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- Table of Contents
GSTP1 antibody (PA1040) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of GSTP1 using anti-GSTP1 antibody (PA1040).
GSTP1 was detected in a paraffin-embedded section of human highly differentiated adenocarcinoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTP1 Antibody (PA1040) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
GSTP1 was detected in a paraffin-embedded section of human highly differentiated adenocarcinoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTP1 Antibody (PA1040) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-GST3/GST pi/GSTP1 Antibody Picoband®
GSTP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
GSTP1 antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human Jurkat whole cell, rat lung tissue, mouse lung tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GSTP1 antibody was positively detected by IHC in:
human highly differentiated adenocarcinoma of the colon tissue, human moderately differentiated adenocarcinoma of the cervical canal tissue, human ovarian cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GST3/GST pi/GSTP1 Antibody Picoband®
View all GSTP1 Antibodies
View all GSTP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GST3, different from the related mouse sequence by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated Molecular Weight
23.4 kDa
Background of GSTP1
Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11 .Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.
Cite This Product
Anti-GST3/GST pi/GSTP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1040)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
93%
High Homology
Mouse
86%
Moderate Homology
Sheep
86%
Moderate Homology
Dog
86%
Moderate Homology
Cow
79%
Moderate Homology
Rat
71%
Moderate Homology
Rabbit
71%
Moderate Homology
Zebrafish
64%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GSTP1 antibody
Immunohistochemistry (IHC) protocol for GSTP1 antibody
Publications
Application:
Species:
Chemoresistance in Malignant Intracranial Tumors: Longer Survival with Negative MDR1 Expression.
Published: 2020-11-12
Journal: Turkish Neurosurgery
Application: IHC
Impact Factor: 0.96
Species: Human
PMID: 33978213
Expressions of glutathione S-transferase alpha, mu, pi, and theta in the skin samples of patients with acne rosacea
Published: 2020-05-20
Journal: Journal of Cosmetic Dermatology
Application: IHC
Impact Factor: 1.62
Species: Human
PMID: 32433803
Glutathione S-Transferases and Cytochrome P450 Enzyme Expression in Patients with Intracranial Tumors: Preliminary Report of 55 Patients
Published: 2018-10-15
Journal: MEDICAL PRINCIPLES AND PRACTICE
Application: IHC
Impact Factor: 1.54
Species: Human
PMID: 30321868
Adenosine Promotes the Recovery of Mice from the Cuprizone-Induced Behavioral and Morphological Changes while Effecting on Microglia and Inflammatory Cytokines in the Brain
Published: 2018-08-01
Journal: Journal of Neuroimmune Pharmacology
Application: IHC
Impact Factor: 3.66
Species: Mouse
PMID: 30069711
N-acetylcysteine attenuates the cuprizone-induced behavioral changes and oligodendrocyte loss in male C57BL/7 mice via its anti-inflammation actions
Published: 2017-11-08
Journal: JOURNAL OF NEUROSCIENCE RESEARCH
Application: IHC
Impact Factor: 2.48
Species: Mouse
PMID: 29114910
Changes in proinflammatory cytokines and white matter in chronically stressed rats
Published: 2015-03-06
Journal: Neuropsychiatric Disease and Treatment
Application: IF
Impact Factor: 1.74
Species: Rat
PMID: 25834438
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Product Promise
We promise Anti-GST3/GST pi/GSTP1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1040
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026