Anti-Annexin A2/ANXA2 Antibody

Rabbit Polyclonal Annexin A2/ANXA2 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

SKU PA1348
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-Annexin A2/ANXA2 Antibody
See all ANXA2 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1348
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit Polyclonal Annexin A2/ANXA2 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat. Size: 100μg/vial.
Cite This Product Anti-Annexin A2/ANXA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1348)
Specificity Anti-Annexin A2/ANXA2 Antibody (PA1348) reacts with Human, Mouse, Rat ANXA2, in native form and recombinant. Superfamily members of ANXA2 are not reactive to PA1348.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-Annexin A2/ANXA2 Antibody (PA1348) was raised against A synthetic peptide corresponding to a sequence in the middle region of human Annexin A2(121-141aa RAEDGSVIDYELIDQDARDLY), different from the rat sequence by one amino acid..
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-Annexin A2/ANXA2 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-Annexin A2/ANXA2 Antibody (PA1348).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/pa1348 1 WB anti annexin a2 antibody.jpg

Anti-Annexin A2 antibody, PA1348, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Ovary Tissue Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: A549 Cell Lysate
Lane 7: JURKAT Cell Lysate

/antibody/pa1348 2 IHC anti annexin a2 antibody.jpg

Anti-Annexin A2 antibody, PA1348, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

/p/a/pa1348 3.jpg

Figure 3. Western blot analysis of Annexin A2 using anti-Annexin A2 antibody (PA1348).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates
Lane 2: mouse NIH/3T3 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A2 antigen affinity purified polyclonal antibody (Catalog # PA1348) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A2 at approximately 36KD. The expected band size for Annexin A2 is at 39KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P07355
Gene Name ANXA2
Protein Name Annexin A2
Alternative Names Annexin A2;Annexin II;Annexin-2;Calpactin I heavy chain;Calpactin-1 heavy chain;Chromobindin-8;Lipocortin II;Placental anticoagulant protein IV;PAP-IV;Protein I;p36;ANXA2;ANX2, ANX2L4, CAL1H, LPC2D;Show more
Subcellular Localization Secreted, extracellular space, extracellular matrix, basement membrane . Melanosome . In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
Molecular Weight 38604 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed:18799458, PubMed:24808179, PubMed:22848640). .
Background Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. The ANXA2 gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. This protein is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. Richard et al.(1994) presented an integration of the physical, expression, and genetic maps of human chromosome 15. They placed the ANXA2 gene in their region IV, i.e., 15q21-q22, thus confirming the previous localization.

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Publishing with Anti-Annexin A2/ANXA2 Antibody (PA1348)? Please let us know so we can cite the reference in this product datasheet. Email us at support@bosterbio.com.

Order Product (PA1348)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

Ships next business day.

Troubleshooting

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Publications

Zhu J, Zou W, Jia G, Zhou H, Hu Y, Peng M, Chen H, Jin M. J Proteomics. 2012 Mar 16;75(6):1732-41. Doi: 10.1016/J.Jprot.2011.12.012. Epub 2011 Dec 20. Analysis Of Cellular Proteome Alterations In Porcine Alveolar Macrophage Cells Infected With 200...

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to annexin a2 antibody, annexin ii antibody, annexin-2 antibody