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Product Info Summary
| SKU: | M00253 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody
View all HO-1/HMOX1/HSP32 Antibodies
SKU/Catalog Number
M00253
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody catalog # M00253. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00253)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEG-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Heme Oxygenase 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00253 is reactive to HMOX1 in Human, Mouse
Applications
M00253 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Observed Molecular Weight
80 kDa
Calculated molecular weight
32.819kDa
Background of HO-1/HMOX1/HSP32
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of Heme Oxygenase 1 in mouse spleen lysate.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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IHC analysis of Heme Oxygenase 1 using anti-Heme Oxygenase 1 antibody (M00253).
Heme Oxygenase 1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Heme Oxygenase 1 Antibody (M00253) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HMOX1 (Source: Uniprot.org, NCBI)
Gene Name
HMOX1
Full Name
Heme oxygenase 1
Weight
32.819kDa
Superfamily
heme oxygenase family
Alternative Names
bK286B10; EC 1.14.99.3; heat shock protein, 32-kD; heme oxygenase (decycling) 1; HMOX1; HO; HO1; HO-1; HO-1heme oxygenase 1; HSP32 HMOX1 HMOX1D, HO-1, HSP32, bK286B10 heme oxygenase 1 heme oxygenase 1|heat shock protein, 32-kD|heme oxygenase (decycling) 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HMOX1, check out the HMOX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HMOX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody (M00253)
Hello CJ!
M00253 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li D,Bai X,Jiang Y,Cheng Y.Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway.Brain Res Bull.2020 Dec 28;168:25-35.doi:10.1016/j.brainresbull.2020.12.009.Epub ahead of print
Species: Mouse
M00253 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS, DILUTION:1:200
Role of heme oxygenase-1/carbon monoxide system in pulmonary ischemia-reperfusion injury
Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression
Evidence for a therapeutic effect of Braintone on ischemic brain damage
Upregulation of heat shock protein 32 in Sertoli cells alleviates the impairments caused by heat shock-induced apoptosis in mouse testis
Comparison of the Effects of Propofol and Midazolam on Inflammation and Oxidase Stress in Children with Congenital Heart Disease Undergoing Cardiac Surgery
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Mo L, Yang C, Gu M, Zheng D, Lin L, Wang X, Lan A, Hu F, Feng J. Int J Mol Med. 2012 Aug;30(2):314-20. Doi: 10.3892/Ijmm.2012.1002. Epub 2012 May 14. Pi3K/Akt Signaling Pathway-Induced Heme Oxygenase-1 Upregulation Mediates The Adaptiv...
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody
Question
Our lab used your anti-Heme Oxygenase 1 Rabbit Monoclonal antibody for IP on layer of synovial tissue a few months ago. I am using mouse, and We want to use the antibody for IHC next. We want examining layer of synovial tissue as well as foreskin in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
V. Mitchell
Verified customer
Asked: 2019-10-23
Answer
I viewed the website and datasheets of our anti-Heme Oxygenase 1 Rabbit Monoclonal antibody and it appears that M00253 has been validated on mouse in both IP and IHC. Thus M00253 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-23
Question
Our team were content with the WB result of your anti-Heme Oxygenase 1 Rabbit Monoclonal antibody. However we have been able to see positive staining in foreskin microsome. using this antibody. Is that expected? Could you tell me where is HMOX1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
From what I have seen in literature, foreskin does express HMOX1. Generally HMOX1 expresses in microsome. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585
Boster Scientific Support
Answered: 2019-09-25
Question
We are currently using anti-Heme Oxygenase 1 Rabbit Monoclonal antibody M00253 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on goat tissues as well?
G. Williams
Verified customer
Asked: 2018-12-10
Answer
The anti-Heme Oxygenase 1 Rabbit Monoclonal antibody (M00253) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-10
Question
We have observed staining in mouse layer of synovial tissue. Do you have any suggestions? Is anti-Heme Oxygenase 1 Rabbit Monoclonal antibody supposed to stain layer of synovial tissue positively?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
From literature layer of synovial tissue does express HMOX1. From Uniprot.org, HMOX1 is expressed in layer of synovial tissue, foreskin, cervix carcinoma, among other tissues. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585
Boster Scientific Support
Answered: 2018-07-20
