Anti-TNF alpha Antibody

Boster Bio Anti-TNF alpha Antibody catalog # RP1000. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. Cited in 63 publication(s).

Product Info Summary

SKU: RP1000
Size: 100μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-TNF alpha Antibody

See all TNF-alpha products

SKU/Catalog Number

RP1000

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TNF alpha Antibody catalog # RP1000. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TNF alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1000)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse TNF alpha recombinant protein (Position: L80-L235).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1000 is reactive to Tnf in Mouse

Applications

RP1000 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Tnf (Source: Uniprot.Org, NCBI)

Uniprot ID

P06804

Gene ID

21926

Gene Name

Tnf

Full Name

Tumor necrosis factor

Weight

25.896kDa

Superfamily

tumor necrosis factor family

Alternative Names

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha Tnf|DI, DIF, TNF-, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tna, Tnfs, Tnfsf1a, Tnlg1f, Tnf|tumor necrosis factor|tumor necrosis factor|cachectin|tumor necrosis factor ligand 1f|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnf, check out the Tnf Infographic

Tnf infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1000 has been cited in 63 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lan Q,Lu R,Chen H,Pang Y, Xiong F,Shen C,Qin Z,Zheng L,Xu G,Zhao J.MMP-13 enzyme and pH responsive theranostic nanoplatform for osteoarthritis.J Nanobiotechnology.2020 Aug 27;18(1):117.doi:10.1186/s12951-020-00666-7. PMID:32854712;PMCID:PMC7450974.
Species: Mouse
RP1000 usage in article: APP:IF, SAMPLE:CHONDROCYTES, DILUTION:1:100

Resveratrol attenuates hyperoxia%u2010induced oxidative stress, inflammation and fibrosis and suppresses Wnt/%u03B2%u2010catenin signalling in lungs of neonatal rats

Efficacy of acetylshikonin in preventing obesity and hepatic steatosis in db/db mice

Protective effects of baicalin on carbon tetrachloride induced liver injury by activating PPAR%u03B3 and inhibiting TGF%u03B21

Immunomodulatory properties of quercetin-3-O-%u03B1-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus

Neuroprotective effects of %u03B1-lipoic acid on long-term experimental autoimmune encephalomyelitis.

Polymeric micelles for potentiated antiulcer and anticancer activities of naringin

Rhein inhibits lipopolysaccharide-induced intestinal injury during sepsis by blocking the toll-like receptor 4 nuclear factor-%u03BAB pathway

Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated %u2026

Characterization of acetaminophen-induced cytotoxicity in target tissues

Carbon monoxide-releasing molecule-3 protects against ischemic stroke by suppressing neuroinflammation and alleviating blood-brain barrier disruption

BML-111 reduces neuroinflammation and cognitive impairment in mice with sepsis via the SIRT1/NF-%u03BAB signaling pathway.

Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response

Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model

Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats

CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment

Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication

Effect of Puerarin on Expression of ICAM-1 and TNF-? in Kidneys of Diabetic Rats

Hydrogen sulfide accelerates wound healing in diabetic rats

Molecular responses of radiation-induced liver damage in rats

Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye

Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway

Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation

Comparison of Serum Levels of Vitamin D and Inflammatory Markers Between Women With Gestational Diabetes Mellitus and Healthy Pregnant Control

Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A

Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury

Neuroprotective effect of pretreatment with ganoderma lucidum in cerebral ischemia/reperfusion injury in rat hippocampus

Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke

Gu R, Ding X, Tang W, Lei B, Jiang C, Xu G. Front Pharmacol. 2018 Apr 6;9:331. doi: 10.3389/fphar.2018.00331. eCollection 2018. A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal M

Liu Hp, Gao Zh, Cui Sx, Sun Df, Wang Y, Zhao Cr, Lou Hx, Qu Xj. Plos One. 2012;7(3):E33243. Doi: 10.1371/Journal.Pone.0033243. Epub 2012 Mar 14. Inhibition Of Intestinal Adenoma Formation In Apc(Min/+) Mice By Riccardin D, A Natural Product Derive...

Lu Nn, Liu Q, Gu Lg, Ge Sj, Wu J, Ze-Ji Q, Qiu Zj, Zhang Hc, Chao Ex, Yu Zn. Evid Based Complement Alternat Med. 2014;2014:976364. Doi: 10.1155/2014/976364. Epub 2014 Jan 8. Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Ac...

Wu W, Su M, Li T, Wu K, Wu X, Tang Z. Int Immunopharmacol. 2015 Sep;28(1):182-7. Doi: 10.1016/J.Intimp.2015.06.003. Epub 2015 Jun 10. Cantharidin-Induced Liver Injuries In Mice And The Protective Effect Of Vitamin C Supplementation.

Qiang G, Zhang L, Yang X, Xuan Q, Shi L, Zhang H, Chen B, Li X, Zu M, Zhou D, Guo J, Yang H, Du G. Eur J Pharmacol. 2012 Jun 15;685(1-3):156-64. Doi: 10.1016/J.Ejphar.2012.04.028. Epub 2012 Apr 21. Effect Of Valsartan On The Pathological Progressi...

Sun J, Chen Lj, Zhang Gb, Jiang Jt, Zhu M, Tan Y, Wang Ht, Lu Bf, Zhang Xg. Cancer Immunol Immunother. 2010 Aug;59(8):1163-71. Doi: 10.1007/S00262-010-0841-1. Epub 2010 Mar 24. Clinical Significance And Regulation Of The Costimulatory Molecule B7-...

Xie Jb, Zhang X, Li Qh, Xu Zj. Neural Regen Res. 2015 Feb;10(2):219-24. Doi: 10.4103/1673-5374.152374. Inhibition Of Inflammatory Cytokines After Early Decompression May Mediate Recovery Of Neurological Function In Rats With Spinal Cord Injury.

Xia W, Li Dw, Xiang L, Chang Jj, Xia Zl, Han Ej. Chin J Physiol. 2015 Apr 30;58(2):104-13. Doi: 10.4077/Cjp.2015.Bad273. Neuroprotective Effects Of An Aqueous Extract Of Futokadsura Stem In An A??-Induced Alzheimer'S Disease-Like Rat Model.

Li H, Qiu P, Wang J, Niu C, Pan S. Food Funct. 2015 Feb;6(2):470-8. Doi: 10.1039/C4Fo00739E. Effects Of Compound Ginkgo Biloba On Intestinal Permeability In Rats With Alcohol-Induced Liver Injury.

Li C, Chen X, Zhang N, Song Y, Mu Y. Neural Regen Res. 2012 Feb 15;7(5):325-31. Doi: 10.3969/J.Issn.1673-5374.2012.05.001. Gastrodin Inhibits Neuroinflammation In Rotenone-Induced Parkinson'S Disease Model Rats.

Jiang Gt, Chen X, Li D, An Hx, Jiao Jd. Mol Med Rep. 2014 Sep;10(3):1501-8. Doi: 10.3892/Mmr.2014.2323. Epub 2014 Jun 13. Ulinastatin Attenuates Renal Interstitial Inflammation And Inhibits Fibrosis Progression In Rats Under Unilateral Ureteral Ob...

Li G, Ren J, Wang G, Gu G, Hu D, Ren H, Hong Z, Wu X, Liu S, Li J. Int Immunopharmacol. 2014 Feb;18(2):244-8. Doi: 10.1016/J.Intimp.2013.12.014. Epub 2013 Dec 22. T2 Enhances In Situ Level Of Foxp3+ Regulatory Cells And Modulates Inflammatory Cyto...

Jin Hb, Yang Yb, Song Yl, Zhang Yc, Li Yr. Mol Biol Rep. 2012 Dec;39(12):11005-9. Doi: 10.1007/S11033-012-2002-4. Epub 2012 Oct 8. Protective Roles Of Quercetin In Acute Myocardial Ischemia And Reperfusion Injury In Rats.

Ye Q, Zheng Y, Fan S, Qin Z, Li N, Tang A, Ai F, Zhang X, Bian Y, Dang W, Huang J, Zhou M, Zhou Y, Xiong W, Yan Q, Ma J, Li G. Plos One. 2014 Jul 24;9(7):E103298. Doi: 10.1371/Journal.Pone.0103298. Ecollection 2014. Lactoferrin Deficiency Promotes...

Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.

Huang Q, Du J, Fan J, Lv Z, Qian X, Zhang X, Han J, Chen C, Wu F, Jin Y. Med Oncol. 2014 Sep;31(9):144. Doi: 10.1007/S12032-014-0144-Z. Epub 2014 Aug 12. The Effect Of Proinflammatory Cytokines On Il-17Ra Expression In Nsclc.

Guo J, Li F, Wu Q, Gong Q, Lu Y, Shi J. Phytomedicine. 2010 Oct;17(12):950-5. Doi: 10.1016/J.Phymed.2010.03.007. Epub 2010 Apr 9. Protective Effects Of Icariin On Brain Dysfunction Induced By Lipopolysaccharide In Rats.

Cen J, Liu L, Li Ms, He L, Wang Lj, Liu Yq, Liu M, Ji Bs. J Pharm Pharmacol. 2013 May;65(5):665-72. Doi: 10.1111/Jphp.12033. Epub 2013 Feb 26. Alteration In P-Glycoprotein At The Blood-Brain Barrier In The Early Period Of Mcao In Rats.

Cui Wy, Tian Ay, Bai T. Clin Exp Pharmacol Physiol. 2011 Nov;38(11):747-54. Doi: 10.1111/J.1440-1681.2011.05584.X. Protective Effects Of Propofol On Endotoxemia-Induced Acute Kidney Injury In Rats.

Ran J, Ma J, Liu Y, Tan R, Liu H, Lao G. J Diabetes Res. 2014;2014:287536. Doi: 10.1155/2014/287536. Epub 2014 Mar 13. Low Protein Diet Inhibits Uric Acid Synthesis And Attenuates Renal Damage In Streptozotocin-Induced Diabetic Rats.

Li Q, Xia Yy, Tang Jc, Wang Ry, Bei Cy, Zeng Y. Artif Cells Blood Substit Immobil Biotechnol. 2011 Jun;39(3):137-42. Doi: 10.3109/10731199.2010.502880. Epub 2010 Jul 23. In Vitro And In Vivo Biocompatibility Investigation Of Diamond-Like Carbon Co...

Jiang Zy, Guo Yy, Ren Hb, Zou Yf, Fan Ms, Lv Y, Han P, De W, Sun Lz. Placenta. 2012 Jan;33(1):1-7. Doi: 10.1016/J.Placenta.2011.09.004. Epub 2011 Nov 1. Tumor Necrosis Factor (Tnf)-?? Upregulates Progesterone Receptor-A By Activating The Nf-??b Si...

Zhang Y, Zhang Y, Xie Y, Gao Y, Ma J, Yuan J, Li J, Wang J, Li L, Zhang J, Chu L. J Ethnopharmacol. 2013 Jul 9;148(2):671-81. Doi: 10.1016/J.Jep.2013.05.028. Epub 2013 May 22. Multitargeted Inhibition Of Hepatic Fibrosis In Chronic Iron-Overloaded...

Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.

Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Int J Mol Sci. 2012;13(7):8379-87. Doi: 10.3390/Ijms13078379. Epub 2012 Jul 5. Astragalus Membranaceus Inhibits Inflammation Via Phospho-P38 Mitogen-Activated Protein Kinase (Mapk) And Nuclear Factor (Nf)-...

Zhang S, Liu X, Goldstein S, Li Y, Ge J, He B, Fei X, Wang Z, Ruiz G. Mol Med Rep. 2013 Jan;7(1):93-8. Doi: 10.3892/Mmr.2012.1159. Epub 2012 Oct 29. Role Of The Jak/Stat Signaling Pathway In The Pathogenesis Of Acute Myocardial Infarction In Rats ...

Jiang Z, Han B, Li H, Yang Y, Liu W. Carbohydr Polym. 2015 Sep 20;129:1-8. Doi: 10.1016/J.Carbpol.2015.04.040. Epub 2015 Apr 27. Carboxymethyl Chitosan Represses Tumor Angiogenesis In Vitro And In Vivo.

Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.

Zhou Ch, Wan Yy, Chu Xh, Song Z, Xing Sh, Wu Yq, Yin Xx. Oncol Lett. 2012 Dec;4(6):1259-1263. Epub 2012 Sep 21. Urotensin Ii Contributes To The Formation Of Lung Adenocarcinoma Inflammatory Microenvironment Through The Nf-??b Pathway In Tumor-Bear...

Chen Yf, Zhao Zq, Wu Zm, Zou Zy, Luo Xj, Li J, Xie C, Liang Y. Int J Clin Exp Pathol. 2014 Dec 1;7(12):8411-20. Ecollection 2014. The Role Of Rip1 And Rip3 In The Development Of Aplastic Anemia Induced By Cyclophosphamide And Busulphan In Mice.

Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients.

Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...

Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...

Wu Yh, Hu Sq, Liu J, Cao Hc, Xu W, Li Yj, Li Lj. Int J Mol Med. 2014 Jun;33(6):1498-506. Doi: 10.3892/Ijmm.2014.1730. Epub 2014 Apr 7. Nature And Mechanisms Of Hepatocyte Apoptosis Induced By D-Galactosamine/Lipopolysaccharide Challenge In Mice.

Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

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21 Customer Q&As for Anti-TNF alpha Antibody

Question

I have attached the WB image, lot number and protocol we used for prostatic carcinoma using anti-TNF alpha antibody RP1000. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-10

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostatic carcinoma using anti-TNF alpha antibody RP1000. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-24

Question

My question regarding product RP1000, anti-TNF alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Jackson

Verified customer

Asked: 2020-02-14

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1000 anti-TNF alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-14

Question

Do you have a BSA free version of anti-TNF alpha antibody RP1000 available?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TNF alpha antibody RP1000 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-30

Question

We have seen staining in mouse prostatic carcinoma. Any tips? Is anti-TNF alpha antibody supposed to stain prostatic carcinoma positively?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

From literature prostatic carcinoma does express TNF. From Uniprot.org, TNF is expressed in leukocyte, blood, prostatic carcinoma, among other tissues. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166

Boster Scientific Support

Answered: 2020-01-29

Question

you antibody to test anti-TNF alpha antibody RP1000 on mouse prostatic carcinoma for research purposes, then I may be interested in using anti-TNF alpha antibody RP1000 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

The products we sell, including anti-TNF alpha antibody RP1000, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-01

Question

Our lab were well pleased with the WB result of your anti-TNF alpha antibody. However we have been able to see positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is TNF supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

Based on literature, leukocyte does express TNF. Generally TNF expresses in cell membrane. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166

Boster Scientific Support

Answered: 2019-11-18

Question

Is a blocking peptide available for product anti-TNF alpha antibody (RP1000)?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

We do provide the blocking peptide for product anti-TNF alpha antibody (RP1000). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-08

Question

Is this RP1000 anti-TNF alpha antibody reactive to the isotypes of TNF?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

The immunogen of RP1000 anti-TNF alpha antibody is E. coli-derived mouse TNF alpha recombinant protein(Position: L80-L235). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-27

Question

We had a look in your products and it seems that RP1000 Anti-TNF alpha Antibody is the most suitable for our research. Would you be able to please give us the epitopes of this antibody?

Verified Customer

Verified customer

Asked: 2019-05-09

Answer

I am sorry to tell you that there is no available information about the epitope recognized by RP1000 Anti-TNF alpha Antibody, as it has not been mapped yet.

Boster Scientific Support

Answered: 2019-05-09

Question

I would be interested in your anti TNF antibody (RP1000). Does it recognize both the cleaved and full length forms of TNF or only the cleaved one? Does it cross-react with human? I need an antibody that does NOT recognize the human TNF.

Verified Customer

Verified customer

Asked: 2019-04-17

Answer

We didn't obtain the target band by working on tissue or cell samples. We tested the recombinant protein and didn't know if RP1000 recognized the cleaved one or full length forms of TNF. We have another anti-mouse TNF alpha antibody A00002-4 which recognize full length forms of TNF. https://www.bosterbio.com/anti-tnf-alpha-picoband-trade-antibody-a00002-4-boster.html?options=cart A00002-4 doesn't cross react with human.

Boster Scientific Support

Answered: 2019-04-17

Question

Will anti-TNF alpha antibody RP1000 work for WB with prostatic carcinoma?

Verified Customer

Verified customer

Asked: 2019-03-11

Answer

According to the expression profile of prostatic carcinoma, TNF is highly expressed in prostatic carcinoma. So, it is likely that anti-TNF alpha antibody RP1000 will work for WB with prostatic carcinoma.

Boster Scientific Support

Answered: 2019-03-11

Question

Would anti-TNF alpha antibody RP1000 work on dog ELISA with blood?

Verified Customer

Verified customer

Asked: 2019-01-04

Answer

Our lab technicians have not validated anti-TNF alpha antibody RP1000 on dog. You can run a BLAST between dog and the immunogen sequence of anti-TNF alpha antibody RP1000 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog blood in ELISA, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-01-04

Question

To Boster Technical Support; I am looking for antibodies against neutrophils and TNF-alpha for Immunohistochemistry. I came across in your product RP1000 Anti-TNF alpha Antibody that can be used for staining. But, I have few questions. Do these antibodies work on tissue sections of knee joint? Knee joints are 4% formaldehyde fixed and paraffin embedded sections. Before embedding, knee joints are decalcified with 20% formic acid + Sodium citrate for 3 days. Do these antibodies work on this particular treated sections?

Verified Customer

Verified customer

Asked: 2018-04-27

Answer

Thank you for your inquiry, unfortunately, we were not able to test every organ or tissue when we establish the product. However, if the knee joints were properly decalcified and the species is tested, we don't see any problems why these antibodies should not work in these sections.

Boster Scientific Support

Answered: 2018-04-27

Question

We are currently using anti-TNF alpha antibody RP1000 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-26

Answer

The anti-TNF alpha antibody (RP1000) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-26

Question

I need help, I already received the products RP1000 Anti-TNF alpha Antibody this week and I run to detect the TNF-alpha that is supposed to be overexpressed in some transfected cells, but the results in WB I got strange band around 40 KD and no bands at the position of 17 KD.

Verified Customer

Verified customer

Asked: 2017-08-28

Answer

The TNF-alpha is known to form a homotrimer, the expected molecular weight for that band would be around 58 kDa. In order for us to help you more about the nature of the band you are observing, it would be helpful if you could provide us some additional details about your protocol. Including; what type of cells were you staining? What species was the transfected TNF alpha? Was the transfected protein tagged? How were the cells lysed? How much protein was loaded per lane, and were the samples reduced and denatured? What blocking agent you used? What was the primary antibody dilution and incubation time? What secondary antibody was used? What was the dilution and incubation time, and has this secondary antibody been working well with other primary antibodies?

Boster Scientific Support

Answered: 2017-08-28

Question

Does RP1000 anti-TNF alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-05-17

Answer

It shows on the product datasheet, RP1000 anti-TNF alpha antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-05-17

Question

I see that the anti-TNF alpha antibody RP1000 works with WB, what is the protocol used to produce the result images on the product page?

H. Johnson

Verified customer

Asked: 2017-01-31

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-01-31

Question

Our lab used your anti-TNF alpha antibody for ELISA on blood a few months ago. I am using mouse, and I plan to use the antibody for WB next. We are interested in examining blood as well as prostatic carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

D. Jones

Verified customer

Asked: 2016-10-04

Answer

I looked at the website and datasheets of our anti-TNF alpha antibody and I see that RP1000 has been tested on mouse in both ELISA and WB. Thus RP1000 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2016-10-04

Question

I was wanting to use your anti-TNF alpha antibody for WB for mouse prostatic carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse prostatic carcinoma identification?

D. Parker

Verified customer

Asked: 2015-10-22

Answer

You can see on the product datasheet, RP1000 anti-TNF alpha antibody has been tested for ELISA, IHC, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse prostatic carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-10-22

Question

I would like to investigate TNF-alpha expression rat perivascular adipose tissue using immunohistochemistry (paraffin sections) and western blotting. Do you know if your product RP1000 Anti-TNF alpha Antibody would be suitable to determine expression in rat PVAT or any other white adipose tissue?

Verified Customer

Verified customer

Asked: 2013-06-03

Answer

Yes, I am glad to say that our products RP1000 Anti-TNF alpha Antibody is tested and guaranteed for rat in WB and IHC-P. However, in regards to the perivascular adipose tissues, we do not have any records about it yet, so we suggest running a positive control with a tissue that is known for its expression of TNF alpha

Boster Scientific Support

Answered: 2013-06-03

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