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- Table of Contents
4 Citations 10 Q&As
25 Citations 16 Q&As
8 Citations 4 Q&As
19 Citations 5 Q&As
1 Citations
Facts about Heme oxygenase 1.
Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Human | |
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Gene Name: | HMOX1 |
Uniprot: | P09601 |
Entrez: | 3162 |
Belongs to: |
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heme oxygenase family |
bK286B10; EC 1.14.99.3; heat shock protein, 32-kD; heme oxygenase (decycling) 1; HMOX1; HO; HO1; HO-1; HO-1heme oxygenase 1; HSP32
Mass (kDA):
32.819 kDA
Human | |
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Location: | 22q12.3 |
Sequence: | 22; NC_000022.11 (35381096..35394207) |
Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).
Microsome. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
Boster Bio has a protocol that can be used to detect HMOX1 in samples. This method can be applied in a variety of situations, from prescreening to validation. To find out more about Boster Bio's HMOX1 and HMOX2 markers, read this article.
Boster Bio HMOX1 is a universal primer that detects heme oxygenase-1 expression. This enzyme is involved with the oxidation hemethat is a precursor to hemoglobin. The process of oxidation produces carbon monoxide, free iron and biliverdin. All of these play cytoprotective roles in the body. HMOX1 and HO-1 catalytic compounds are also associated with anti-apoptotic and antioxidant properties.
This primer set recognizes the p65 functional control domain as an internal control. Other activation domains can be used, leading to stronger ATFs. In addition, repressors could be used to stop the expression of HMOX1 and, thus, reduce the oxidative stress. The Boster Bio HMOX1PCR kit is highly recommended. It can also identify genes such as p53.
The Boster Bio Anti-Ho-1 Monoclonal Antibody (M00253-2) was tested for use in human cells by using negative and positive samples. The specificity and affinity of the antibody were confirmed by a renowned laboratory. Boster also provides product credits to the first scientists who review its products, thereby increasing its chances of receiving positive reviews. With this antibody, you'll be able test your hypothesis quickly and accurately.
The Boster Bio HMOX1 PCR kit is the perfect option for anyone looking for research antibodies for a number of fields. UCSF researcher Dr. Nevan Krogan has been researching COVID-19, the virus that causes. Boster Bio HMOX1 PCR kits enable researchers to study the protein spike that interacts with ACE2 receptor. The TMPRSS2 enzyme primes ace2 receptors, thereby allowing for the detection of a particular protein.
Boster Bio HMOX1 ELSA kits offer high sensitivity and specificity. Each 96-well kit delivers results within less than a day. Boster Bio HMOX1 ELISA kits are available in the 96 well and 96 test formats. Boster Bio ELISA kits contain antibodies that are validated for use in IHC, WB, and Flow tests. Boster Bio antibodies are tested against panels of more than 250 cell lines and tissues to ensure high affinity.
This ELISA test measures HMOX1 expression in human and animal samples. HMOX1 is part of the hemolytic enzyme family that catalyzes heme degradation. It is membrane-bound and cleaves an alpha-methene heme ring. It promotes pro-inflammatory cytokines secretion and hinders the maturation of the immune effector cells and increases production of anti-inflammatory.
It is a highly targeted therapeutic gene that has received much attention as a target. Numerous physiological and pathological processes are involved in the gene's involvement as have been studies. The increased expression in sarcomas and tumors is indicative of a wide range of inflammation-related disorders. By taking measurements of HMOX1, ELISA tests can help researchers determine the extent to which a drug is effective in treating these diseases.
Boster Bio HMOX1 ELSA kits have been used to analyze the ileal tissue samples for more than 15 years. Its use in human research has made it a standard and is extensively utilized in clinical trials. The company is located in Wuhan, Hubei, China. Boster Bio HMOX1 ELISA Kit offers a wide variety of test kits to test animals, plants and human immune reactions.
The HMOX1 marker can be used to detect the amount of iron in adipose tissues. Metabolic disturbances can be caused by excess iron levels in adipose tissues. Iron may cause obesity-related adipose disorders in obese individuals. Moreover, iron may contribute to the development of obesity-related illnesses like insulin resistance. Therefore, it is important to understand how iron affects adipose tissue.
ATF with NLS significantly increased HMOX1 expression in comparison to ATF without NLS. These results are in line with the expectations. The expression of reporter genes was upregulated by AZP, HMOX1 enhancer specific ATF and p65 functional domain. The results were in line with the expectations of the authors. The experiments were conducted in ECV304 cells, which were a convenient cell line to cultivate.
This marker is now an increasingly used tool for detecting obesity. Numerous studies have demonstrated the role played by HMOX1 in the regulation of adipogenesis and iron accumulation. The presence of HMOX1 in adipocytes has also been associated with increased levels of adiponectin as well as better glucose tolerance. Although it isn't clear if the HMOX1 gene expression can be used to determine obesity, it has been found in obese individuals as well as those who are not obese.
Recent studies have revealed that HMOX1 expression is associated with various pregnancy-related conditions, including miscarriage PE and endothelial diseases. However, HMOX1 expression is also decreased in a large subset of PE-DBMSCs. Although this marker hasn't been used to diagnose pregnancy disorders however, it is a promising candidate for predicting a positive pregnancy outcome in clinical studies.
Incredibly, HMOX1 is found in all tissues, including blood. The levels are low in the majority of tissues, and are primarily regulated by iron, oxidative stress and inflammation. HMOX1 activity and expression are directly related to mRNA levels as well as protein expression directly correlated with the severity of the disease. Apart from its potential function in predicting the occurrence of cancer, HMOX1 is also implicated in the development of PE. This suggests that treatment which targets the enzyme could be beneficial in this challenging condition.
The rate of degradation of heme is controlled by the HMOX1 gene. This gene is vital for the regulation of heme degradation. If it's not present, severe endothelial injury can be observed. The levels of von Willebrand factor, thrombomodulin, and thrombomodulin are high. Further research is needed to determine if the polymorphisms can be functional. The amplification results indicate that HMOX1 polymorphisms might have functional implications.
HMOX1 is widely identified as a sensitive indicator of oxidative stress. HMOX1 could be a great reporter gene to track this stress response by using hiPSCs. Future research could use this reporter gene to study and measure the increased activity of HMOX1 in response to chemical-induced stress. This article describes the uses and potential applications of this marker. We also describe an innovative approach that involves the use of fluorescent HMOX1 reporter hiPSC cells.
The first step was to design three different RNAs that target HMOX1. The gRNAs were introduced into cells using the appropriate sites, generating overlaid amplicons that extend across the entire inserted sequence. We then made three distinct HMOX1-targeting and RNAs and examined them in the laboratory. The HMOX1 mRNA was sensitive to DNA methylation and interference with RNA.
Then, we isolated genomic DNA from human peripheral blood leucocytes. We confirmed HbS status by performing a Covalent Reverse Dot Blot method. Based on the presumptive functional implications, we selected 3 polymorphisms within the HMOX1 genome. rs3074372 (GT)n repeats were identified using fluorescently labelled primers. The PCR products were loaded onto an ABI genetic analyser3130XL.
This enzyme breaks down heme, which gives rise to free iron, Bilirubin and other molecule. It regulates apoptosis. This enzyme is present in two isoforms, HMOX1 and HMOX2. The two are co-expressed in the brain and the endothelium vascular. Several stimuli are required to increase the activity of HMOX1 and the protein.
HMOX1 expression has been linked to recurrent miscarriages, as well as the delay in intrauterine development. While it's not clear whether HMOX1 deficiency is directly related with these conditions, it has been demonstrated that it could play a role in preventing these diseases. These findings need to be confirmed and confirmed clinically.
PMID: 3345742 by Yoshida T., et al. Human heme oxygenase cDNA and induction of its mRNA by hemin.
PMID: 2911585 by Keyse S.M., et al. Heme oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide, and sodium arsenite.
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