Product Info Summary
| SKU: | M08143 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Galectin 13 LGALS13 Antibody (Monoclonal, ABM4E42)
SKU/Catalog Number
M08143
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-Galectin 13 LGALS13 Antibody (Monoclonal, ABM4E42) (Catalog# M08143). Tested in IHC, WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-Galectin 13 LGALS13 Antibody (Monoclonal, ABM4E42) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08143)
Host
Mouse
Contents
100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABM4E42
Isotype
IgG2b Kappa
Immunogen
A full length human Galectin-13 protein.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M08143 is reactive to LGALS13 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
16.1 kDa
Background of LGALS13
Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, This monoclonal antibody reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08143 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
IHC: 5 µg/ml
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of Galectin 13 in human placenta tissue using Galectin 13 antibody (Clone: ABM4E42) at 5 &mu
Click image to see more details
Western blot analysis of Galectin 13. Anti-Galectin 13 antibody (Clone: ABM4E42) was tested at 0.5 &mu
Specific Publications For Anti-Galectin 13 LGALS13 Antibody (Monoclonal, ABM4E42) (M08143)
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