Anti-HLA-C Antibody Picoband™

HLA C antibody

Boster Bio Anti-HLA-C Antibody Picoband™ catalog # PB9377. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PB9377
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-HLA-C Antibody Picoband™

View all HLA C Antibodies

SKU/Catalog Number

PB9377

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HLA-C Antibody Picoband™ catalog # PB9377. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HLA-C Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9377)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HLA-C.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9377 is reactive to HLA-C in Human

Applications

PB9377 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

41 kDa

Calculated molecular weight

40.649kDa

Background of HLA C

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunofluorescence, 2μg/ml, Human, -
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For HLA-C (Source: Uniprot.org, NCBI)

Gene Name

HLA-C

Full Name

HLA class I histocompatibility antigen, C alpha chain

Weight

40.649kDa

Alternative Names

C alpha chain; D6S204; FLJ27082; HLA class I histocompatibility antigen, Cw-1 alpha chain; HLAC; HLA-C; HLA-Cw; HLA-JY3; HLC-C; human leukocyte antigen-C alpha chain; leucocyte antigen C; leucocyte antigen Cw; leukocyte antigen-C alpha chain; major histocompatibility antigen HLA-C; major histocompatibility complex, class I, C; MHC class I antigen Cw*1; MHC class I antigen Cw*12; MHC class I antigen Cw*14; MHC class I antigen Cw*15; MHC class I antigen Cw*16; MHC class I antigen Cw*17; MHC class I antigen Cw*2; MHC class I antigen Cw*3; MHC class I antigen Cw*4; MHC class I antigen Cw*5; MHC cl HLA-C D6S204, HLA-JY3, HLAC, HLC-C, MHC, PSORS1 major histocompatibility complex, class I, C HLA class I histocompatibility antigen, C alpha chain|HLA-C antigen|MHC class I antigen heavy chain HLA-C|human leukocyte antigen-C alpha chain|major histocompatibility antigen HLA-C

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HLA-C, check out the HLA-C Infographic

HLA-C infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HLA-C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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21 Customer Q&As for Anti-HLA-C Antibody Picoband™

Question

Can you confirm that PB9377 detects the HLA-Cw7 and HLA-Cw12 subtypes?

Verified customer

Asked: 2023-02-02

Answer

We are uncertain if the Anti-HLA-C Antibody Picoband™ (PB9377) can detect the HLA-Cw7 and HLA-Cw12 subtypes. Our lab hasn't performed any relevant tests.

Boster Scientific Support

Answered: 2023-02-02

Question

Does PB9377 antibody binds to HLA-B exclusively (not HLA-A/C)? If so, does it recognize just one or multiple HLA-B subtypes? Which ones?

Verified customer

Asked: 2022-03-14

Answer

The Anti-HLA-C Antibody Picoband™ (PB9377) recognizes HLA-C. Our lab hasn't experimentally validated the cross-reactivity with HLA-B or HLA-C. Immunogen sequence for PB9377 is EYWDRETQKYKRQAQADRVNLRKLR.

Boster Scientific Support

Answered: 2022-03-15

Question

Does PB9377 antibody binds to HLA-B exclusively (not HLA-A/C)? If so, does it recognize just one or multiple HLA-B subtypes? Which ones?

Verified customer

Asked: 2022-03-14

Answer

The Anti-HLA-C Antibody Picoband™ (PB9377) recognizes HLA-C. Our lab hasn't experimentally validated the cross-reactivity with HLA-B or HLA-C. Immunogen sequence for PB9377 is EYWDRETQKYKRQAQADRVNLRKLR.

Boster Scientific Support

Answered: 2022-03-15

Question

We have observed staining in rat blood. Are there any suggestions? Is anti-HLA-C antibody supposed to stain blood positively?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

Based on literature blood does express HLA-C. Based on Uniprot.org, HLA-C is expressed in blood, liver, among other tissues. Regarding which tissues have HLA-C expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8655361, 12622774
Liver, Pubmed ID: 19159218, 24275569

Boster Scientific Support

Answered: 2019-12-17

Question

Do you have a BSA free version of anti-HLA-C antibody PB9377 available?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HLA-C antibody PB9377 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-03

Question

What are the validated species that confirms reactivity with the Anti-HLA-C Antibody Picoband?

Verified customer

Asked: 2019-11-11

Answer

The Anti-HLA-C Antibody Picoband cross reacts with Human, rat and mouse samples.

Boster Scientific Support

Answered: 2019-11-12

Question

Can you help my question with product PB9377, anti-HLA-C antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9377 anti-HLA-C antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-07

Question

I was wanting to use your anti-HLA-C antibody for IHC-P for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

Verified Customer

Verified customer

Asked: 2019-11-04

Answer

You can see on the product datasheet, PB9377 anti-HLA-C antibody has been tested for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-04

Question

See attached the WB image, lot number and protocol we used for liver using anti-HLA-C antibody PB9377. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-11

Question

Will PB9377 anti-HLA-C antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Parker

Verified customer

Asked: 2019-09-04

Answer

As indicated on the product datasheet, PB9377 anti-HLA-C antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-04

Question

We were well pleased with the WB result of your anti-HLA-C antibody. However we have been able to see positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is HLA-C supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-02-18

Answer

Based on literature, liver does express HLA-C. Generally HLA-C expresses in cell membrane. Regarding which tissues have HLA-C expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8655361, 12622774
Liver, Pubmed ID: 19159218, 24275569

Boster Scientific Support

Answered: 2019-02-18

Question

Is a blocking peptide available for product anti-HLA-C antibody (PB9377)?

Verified Customer

Verified customer

Asked: 2018-12-07

Answer

We do provide the blocking peptide for product anti-HLA-C antibody (PB9377). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-12-07

Question

We are currently using anti-HLA-C antibody PB9377 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-09-25

Answer

The anti-HLA-C antibody (PB9377) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-09-25

Question

I see that the anti-HLA-C antibody PB9377 works with IHC-P, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-09-18

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-09-18

Question

I would like to test anti-HLA-C antibody PB9377 on human liver for research purposes, then I may be interested in using anti-HLA-C antibody PB9377 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-08-30

Answer

The products we sell, including anti-HLA-C antibody PB9377, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-08-30

Question

Does anti-HLA-C antibody PB9377 work for IHC-P with liver?

D. Carter

Verified customer

Asked: 2017-12-22

Answer

According to the expression profile of liver, HLA-C is highly expressed in liver. So, it is likely that anti-HLA-C antibody PB9377 will work for IHC-P with liver.

Boster Scientific Support

Answered: 2017-12-22

Question

Is this PB9377 anti-HLA-C antibody reactive to the isotypes of HLA-C?

G. Johnson

Verified customer

Asked: 2017-12-01

Answer

The immunogen of PB9377 anti-HLA-C antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human HLA-C(38-62aa EYWDRETQKYKRQAQADRVNLRKLR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-12-01

Question

Does PB9377 cross-react with other HLA Classes?

Verified Customer

Verified customer

Asked: 2017-06-22

Answer

PB9377 is not specific to HLA-C only. It cross reacted with other HLA classes, such as HLA-B.

Boster Scientific Support

Answered: 2017-06-22

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-HLA-C antibody PB9377. Let me know if you need anything else.

A. Wu

Verified customer

Asked: 2016-08-03

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-08-03

Question

We have tried in the past anti-HLA-C antibody for Flow Cytometry on blood in the past. I am using rat, and We intend to use the antibody for IHC-P next. We need examining blood as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

E. Edwards

Verified customer

Asked: 2014-09-25

Answer

I have checked the website and datasheets of our anti-HLA-C antibody and I see that PB9377 has been validated on rat in both Flow Cytometry and IHC-P. Thus PB9377 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-09-25

Question

Will this antibody react with C* 05:01?

Verified Customer

Verified customer

Asked: 2014-03-25

Answer

According to the following sequence of Cw*05:01, the immunogen sequence of PB9377 shares 96% sequence homolog with Cw*05:01. https://www.uniprot.org/uniprot/Q9TNN7#sequences. We are unable to experimentally verify if PB9377 reacts with C* 05:01.

Boster Scientific Support

Answered: 2014-03-25

Order DetailsPrice
PB9377

100μg

$370
PB9377-10ug

10μg sample (liquid)

$99
PB9377-Biotin

100 μg Biotin conjugated

$570
PB9377-Cy3

100 μg Cy3 conjugated

$570
PB9377-Dylight488

100 μg Dylight488 conjugated

$570
PB9377-Dylight550

100 μg Dylight550 conjugated

$570
PB9377-Dylight594

100 μg Dylight594 conjugated

$570
PB9377-FITC

100 μg FITC conjugated

$570
PB9377-HRP

100 μg HRP conjugated

$570
PB9377-APC

100 μg APC conjugated

$670
PB9377-PE

100 μg PE conjugated

$670
PB9377-carrier-free

Carrier Free

$370

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