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Product Info Summary
| SKU: | A00888 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Gfi-1 Monoclonal Antibody
SKU/Catalog Number
A00888
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Gfi-1 Monoclonal Antibody catalog # A00888. Tested in WB, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gfi-1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00888)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5D7
Isotype
IgG
Immunogen
Purified recombinant fragment of human Gfi-1 expressed in E. Coli.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00888 is reactive to GFI1 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
45.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00888 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis using Gfi-1 Monoclonal Antibody against HEK293 (1) and GFI1-hIgGFc transfected HEK293 (2) cell lysate.
Specific Publications For Anti-Gfi-1 Monoclonal Antibody (A00888)
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3 Customer Q&As for Anti-Gfi-1 Monoclonal Antibody
Question
Do you have a BSA free version of anti-Gfi-1 Monoclonal antibody A00888 available?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Gfi-1 Monoclonal antibody A00888 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-01
Question
I see that the anti-Gfi-1 Monoclonal antibody A00888 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-03-22
Question
Would anti-Gfi-1 Monoclonal antibody A00888 work on dog WB with lung testis?
B. Carter
Verified customer
Asked: 2016-06-21
Answer
Our lab technicians have not validated anti-Gfi-1 Monoclonal antibody A00888 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Gfi-1 Monoclonal antibody A00888 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog lung testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-06-21
