Click image to see more details
Product Info Summary
| SKU: | M00141 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-RIP RIPK1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00141
BM4850 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RIP RIPK1 Rabbit Monoclonal Antibody catalog # M00141. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RIP RIPK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00141)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IGF-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RIP
Reactive Species
M00141 is reactive to RIPK1 in Human
Observed Molecular Weight
76 kDa
Calculated molecular weight
75.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00141 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
FC 1:20
Positive Control
WB: human Hela whole cell, human K562 whole cell, human Raji whole cell, human Jurkat whole cell
Validation Images & Assay Conditions
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Western blot analysis of RIPK1 using anti-RIPK1 antibody (M00141).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIPK1 antigen affinity purified monoclonal antibody (Catalog # M00141) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIPK1 at approximately 76 kDa. The expected band size for RIPK1 is at 76 kDa.
Specific Publications For Anti-RIP RIPK1 Rabbit Monoclonal Antibody (M00141)
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Customer Q&As
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5 Customer Q&As for Anti-RIP RIPK1 Rabbit Monoclonal Antibody
Question
Could the product recognize c-terminus sequence of RIP or could you provide the sequence of the immunogen?
Verified customer
Asked: 2021-12-12
Answer
The immunogen sequence for M00141 is aa289-490, Uniprot ID:Q13546.
Boster Scientific Support
Answered: 2022-01-14
Question
We have been able to see staining in human cervix carcinoma. Do you have any suggestions? Is anti-RIP Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
According to literature cervix carcinoma does express RIPK1. According to Uniprot.org, RIPK1 is expressed in right lobe of liver, umbilical vein endothelial cell, adrenal gland uterus, brain, leukemic t-cell, cervix carcinoma, erythroleukemia, liver, among other tissues. Regarding which tissues have RIPK1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Uterus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 7538908, 15144186
Liver, Pubmed ID: 24275569
Umbilical vein endothelial cell, Pubmed ID: 8612133
Boster Scientific Support
Answered: 2020-04-17
Question
My colleagues were satisfied with the WB result of your anti-RIP Rabbit Monoclonal antibody. However we have seen positive staining in erythroleukemia cytoplasm. cell membrane. using this antibody. Is that expected? Could you tell me where is RIPK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
From literature, erythroleukemia does express RIPK1. Generally RIPK1 expresses in cytoplasm. cell membrane. Regarding which tissues have RIPK1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Uterus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 7538908, 15144186
Liver, Pubmed ID: 24275569
Umbilical vein endothelial cell, Pubmed ID: 8612133
Boster Scientific Support
Answered: 2019-12-17
Question
We are currently using anti-RIP Rabbit Monoclonal antibody M00141 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
M. Anderson
Verified customer
Asked: 2016-07-12
Answer
The anti-RIP Rabbit Monoclonal antibody (M00141) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-12
Question
I am interested in using your anti-RIP Rabbit Monoclonal antibody for extrinsic apoptotic signaling pathway studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
D. Evans
Verified customer
Asked: 2013-09-24
Answer
Thanks for your inquiry. This M00141 anti-RIP Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-09-24
