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- Table of Contents
Gstp1 antibody (A00394) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394).
GST3 / GST pi was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GST3 / GST pi Antibody (A00394) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
GST3 / GST pi was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GST3 / GST pi Antibody (A00394) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Designing a Western blot for GSTP1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the GSTP1 Western Blot GuideBundle Save
Anti-GST3/GST pi/Gstp1 Antibody Picoband®
Gstp1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
Gstp1 antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human Jurkat whole cell, rat liver tissue, rat stomach tissue, mouse liver tissue, mouse stomach tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Gstp1 antibody was positively detected by IHC in:
mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-GST3/GST pi/Gstp1 Antibody Picoband®
View all GSTP1 Antibodies
View all GSTP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse GST3 / GST pi recombinant protein (Position: P2-Q210).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
24kDa
Calculated Molecular Weight
23.6 kDa
Background of Gstp1
Glutathione S-transferases pi (GSTP1), also known as GST3, is an enzyme that in humans is encoded by the GSTP1 gene. This gene is mapped to 11q13.2. GSTP1 has 7 exons and 6 introns contained within approximately 2.8 kilobases. GSTP1 belongs to Glutathione S-transferases (GSTs) which are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 presents in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. What's more, GSTP1 is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Cite This Product
Anti-GST3/GST pi/Gstp1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00394)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
92%
High Homology
Dog
88%
Moderate Homology
Monkey
86%
Moderate Homology
Cow
86%
Moderate Homology
Human
85%
Moderate Homology
Rabbit
85%
Moderate Homology
Zebrafish
60%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Gstp1 antibody
Immunohistochemistry (IHC) protocol for Gstp1 antibody
Publications
Application:
Species:
Potential Role of GST-π in Lung Cancer Stem Cell Cisplatin Resistance.
Published: 2021-11-19
Journal: Biomed Research International
Application: WB
Impact Factor: 3.41
Species: Human
PMID: 34840986
Deep rTMS Mitigates Behavioral and Neuropathologic Anomalies in Cuprizone-Exposed Mice Through Reducing Microglial Proinflammatory Cytokines
Published: 2020-11-05
Journal: Frontiers in Integrative Neuroscience
Application: IHC
Impact Factor: 2.15
Species: Mouse
PMID: 33250722
Review
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Product Promise
We promise Anti-GST3/GST pi/Gstp1 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00394
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 28, 2026