Mouse IGFBP-1 ELISA Kit PicoKine™

Mouse IGFBP-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Igfbp1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK0383
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse IGFBP-1 ELISA Kit PicoKine™

See all IGFBP-1 products

SKU/Catalog Number

EK0383

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IGFBP-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Igfbp1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse IGFBP-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0383)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A26-N272

Sensitivity

<3 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0383 is reactive to Igfbp1 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0383 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0383-CAP Anti-Mouse Igfbp1 Pre-coated 96-well strip microplate 1
EK0383-ST Mouse Igfbp1 Standard 2 vials, 10 ng/tube
EK0383-DA Mouse Igfbp1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.1280.1880.2400.3310.5810.9411.4981.909

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IGFBP-1, Dilution ratio of 1:10, concentration in serum is 2737-4530pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IGFBP-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8828186590267783
Standard deviation6.614.7738.067.9219.4936.8
CV (%)7.5%5.4%4.4%8.8%7.3%4.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IGFBP-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 188848794883.634.1%
Sample 228131126029728718.976.6%
Sample 386590381794288146.245.2%
*number of samples for each test n=16.

Gene/Protein Information For Igfbp1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P47876

Gene ID

16006

Gene Name

Igfbp1

Full Name

Insulin-like growth factor-binding protein 1

Weight

29.57kDa

Alternative Names

alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP-1; IBP1; IBP-1; IGF-binding protein 1; IGFBP1; IGFBP-1; IGF-BP25; insulin-like growth factor binding protein 1; insulin-like growth factor-binding protein 1; Placental protein 12; PP12AFBP Igfbp1|IGFBP|insulin-like growth factor binding protein 1|insulin-like growth factor-binding protein 1|IBP-1|IGF-binding protein 1|INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 1 PRECURSOR (IGFBP-1) (IBP-1) (IGF-BINDING PROTEIN 1)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Igfbp1, check out the Igfbp1 Infographic

Igfbp1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Igfbp1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0383 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Insulin-like growth factor binding protein-1 levels are increased in patients with IgA nephropathy

Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels

Zhang S, Zhao Y, Hu X, Liu Z, Chen X, Chen X, Du J. Mol Cell Endocrinol. 2013 Aug 25;376(1-2):33-42. Doi: 10.1016/J.Mce.2013.05.025. Epub 2013 Jun 6. Distinct Post-Transcriptional Regulation Of Igfbp1 Gene By Hypoxia In Lowland Mouse And Qinghai-T...

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8 Customer Q&As for Mouse IGFBP-1 ELISA Kit PicoKine™

Question

Q: Can IGFBP-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

E. Turner

Verified customer

Asked: 2020-10-12

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-10-12

Question

Q: What is the optimal O.D. value for IGFBP-1 ELISA kit? I used your IGFBP-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IGFBP-1 even though the O.D. values are not very high?

D. Dhawan

Verified customer

Asked: 2020-03-03

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IGFBP-1 ELISA kit to have sensitivity of 3pg/ml, that means the minimum amount of IGFBP-1 that can be declared/interpreted as positive by the above standard is 3pg/ml.

Boster Scientific Support

Answered: 2020-03-03

Question

One of our customers is interested in the Mouse IGFBP-1 PicoKine

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

The recommended mouse serum dilution range is 1:100-1:500.

Boster Scientific Support

Answered: 2019-12-11

Question

What is the recommended mouse serum dilution range for EK0383?

Verified customer

Asked: 2019-09-04

Answer

For the Mouse IGFBP-1 ELISA Kit PicoKine™ (EK0383), the recommended mouse serum dilution range is 1:100-1:500.

Boster Scientific Support

Answered: 2019-09-04

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

C. Gonzalez

Verified customer

Asked: 2019-04-16

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-04-16

Question

Q: how to analyze ELISA data? I have obtained IGFBP-1 level in plasma.

Verified Customer

Verified customer

Asked: 2019-03-10

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-03-10

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of IGFBP-1 in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

P. Baker

Verified customer

Asked: 2018-02-13

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IGFBP-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-02-13

Question

Was baculovirus used in the production of EK0383? Keywords: manufacturing, transfection

Verified Customer

Verified customer

Asked: 2016-12-05

Answer

Baculovirus was not used in the production of EK0383.

Boster Scientific Support

Answered: 2016-12-05

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