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- Table of Contents
HES5 antibody (A05384) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 μg/ml.
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Anti-Transcription factor HES-5 HES5 Antibody
HES5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Transcription factor HES-5 HES5 Antibody
View all HES5 Antibodies
View all HES5 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
HES5 antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
HES5 antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5. The immunogen is located within the first 50 amino acids of HES5.
Cross-Reactivity
HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
18.2 kDa
Background of HES5
HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).
Cite This Product
Anti-Transcription factor HES-5 HES5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05384)
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Product Promise
We promise Anti-Transcription factor HES-5 HES5 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A05384
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026