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Product Info Summary
| SKU: | M00256 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HDAC1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00256
BM4045 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HDAC1 Rabbit Monoclonal Antibody catalog # M00256. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00256)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AIB-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HDAC1
Reactive Species
M00256 is reactive to HDAC1 in Human, Mouse, Rat
Observed Molecular Weight
55-60 kDa
Calculated molecular weight
55.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00256 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human testis tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of HDAC1 expression in (1) C6 cell lysate; (2) NIH/3T3 cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human testis, using HDAC1 Antibody.
Specific Publications For Anti-HDAC1 Rabbit Monoclonal Antibody (M00256)
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3 Customer Q&As for Anti-HDAC1 Rabbit Monoclonal Antibody
Question
I see that the anti-HDAC1 Rabbit Monoclonal antibody M00256 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-29
Question
We are currently using anti-HDAC1 Rabbit Monoclonal antibody M00256 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The anti-HDAC1 Rabbit Monoclonal antibody (M00256) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-03
Question
Does anti-HDAC1 Rabbit Monoclonal antibody M00256 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
According to the expression profile of leukemic t-cell, HDAC1 is highly expressed in leukemic t-cell. So, it is likely that anti-HDAC1 Rabbit Monoclonal antibody M00256 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2018-12-10
