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Product Info Summary
| SKU: | A00256 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-HDAC1 (E468) Antibody
SKU/Catalog Number
A00256
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HDAC1 (E468) Antibody catalog # A00256. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC1 (E468) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00256)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 435-486 of Human HDAC1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00256 is reactive to HDAC1 in Human, Mouse, Rat
Calculated molecular weight
55.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00256 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of HDAC1 (E468) polyclonal antibody at 1:500 dillution Lane1:HEK293T whole cell lysate(40μg) Lane2:Hela whole cell lysate(40μg) Lane3:The Kidney tissue lysate of Mouse(40μg) Lane4:The Kidney tissue lysate of Rat(40μg) Lane5:The Heart tissue lysate of Mouse(40μg) Lane6:The Heart tissue lysate of Rat(40μg)
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Immunohistochemistry (IHC) analyzes of HDAC1 (E468) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Immunoprecipitation of L02 cell lysate using HDAC1 (E468) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using HDAC1 (E468) pAb #BS1160.“↑”（supernatant）; “↓”（deposition）
Specific Publications For Anti-HDAC1 (E468) Antibody (A00256)
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