Skip to the beginning of the images gallery

HMOX1 antibody (M00253) performs well in Flow Cytometry, IP, IHC, WB and shows reactivity with Human, Mouse.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 23 Publications

Datasheet MSDS Request COA

m00253 ho 1 primary antibodies ihc testing 2

View All Images (4)

IHC analysis of Heme Oxygenase 1 using anti-Heme Oxygenase 1 antibody (M00253).
Heme Oxygenase 1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Heme Oxygenase 1 Antibody (M00253) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody

HMOX1 antibody

23 Publications

Reactive Species	Host	Application
Human, Mouse	Rabbit	Flow Cytometry, IP, IHC, WB

Tested Applications

HMOX1 antibody was positively detected by WB in:

human U87-MG whole cell, human A549 whole cell, human 293T whole cell, human Hela whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

HMOX1 antibody was positively detected by IHC in:

human spleen tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Mouse

Recommend

Product Information

Product Name

Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody
View all HMOX1 Antibodies

Form

Liquid

Uniprot

P09601

Gene ID

3162

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

AEG-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Heme Oxygenase 1

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

33 kDa

Calculated Molecular Weight

32.8 kDa

Cite This Product

Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00253)

Protocols

Western Blot protocol for HMOX1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for HMOX1 antibody

Review Protocol

Publications

Vanillic acid protects vertebral endplate chondrocytes against oxidative stress and inflammation via Nrf-2/HO-1 pathway

Published: 2026-07-23

Journal: EUROPEAN JOURNAL OF PHARMACOLOGY

Application: WB

Impact Factor: 5.70

Species: Mouse

PMID: 42492879

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody

HMOX1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Vanillic acid protects vertebral endplate chondrocytes against oxidative stress and inflammation via Nrf-2/HO-1 pathway

Tetrastigma hemsleyanum Diels & Gilg ameliorates alcohol-associated liver disease by modulating the gut-liver axis and ferroptosis

Hydroxytyrosol Enhances the Nrf2/HO-1 Signalling Pathway to Inhibit Oxidative Stress and Apoptosis and Improve Premature Ovarian Insufficiency In Vitro and In Vivo

Yangjing Shugan decoction targets the Sirt1/Nrf2 antioxidant pathway and gut microbiota-metabolite axis to protect against premature ovarian failure

Molecular Expression Differences in Specific Blood Mononuclear Cell‐Types Identify Patients With AL Amyloidosis

Signalling Network Analysis of Blood Mononuclear Cells From Clinical Samples by Bivariate Correlation

Quercetin in Shengxian Decoction exhibits anti-ferroptosis protective roles in a myocardial infarction model via targeting DPP4/ HMOX1, based on network pharmacology and molecular docking

Taraxasterol attenuates zearalenone-induced kidney damage in mice by modulating oxidative stress and endoplasmic reticulum stress

Baicalein attenuates oxidative damage in mice haematopoietic cells through regulation of PDGFRβ

Cell-Cycle-related Protein Centromere Protein F Deficiency Inhibits Cervical Cancer Cell Growth by Inducing Ferroptosis Via Nrf2 Inactivation

RNA-seq combined network pharmacology reveals that Fu-Gan-Wan (FGW) inhibits liver fibrosis via NF-κB/CCL2/CCR2 and lipid peroxidation via Nrf2/HMOX1 signaling pathway

Nrf2 protects against cartilage endplate degeneration through inhibiting NCOA4‑mediated ferritinophagy

Activation of the Nrf-2 pathway by pinocembrin safeguards vertebral endplate chondrocytes against apoptosis and degeneration caused by oxidative stress

Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments

Astaxanthin suppresses oxidative stress and calcification in vertebral cartilage endplate via activating Nrf-2/HO-1 signaling pathway

PTEN inhibitor VO-OHpic protects endplate chondrocytes against apoptosis and calcification via activating Nrf-2 signaling pathway

Icariin protects vertebral endplate chondrocytes against apoptosis and degeneration via activating Nrf-2/HO-1 pathway

The structural characteristic of bamboo shoot shell polysaccharides extracted using ultrasound-assisted phosphotungstic acid hydrolysis and its protection against cell oxidative injury

Vitamin D3 Protects Mice from Diquat-Induced Oxidative Stress through the NF-κB/Nrf2/HO-1 Signaling Pathway.

Hepatocellular iNOS protects liver from NASH through Nrf2-dependent activation of HO-1

miR-155 mediates arsenic trioxide resistance by activating Nrf2 and suppressing apoptosis in lung cancer cells

Nephroprotective Effects of Saponins from Leaves of Panax quinquefolius against Cisplatin-Induced Acute Kidney Injury

Influence of heme oxygenase-1 expression on immune liver fibrosis induced by cobalt protoporphyrin in rats

Review

Have you used Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody?

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality