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Product Info Summary
| SKU: | A01495 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Hax1a Antibody
SKU/Catalog Number
A01495
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hax1a Antibody (Catalog # A01495). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Hax1a antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Hax1a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01495)
Host
Rabbit
Contents
Hax1a Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Hax1a antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human Hax1a. The immunogen is located within the first 50 amino acids of Hax1a.
Cross-reactivity
At least four isoforms of Hax1 are known to exist. This antibody is expected to recognize the longest isoform (Hax1a) as well as the shortest.
Reactive Species
A01495 is reactive to HAX1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
31.6 kDa
Background of HAX1
The HS-1 associated protein X-1 (Hax1) was initially identified in a yeast two-hybrid assay on the basis of its ability to bind to the hemapoietic cell-specific protein 1 (HS-1). Hax1 possesses anti-apoptotic activity and is structurally related to Bcl-2 family members, including the presence of BH1- and BH2-like domains. It has recently been shown to interact with HIV viral protein R (Vpr), a protein required for viral pathogenesis of HIV and linked to T-cell apoptosis through activation of caspases 3 and 9. Other studies indicate that Hax1-mediated processing of HtrA2 (also known as Omi) by the mitochondrial protease PARL allows survival of lymphocytes and neurons when cytokines are limiting.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01495 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Hax1a antibody can be used for detection of Hax1A by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Hax1a in human brain tissue lysate with Hax1a antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-Hax1a Antibody (A01495)
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