Anti-HDAC-1 (C-terminus) Antibody

Product Name

Anti-HDAC-1 (C-terminus) Antibody

View all HDAC1 Antibodies

SKU/Catalog Number

A00256-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-HDAC-1 (C-terminus) Antibody (Catalog # A00256-1). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-HDAC-1 (C-terminus) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00256-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 450-482 of Human HDAC-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00256-1 is reactive to HDAC1 in Human

Applications

A00256-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

55.103kDa

Background of HDAC1

HDAC-1 antibody recognizes HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 1:200 - 1:1,000
IF Microscopy: 10ug / ml
WB: 1:1,000 - 1:5,000

Validation Images & Assay Conditions

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HDAC-1 was detected in paraffin-embedded sections of human prostate cancer tissue using rabbit anti-HDAC-1 Antigen Affinity purified polyclonal antibody (Catalog # A00256-1) at 1:500. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 precipitated purple with blue counterstain. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.

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Western blot analysis of HDAC-1 expression in 293 whole cell lysate. HDAC-1 at 65KD was detected using rabbit anti-HDAC-1 Antigen Affinity purified polyclonal antibody (Catalog # A00256-1)at 1:3500.

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HDAC-1 was detected in paraffin-embedded sections of human lung tissue using rabbit anti-HDAC-1 Antigen Affinity purified polyclonal antibody (Catalog # A00256-1) at 10 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

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Immunofluorescence Microscopy of Rabbit Anti-HDAC-1 antibody. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10

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HDAC-1 was detected in immunocytochemical sections of A431 cells using rabbit anti-HDAC-1 Antigen Affinity purified polyclonal antibody (Catalog # A00256-1) at 0.4

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Western blot analysis of HDAC-1 expression in LNCaP prostate cancer cells. HDAC-1 at 55KD was detected using rabbit anti-HDAC-1 Antigen Affinity purified polyclonal antibody (Catalog # A00256-1) at 1:1000.

Gene/Protein Information For HDAC1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.5.1.98; GON-10; HD1; HD1DKFZp686H12203; Histone Deacetylase 1; KDAC1; reduced potassium dependency, yeast homolog-like 1; RPD3L1; RPD3L1RPD3 HDAC1 GON-10, HD1, KDAC1, RPD3, RPD3L1 histone deacetylase 1 histone deacetylase 1|reduced potassium dependency, yeast homolog-like 1

For more info on HDAC1, check out the HDAC1 Infographic

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In this infographic, you will see the following information for HDAC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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