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Product Info Summary
| SKU: | A00922 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IGFBP1 Antibody Picoband®
SKU/Catalog Number
A00922
Size
100 μg/vial
Form
Lyophilized
Description
IGFBP1 regulates IGF biology by binding IGF1/IGF2 and modulating their availability to IGF receptors (IGF signaling axis; endocrine/metabolic and growth contexts). Assay context: tissue localization by IHC and protein-level confirmation by Western blotting are commonly combined to separate compartmental expression vs. total abundance. Often evaluated alongside other IGF-binding proteins such as IGFBP3 and Human IGFBP7 ELISA, or upstream ligand context such as Mouse IGF-2 ELISA (species-appropriate).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGFBP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00922)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IGFBP1 recombinant protein (Position: A69-N259). Human IGFBP1 shares 67.8% and 69.3% amino acid (aa) sequence identity with mouse and rat IGFBP1, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00922 is reactive to IGFBP1 in Human
Observed Molecular Weight
30 kDa
Calculated molecular weight
27.9 kDa
Background of IGFBP1
IGFBP1, Insulin-like growth factor-binding protein 1, also known as placental protein 12 (PP12), is a protein that in humans is encoded by the IGFBP1 gene. The IGFBP1 gene has 4 exons and spans 5.9 kb. And the IGFBP1 gene is localized to 7p13-p12 by in situ hybridization. This gene is a member of the Insulin-like growth factor-binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a type-I thyroglobulin domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00922 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
WB: human HepG2 whole cell, human A549 whole cell
IHC: human placenta tissue
Validation Images & Assay Conditions
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Western blot analysis of IGFBP1 using anti-IGFBP1 antibody (A00922).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP1 antigen affinity purified polyclonal antibody (Catalog # A00922) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP1 at approximately 30 kDa. The expected band size for IGFBP1 is at 28 kDa.
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IHC analysis of IGFBP1 using anti-IGFBP1 antibody (A00922).
IGFBP1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGFBP1 Antibody (A00922) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-IGFBP1 Antibody Picoband® (A00922)
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3 Customer Q&As for Anti-IGFBP1 Antibody Picoband®
Question
Do you have a BSA free version of anti-IGFBP1 antibody A00922 available?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IGFBP1 antibody A00922 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-24
Question
Does anti-IGFBP1 antibody A00922 work on primate WB with decidua?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
Our lab technicians have not validated anti-IGFBP1 antibody A00922 on primate. You can run a BLAST between primate and the immunogen sequence of anti-IGFBP1 antibody A00922 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate decidua in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-29
Question
Is this A00922 anti-IGFBP1 antibody reactive to the isotypes of IGFBP1?
R. Rodriguez
Verified customer
Asked: 2019-06-14
Answer
The immunogen of A00922 anti-IGFBP1 antibody is E.coli-derived human IGFBP1 recombinant protein (Position: A69-N259). Human IGFBP1 shares 67.8% and 69.3% amino acid (aa) sequence identity with mouse and rat IGFBP1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-14
