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Breast Cancer Regulation
Rabbit Polyclonal Annexin A2/ANXA2 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.
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Our Boster Quality Guarantee for Anti-Annexin A2/ANXA2 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
The following reagents are used to generate the images below for Anti-Annexin A2/ANXA2 Antibody (PA1348).
Anti-Annexin A2 antibody, PA1348, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Rat Lung Tissue LysateLane 3: Rat Ovary Tissue LysateLane 4: MCF-7 Cell LysateLane 5: SMMC Cell LysateLane 6: A549 Cell LysateLane 7: JURKAT Cell Lysate
Anti-Annexin A2 antibody, PA1348, IHC(P)IHC(P): Human Intestinal Cancer Tissue
Figure 3. Western blot analysis of Annexin A2 using anti-Annexin A2 antibody (PA1348). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates Lane 2: mouse NIH/3T3 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A2 antigen affinity purified polyclonal antibody (Catalog # PA1348) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A2 at approximately 36KD. The expected band size for Annexin A2 is at 39KD.
*if product is indicated to react with multiple species, protein info is based on the human gene.
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