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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse IGFBP-1 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse IGFBP-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Igfbp1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IGFBP-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0383)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A26-N272
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0383 is reactive to Igfbp1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0383 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0383-CAP||Anti-Mouse Igfbp1 Pre-coated 96-well strip microplate||1|
|EK0383-ST||Mouse Igfbp1 Standard||2 vials, 10 ng/tube|
|EK0383-DA||Mouse Igfbp1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse IGFBP-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IGFBP-1, Dilution ratio of 1:10, concentration in serum is 2737-4530pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IGFBP-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IGFBP-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Igfbp1 (Source: Uniprot.Org, NCBI)
Insulin-like growth factor-binding protein 1
alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP-1; IBP1; IBP-1; IGF-binding protein 1; IGFBP1; IGFBP-1; IGF-BP25; insulin-like growth factor binding protein 1; insulin-like growth factor-binding protein 1; Placental protein 12; PP12AFBP Igfbp1|IGFBP|insulin-like growth factor binding protein 1|insulin-like growth factor-binding protein 1|IBP-1|IGF-binding protein 1|INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 1 PRECURSOR (IGFBP-1) (IBP-1) (IGF-BINDING PROTEIN 1)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Igfbp1, check out the Igfbp1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Igfbp1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0383 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Insulin-like growth factor binding protein-1 levels are increased in patients with IgA nephropathy
Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels
Zhang S, Zhao Y, Hu X, Liu Z, Chen X, Chen X, Du J. Mol Cell Endocrinol. 2013 Aug 25;376(1-2):33-42. Doi: 10.1016/J.Mce.2013.05.025. Epub 2013 Jun 6. Distinct Post-Transcriptional Regulation Of Igfbp1 Gene By Hypoxia In Lowland Mouse And Qinghai-T...
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