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HMGB1 antibody (A00066-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 25 Publications

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a00066 1 hmgb1 primary antibodies fcm testing 11

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Flow Cytometry analysis of THP-1 cells using anti-HMGB1 antibody (A00066-1).
Overlay histogram showing THP-1 cells stained with A00066-1 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HMGB1 Antibody (A00066-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Designing a Western blot for HMGB1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the HMGB1 Western Blot Guide

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Anti-HMGB1 Antibody Picoband®

Name: Anti-HMGB1 Antibody Picoband®
Brand: Boster Bio
SKU: A00066-1
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

HMGB1 antibody

25 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

HMGB1 antibody was positively detected by WB in:

human Hela whole cell, human 293T whole cell, human K562 whole cell, human Jurkat whole cell, rat brain tissue, mouse brain tissue, mouse RAW264.7- WT whole cell, mouse RAW264.7-Hmgb1 KO whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

HMGB1 antibody was positively detected by IHC in:

mouse intestine tissue, mouse liver tissue, rat intestine tissue, rat liver tissue, human mammary cancer tissue, human placenta tissue,

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

HMGB1 antibody was positively detected by ICC/IF in:

U20S cell, A431 cell

HMGB1 antibody was positively detected by FC in:

THP-1 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-HMGB1 Antibody Picoband®
View all HMGB1 Antibodies

Form

Lyophilized

Uniprot

P09429

Gene ID

3146

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HMGB1, identical to the related mouse and rat sequences.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

25 kDa

Calculated Molecular Weight

24.9 kDa

Background of HMGB1

High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that in humans is encoded by the HMGB1 gene. This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Cite This Product

Anti-HMGB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00066-1)

Protocols

Western Blot protocol for HMGB1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for HMGB1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HMGB1 antibody

Review Protocol

Flow Cytometry (FC) protocol for HMGB1 antibody

Review Protocol

Publications

Enzyme/Reactive Oxygen Species-Dually Activated Hyaluronic Acid Nanocarriers Enable Celastrol Delivery for Site-Specific Therapy of Inflammatory Bowel Diseases and Colorectal Cancer

Published: 2026-08-10

Journal: International Journal of Nanomedicine

Application: IF

Impact Factor: 8.70

Species: Mouse

PMID: 42602668

SKU	A00066-1
Application	Immunofluorenscence
Sample	Mouse 4T1 cell climbing slides
Sample Processing Description	Mouse 4T1 cells fixed with 4% paraformaldehyde for 15 minutes
Primary Antibody	Anti-HMGB1 Antibody Picoband®
Primary Incubation	1:500, overnight at 4 ℃
Blocking Agent	Goat serum
Secondary Antibody	DyLight 550-conjugated goat anti-rabbit antibody.
Secondary Incubation	Incubate at room temperature for 1 hour
Detection	Laser confocal microscopy
Results Summary	The ordering and delivery cycle of the antibodies is very short — I was able to receive the products within a week, which saved a lot of time. The pre-sales and after-sales services are convenient and efficient. Since my background is in chemistry, I consulted Boster’s technical team about many details of biological experiments. The technical specialists were patient and thorough in their analysis and explanations, which helped me avoid many detours during the actual experimental procedures. Most importantly, these antibodies offer a high cost-performance ratio, with good binding performance, excellent imaging quality, and a high experimental success rate, providing a solid foundation for conducting related experiments.

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Anti-HMGB1 Antibody Picoband®

HMGB1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Enzyme/Reactive Oxygen Species-Dually Activated Hyaluronic Acid Nanocarriers Enable Celastrol Delivery for Site-Specific Therapy of Inflammatory Bowel Diseases and Colorectal Cancer

Inflachromene attenuates monocrotaline-induced pulmonary arterial hypertension by suppressing the HMGB1–TLR4/RAGE–NF-κB signaling pathway

A self-amplifying cuproptosis nanomedicine to overcome immunosuppression by blocking tumor-derived exosomes for enhancing lung cancer immunotherapy

Well-defined electronic configuration cyano-bridged bimetallic nanozyme for cancer catalytic-immunotherapy

Biomimetic-Nanoparticle-Enhanced Photothermal Immunotherapy: Targeted Delivery of Near-Infrared Region II Agents and Immunoadjuvants for Tumor Immunogenicity

HMGB1/TREM1 crosstalk between heat-injured hepatocytes and macrophages promotes HCC progression after RFA

SHARPIN contributes to sevoflurane-induced neonatal neurotoxicity through up-regulating HMGB1 to repress M2 like-macrophage polarization

Mn2+/CpG Oligodeoxynucleotides Codecorated Black Phosphorus Nanosheet Platform for Enhanced Antitumor Potency in Multimodal Therapy

n-3 polyunsaturated fatty acids alleviate the progression of obesity-related osteoarthritis and protect cartilage through inhibiting the HMGB1-RAGE/TLR4 signaling pathway

Time-programmed activation of CD47 disruption and immunogenic cell death with Cas9 ribonucleoprotein nanocapsule for improved cancer immunotherapy

Voluntarily wheel running inhibits the growth of CRPC xenograft by inhibiting HMGB1 in mice

HMGB1 promotes the development of castration‑resistant prostate cancer by regulating androgen receptor activation

HMGB1 in the mPFC governs comorbid anxiety in neuropathic pain

Short-term pretreatment of naringin isolated from Citrus wilsonii Tanaka attenuates rat myocardial ischemia/reperfusion injury

Urolithin A protects against acetaminophen-induced liver injury in mice via sustained activation of Nrf2

Citrus fruits are rich in flavonoids for immunoregulation and potential targeting ACE2

Original research: Extracellular HMGB1 blockade inhibits tumor growth through profoundly remodeling immune microenvironment and enhances checkpoint inhibitor-based immunotherapy

HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway

Bioinformatic and biochemical findings disclosed anti-hepatic steatosis mechanism of calycosin

Protective effects of mild hypothermia against hepatic injury in rats with acute liver failure

Galantamine protects against hydrochloric acid aspirationinduced acute respiratory distress syndrome in rabbits

Celastrol pretreatment attenuates rat myocardial ischemia/ reperfusion injury by inhibiting high mobility group box 1 protein expression via the PI3K/Akt pathway

Short-Term Hesperidin Pretreatment Attenuates Rat Myocardial Ischemia/Reperfusion Injury by Inhibiting High Mobility Group Box 1 Protein Expression via the PI3K/Akt Pathway

Effects of Ethyl Pyruvate in Preventing the Development of Diet-induced Atherosclerosis by Blocking the HMGB1 Expression in ApoE-Deficient Mice

Association of Upregulated HMGB1 and c-IAP2 Proteins With Hepatocellular Carcinoma Development and Progression

Review

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1 Review For Anti-HMGB1 Antibody Picoband®

Accurate localization and good imaging quality

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