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- Table of Contents
AQP5 antibody (A03085) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of Aquaporin 5 using anti-Aquaporin 5 antibody (A03085).
Aquaporin 5 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Aquaporin 5 Antibody (A03085) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Aquaporin 5 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Aquaporin 5 Antibody (A03085) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-Aquaporin 5/AQP5 Antibody Picoband®
AQP5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
AQP5 antibody was positively detected by WB in:
rat lung tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AQP5 antibody was positively detected by IHC in:
mouse lung tissue, rat lung tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Human
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Aquaporin 5/AQP5 Antibody Picoband®
View all AQP5 Antibodies
View all AQP5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 5, which shares 71.7% and 73% amino acid (aa) sequence identity with mouse and rat Aquaporin 5, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated Molecular Weight
28.3 kDa
Background of AQP5
Aquaporin 5, also known as AQP5, is a water channel protein. The aquaporins (AQPs) are a family of more than 10 homologous water transporting proteins expressed in many mammalian epithelia and endothelia. At least five AQPs are expressed in the eye: AQP0 (MIP) in lens fiber, AQP1 in cornea endothelium, ciliary and lens epithelia and trabecular meshwork, AQP3 in conjunctiva, AQP4 in ciliary epithelium and retinal Müller cells, and AQP5 in corneal and lacrimal gland epithelia. Among the seven human aquaporins cloned to date (AQPs 0-6), genes encoding the four most closely related aquaporins (AQP0, AQP2, AQP5, and AQP6) have been mapped to chromosome band 12q13, suggesting an aquaporin family gene cluster at this locus. Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions.
Cite This Product
Anti-Aquaporin 5/AQP5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03085)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Sheep
82%
Moderate Homology
Cow
79%
Moderate Homology
Mouse
71%
Moderate Homology
Rat
71%
Moderate Homology
Rabbit
71%
Moderate Homology
Zebrafish
42%
Low homology
Drosophila
18%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for AQP5 antibody
Immunohistochemistry (IHC) protocol for AQP5 antibody
Publications
Application:
Species:
Effects of Mume Fructus extract on smoking-induced cough and TNBS-induced colitis in rats and its underlying mechanisms based on gut-lung body fluid metabolism
Published: 2025-09-13
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Rat
PMID: 40953772
Influenza virus uses mGluR2 as an endocytic receptor to enter cells
Published: 2024-06-07
Journal: Nature Microbiology
Application: IHC
Impact Factor: 20.50
Species: Mouse
PMID: 38849624
Review
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2 Reviews For Anti-Aquaporin 5/AQP5 Antibody Picoband®
In this Western blot (WB) experiment using Anti-AQP5 antibody (Cat# A03085) on MADB106 rat mammary carcinoma cells, AQP5 protein levels were significantly increased with agonist treatment and markedly reduced with inhibitor treatment.
| SKU | A03085 |
|---|---|
| Application | Western blot |
| Sample | MADB106 rat mammary carcinoma cells |
| Sample Processing Description | MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor |
| Other Reagents | RIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer |
| Primary Antibody | Aquaporin 5/AQP5 Antibody Picoband® |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG |
| Secondary Incubation | 1h at 37℃ |
| Detection | Substrate: ECL substrate, Imaging system:ChemiDoc MP |
| Results Summary | AQP5 (Aquaporin 5) is a transmembrane water channel protein that primarily regulates cellular water transport and plays a critical and complex role in tumor progression. In breast cancer, high AQP5 expression is often associated with increased tumor invasiveness, lymph node metastasis, and poor patient prognosis. In this experiment, AQP5 protein levels were markedly increased in cells treated with the agonist, whereas they were significantly reduced in cells treated with the inhibitor. |
Xinshuo Wang, Xi’an Jiaotong University
Verified customer
Submitted 2026-02-25
This antibody is highly efficient and specific, suitable for detecting AQP5 protein in rat colon by Western blot, with only minor nonspecific bands.
| SKU | A03085 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | Aquaporin 5/AQP5 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | This antibody is highly specific and efficient, suitable for detecting AQP5 protein in rat colon by Western blot, with only minimal nonspecific bands. |
Shiyu Zhang, LUTCM
Verified customer
Submitted 2025-12-30
Product Promise
We promise Anti-Aquaporin 5/AQP5 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A03085
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026