Anti-Hex/HHEX Antibody

Rabbit Polyclonal Hex/HHEX Antibody. Validated in WB and tested in Human, Mouse, Rat.

SKU PA1326
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-Hex/HHEX Antibody
See all HHEX primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1326
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit Polyclonal Hex/HHEX Antibody. Validated in WB and tested in Human, Mouse, Rat. Size: 100μg/vial.
Cite This Product Anti-Hex/HHEX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1326)
Specificity Anti-Hex/HHEX Antibody (PA1326) reacts with Human, Mouse, Rat HHEX, in native form and recombinant. Superfamily members of HHEX are not reactive to PA1326.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-Hex/HHEX Antibody (PA1326) was raised against A synthetic peptide corresponding to a sequence at the N-terminus of human Hex(24-39aa QPAHPTPFYIEDILGR), different from the related mouse sequence by one amino acid..
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-Hex/HHEX Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-Hex/HHEX Antibody (PA1326).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/pa1326 1 WB anti hex antibody.jpg

Anti-Hex antibody, PA1326, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: Rat Pancreas Tissue Lysate
Lane 5: HT1080 Cell Lysate
Lane 6: MM453 Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: 6T-CEM Cell Lysate
Lane 9: COLO320 Cell Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q03014
Gene Name HHEX
Protein Name Hematopoietically-expressed homeobox protein HHEX
Tissue Specificity Liver and promyelocytic leukemia cell line HL- 60. .
Alternative Names
  • Hematopoietically expressed homeobox antibody
  • Hematopoietically-expressed homeobox protein HHEX antibody
  • HEX antibody
  • HHEX antibody
  • HHEX antibody
  • HMPH antibody
  • Homeobox hematopoietically expressed antibody
  • Homeobox protein HEX antibody
  • Homeobox protein PRH antibody
  • HOX11L PEN antibody
  • PRH antibody
  • PRHX antibody
  • Proline rich homeodomain containing transcription factor antibody
Show more
Subcellular Localization Nucleus .
Molecular Weight 30022 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (By similarity). .
Research Areas Developmental Biology, Developmental Families, Domain Families, Embryogenesis, Epigenetics And Nuclear Signaling, Lineage Markers, Lineage Specification, Stem Cells, Transcription

*You can search these to find other products in these research areas.
Background Hematopoietically-expressed homeobox protein HHEX is a protein that in humans is encoded by the HHEX gene. Homeobox genes are members of a family of transcription factors that regulate tissue development in many different organisms. Hromas et al.(1993) set out to identify homeobox genes that might play a role in hematopoiesis. And using somatic cell hybrid analysis, they mapped the HHEX gene to chromosome 10, where the HOX11 gene is located. Homeobox genes are involved in neoplastic transformation of both epithelial and hemopoietic tissues. The divergent homeobox gene HEX is expressed in the anterior visceral endoderm during early mouse development and in some adult tissues of endodermal origin, including liver and thyroid. D'Elia et al.'s findings suggested that regulation of HEX entry in the nucleus of thyrocytes may represent a critical step during human thyroid tumorigenesis.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-Hex/HHEX Antibody (PA1326)? Please let us know so we can cite the reference in this product datasheet. Email us at support@bosterbio.com.

Order Product (PA1326)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1054 $240
100ug+Free Biotin Secondary BA1003 $240

USD $240

Ships next business day.

Troubleshooting

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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.