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Product Info Summary
| SKU: | M00108 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Glucose 6 phosphate isomerase (GPI) Mouse Monoclonal Antibody [Clone ID: OTI5A11]
View all Glucose 6 phosphate isomerase Antibodies
SKU/Catalog Number
M00108
Size
100 µl
Description
Boster Bio Anti-GPI (Glucose 6 phosphate isomerase) mouse monoclonal antibody, clone OTI5A11 (formerly 5A11). Catalog# M00108. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glucose 6 phosphate isomerase (GPI) Mouse Monoclonal Antibody [Clone ID: OTI5A11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00108)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5A11
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human GPI (NP_000166) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00108 is reactive to GPI in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
63.147kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00108 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Flow cytometric Analysis of Jurkat cells
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Anti-GPI mouse monoclonal antibody (M00108) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPI.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPI.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPI monoclonal antibody.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and GPI-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-GPI monoclonal antibody M00108 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Protein Target Info & Infographic
Gene/Protein Information For GPI (Source: Uniprot.org, NCBI)
Gene Name
GPI
Full Name
Glucose-6-phosphate isomerase
Weight
63.147kDa
Superfamily
GPI family
Alternative Names
AMFGNPI; Autocrine motility factor; DKFZp686C13233; EC 5.3.1.9; glucose phosphate isomerase; glucose-6-phosphate isomerase; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; NLKSA36; oxoisomerase; PGI; PHI; Phosphoglucose isomerase; phosphohexomutase; Phosphohexose isomerase; phosphosaccharomutase; SA-36; Sperm antigen 36; sperm antigen-36 GPI AMF, GNPI, NLK, PGI, PHI, SA-36, SA36 glucose-6-phosphate isomerase glucose-6-phosphate isomerase|autocrine motility factor|hexose monophosphate isomerase|hexosephosphate isomerase|neuroleukin|oxoisomerase|phosphoglucose isomerase|phosphohexomutase|phosphohexose isomerase|phosphosaccharomutase|sperm -36
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPI, check out the GPI Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glucose 6 phosphate isomerase (GPI) Mouse Monoclonal Antibody [Clone ID: OTI5A11] (M00108)
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