394
Lipid metabolism and inflammation modulated by Vitamin D in liver of diabetic rats
Published: 2015-04-18
Journal: Lipids in Health and Disease
Application: IHC
Impact Factor: 2.22
Species: Rat
PMID: 25899686
Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells
Published: 2015-02-19
Journal: CARBOHYDRATE POLYMERS
Application: WB
Impact Factor: 4.07
Species: Human
PMID: 25857968
Valsartan attenuated oxidative stress, decreased MCP-1 and TGF-β1 expression in glomerular mesangial and epithelial cells induced by high-glucose levels
Published: 2015-02-15
Journal: BioScience Trends
Application: ICC
Impact Factor: 1.66
Species: Rat
PMID: 21914953
Salusin-β, but Not Salusin-α, Promotes Human Umbilical Vein Endothelial Cell Inflammation via the p38 MAPK/JNK-NF-κB Pathway
Published: 2014-09-11
Journal: PLoS One
Application: WB
Impact Factor: 3.53
Species: Human
PMID: 25210730
Effects of vitamin D on renal fibrosis in diabetic nephropathy model rats
Published: 2014-05-15
Journal: International Journal of Clinical and Experimental Pathology
Application: IHC
Impact Factor: 1.78
Species: Rat
PMID: 25031721
Expression and cellular distribution of TLR4, MyD88, and NF-κB in diabetic renal tubulointerstitial fibrosis, in vitro and in vivo
Published: 2014-04-28
Journal: DIABETES RESEARCH AND CLINICAL PRACTICE
Application: IHC
Impact Factor: 2.54
Species: Rat
PMID: 24894085
Salusin-β Not Salusin-α Promotes Vascular Inflammation in ApoE-Deficient Mice via the I-κBα/NF-κB Pathway
Published: 2014-03-12
Journal: PLoS One
Application: IHC
Impact Factor: 3.53
Species: Mouse
PMID: 24621517
Ligustilide attenuates inflammatory pain via inhibition of NFκB-mediated chemokines production in spinal astrocytes
Published: 2014-02-12
Journal: EUROPEAN JOURNAL OF NEUROSCIENCE
Application: IHC
Impact Factor: 3.67
Species: Mouse
PMID: 24521480
The Anti-Lung Cancer Activities of Steroidal Saponins of P. polyphylla Smith var. chinensis (Franch.) Hara through Enhanced Immunostimulation in Experimental Lewis Tumor-Bearing C57BL/6 Mice and Induction of Apoptosis in the A549 Cell Line
Published: 2013-10-17
Journal: MOLECULES
Application: WB
Impact Factor: 2.43
Species: Human
PMID: 24141243
Triptolide inhibits CC chemokines expressed in rat adjuvant-induced arthritis
Published: 2006-08-31
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: IHC
Impact Factor: 2.01
Species: Rat
PMID: 17052673

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Product Promise

We promise Anti-MCP1/CCL2 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more

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PB9570
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 13, 2026