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Product Info Summary
| SKU: | M00253 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00253
BM4010 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody catalog # M00253. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00253)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEG-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Heme Oxygenase 1
Reactive Species
M00253 is reactive to HMOX1 in Human, Mouse
Observed Molecular Weight
33 kDa
Calculated molecular weight
32.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00253 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
FC 1:20
Positive Control
WB:human U87-MG whole cell, human A549 whole cell, human 293T whole cell, human Hela whole cell
IHC: human spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of HO-1 using anti-HO-1 antibody (M00253).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87-MG whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HO-1 antigen affinity purified monoclonal antibody (M00253) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HO-1 at approximately 33 kDa. The expected band size for HO-1 is at 33 kDa.
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SXD remarkably affected the ferroptosis-related markers in CoCl 2 -induced hypoxic H9c2 cells. (A–D) mRNA expressions of ACSL4, GPX4, DPP4, and HMOX1 in different groups, separately. (E) Protein expressions of ACSL4, GPX4, DPP4, and HMOX1 in different groups. *P < 0.05, **P < 0.01, and ***P < 0.001.
Index in PubMed under a CC BY license. PMID: 40365322
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Molecular docking results. (A) Structure of quercetin. (B, C) Molecular docking of quercetin with DPP4 (B) and HMOX1 (C) . (D) Results of the CETSA assay.
Index in PubMed under a CC BY license. PMID: 40365322
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IHC analysis of Heme Oxygenase 1 using anti-Heme Oxygenase 1 antibody (M00253).
Heme Oxygenase 1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Heme Oxygenase 1 Antibody (M00253) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody (M00253)
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4 Customer Q&As for Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody
Question
Our lab used your anti-Heme Oxygenase 1 Rabbit Monoclonal antibody for IP on layer of synovial tissue a few months ago. I am using mouse, and We want to use the antibody for IHC next. We want examining layer of synovial tissue as well as foreskin in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
V. Mitchell
Verified customer
Asked: 2019-10-23
Answer
I viewed the website and datasheets of our anti-Heme Oxygenase 1 Rabbit Monoclonal antibody and it appears that M00253 has been validated on mouse in both IP and IHC. Thus M00253 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-23
Question
Our team were content with the WB result of your anti-Heme Oxygenase 1 Rabbit Monoclonal antibody. However we have been able to see positive staining in foreskin microsome. using this antibody. Is that expected? Could you tell me where is HMOX1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
From what I have seen in literature, foreskin does express HMOX1. Generally HMOX1 expresses in microsome. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585
Boster Scientific Support
Answered: 2019-09-25
Question
We are currently using anti-Heme Oxygenase 1 Rabbit Monoclonal antibody M00253 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on goat tissues as well?
G. Williams
Verified customer
Asked: 2018-12-10
Answer
The anti-Heme Oxygenase 1 Rabbit Monoclonal antibody (M00253) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-10
Question
We have observed staining in mouse layer of synovial tissue. Do you have any suggestions? Is anti-Heme Oxygenase 1 Rabbit Monoclonal antibody supposed to stain layer of synovial tissue positively?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
From literature layer of synovial tissue does express HMOX1. From Uniprot.org, HMOX1 is expressed in layer of synovial tissue, foreskin, cervix carcinoma, among other tissues. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585
Boster Scientific Support
Answered: 2018-07-20
