Anti-GAD65/GAD2 Antibody Picoband™ (monoclonal, 7G2)

GAD2/GAD65 antibody

Boster Bio Anti-GAD65/GAD2 Antibody Picoband™ (monoclonal, 7G2) catalog # M03142. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M03142
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-GAD65/GAD2 Antibody Picoband™ (monoclonal, 7G2)

View all GAD2/GAD65 Antibodies

SKU/Catalog Number

M03142

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GAD65/GAD2 Antibody Picoband™ (monoclonal, 7G2) catalog # M03142. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAD65/GAD2 Antibody Picoband™ (monoclonal, 7G2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03142)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Monoclonal

Clone Number

7G2

Isotype

Mouse IgG2a

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M03142 is reactive to GAD2 in Human, Mouse, Rat

Applications

M03142 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

62 kDa

Calculated molecular weight

65.411kDa

Background of GAD2/GAD65

Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 4μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For GAD2 (Source: Uniprot.org, NCBI)

Gene Name

GAD2

Full Name

Glutamate decarboxylase 2

Weight

65.411kDa

Superfamily

group II decarboxylase family

Alternative Names

EC 4.1.1; EC 4.1.1.15; GAD2; GAD65; GAD-65; GAD65MGC161605; glutamate decarboxylase 2 (pancreatic islets and brain, 65kD); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase-2 (pancreas); MGC161607,65 kDa glutamic acid decarboxylase GAD2 GAD65 glutamate decarboxylase 2 glutamate decarboxylase 2|65 kDa glutamic acid decarboxylase|GAD-65|Glutamate decarboxylase-2 (pancreas)|glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GAD2, check out the GAD2 Infographic

GAD2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M03142 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Gut microbe-derived metabolite trimethylamine N-oxide induces cardiac hypertrophy and fibrosis Zehua Li, et al. Lab Invest. 2019 Mar;99(3):346-357. doi: 10.1038/s41374-018-0091-y. Epub 2018 Aug 1.

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Order DetailsPrice
M03142

100μg

$370
M03142-10ug

10μg sample (liquid)

$99
M03142-Biotin

100 μg Biotin conjugated

$570
M03142-Cy3

100 μg Cy3 conjugated

$570
M03142-Dylight488

100 μg Dylight488 conjugated

$570
M03142-Dylight550

100 μg Dylight550 conjugated

$570
M03142-Dylight594

100 μg Dylight594 conjugated

$570
M03142-FITC

100 μg FITC conjugated

$570
M03142-HRP

100 μg HRP conjugated

$570
M03142-APC

100 μg APC conjugated

$670
M03142-PE

100 μg PE conjugated

$670
M03142-carrier-free

Carrier Free

$370

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M03142
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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